Microscopy Core

项目总结/摘要 第三阶段显微镜核心的目标是为COBRE研究人员提供成像工具和培训， 使他们的心血管研究成功和可持续。核心将协助调查人员收集- 使用显微图像，这将有助于他们的研究出版，获得进一步的资金，并 阐述新的见解，推动他们的研究在新的方向。核心将提供最先进的 设备和专业知识，在各种技术，包括组织学和组织化学分析，免疫， 组织化学和免疫细胞化学，原位杂交，显微镜，计算机辅助图像 分析和形态测定，活细胞成像，活体转盘共聚焦和多光子显微镜， 透射电子显微镜，免疫金标记，和先进的冷冻技术，如冷冻骨折， 真实和冷冻蚀刻。显微镜核心设备和工作人员位于OMRF Cardio中心， 血管生物学研究计划，靠近COBRE实验室和心血管表型- ing核心。这个中心位置促进了核心用户之间的高水平互动。核心将是 由两位影像学专家Florea Lupu博士和Courtney Griffin博士在三位经验丰富的 工作人员。在第一阶段和第二阶段成功的基础上，显微镜核心将扩大和加速 研究效率和COBRE研究人员之间的合作。

PROJECT SUMMARY/ABSTRACT The goal of the Phase III Microscopy Core is to provide COBRE investigators with imaging tools and training to make their cardiovascular research successful and sustainable. The Core will assist investigators with collect- ing microscopic images that will facilitate publication of their research, acquisition of further funding, and dis- covery of novel insights that push their research in new directions. The Core will provide state-of-the-art equipment and expertise in a variety of techniques, including histological and histochemical analyses, immu- nohistochemistry and immunocytochemistry, in-situ hybridization, photomicroscopy, computer-assisted image analysis and morphometry, live-cell imaging, intravital spinning-disk confocal and multiphoton microscopy, transmission electron microscopy, immunogold labeling, and advanced cryotechnologies such as freeze frac- ture and freeze etching. The Microscopy Core equipment and staff are centrally located in the OMRF Cardio- vascular Biology Research Program, in close proximity to the COBRE labs and the Cardiovascular Phenotyp- ing Core. This central location promotes a high level of interaction among Core users. The Core will be di- rected by two imaging experts, Drs. Florea Lupu and Courtney Griffin, with assistance from three experienced staff members. Building on its success during Phases I and II, the Microscopy Core will expand and accelerate research productivity and collaboration among COBRE investigators.