Structural and Functional Mechanisms of PTH-Receptor Signaling

PTH 受体信号传导的结构和功能机制

• 批准号: 9282434
• 负责人: Guillermo Alberto Calero
• 金额: $ 48.5万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9282434
• 关键词: Affinity; Animals; Arrestins; Behavior; Binding; Calcium; Cause of Death; Cell physiology; Cell surface; Cells; Complex; Crystallization; Crystallography; Cyclic AMP; Data; Development; Diagnostic radiologic examination; Disease; Drug Targeting; Early Endosome; Endosomes; G-Protein-Coupled Receptors; G-substrate; GTP-Binding Protein alpha Subunits, Gs; GTP-Binding Proteins; Goals; Homeostasis; Hypocalcemia result; Hypoparathyroidism; Knowledge; Length; Ligands; Location; Measurement; Mediating; Medical; Minerals; Molecular; Molecular Conformation; Mus; Osteoporosis; PTH gene; Parathyroid Hormone Receptor; Process; Production; Property; Proteins; Receptor Signaling; Regulation; Research; Resolution; Role; Side; Signal Transduction; Structure; Testing; Treatment Efficacy; United States; United States National Institutes of Health; analog; base; bone; bone cell; bone turnover; clinically relevant; disability; experimental study; hormone analog; insight; kidney cell; nanocrystal; novel therapeutic intervention; parathyroid hormone (1-34); preclinical development; programs; public health relevance; receptor; receptor binding; receptor internalization; response; therapeutic target; therapy development; x-ray free-electron laser

DESCRIPTION (provided by applicant): The goal of this proposed research is to determine the structural and cellular basis underlying the mechanism of parathyroid hormone (PTH) receptor (PTHR) signaling. The PTHR is a major G protein-coupled receptor (GPCR) that regulates Ca2+ homeostasis and bone turnover and is the most effective therapeutic target for osteoporosis. The recently recognized capacity of PTH and certain PTH analogs to prolong G-protein activity and cAMP production after PTHR internalization into early endosomes has drastically changed how we think about cellular signaling of the PTHR, and how we study drugs that target this receptor. This new and unexpected behavior of a GPCR is of particular relevance for understanding how the recently developed long-acting PTH analogs, including LA-PTH that is in preclinical development for the treatment of hypoparathyroidism, induce remarkable prolonged signaling (cAMP and calcium) responses in cells and in mice. The structural determinants of the PTHR and cellular mechanisms responsible for these actions are not known and this is an obstacle to further progress for identifying clinically relevant analogs. We therefore propose a research program to overcome this obstacle. Two specific aims are proposed: Aim 1 determines the structural determinants of PTHR action through crystallography of full-length PTHR, and PTHR bound to PTH, or to LA-PTH. We will focus initially on the structural basis for the different functional properties of PTH ligands and on ionic interactions likely to be sensitive to pH changes encountered in endosomes because our preliminary findings support a critical role of endosomal pH on sustained PTHR signaling. This structural and molecular information will be further applied to Aim 2, which determines the cellular mechanism regulating endosomal PTHR signaling, focusing on the role of low pH conditions found in the endosome in determining the stability of PTH-PTHR-G protein complexes. Knowledge of structural details and differences of how PTH and LA-PTH bind to the PTHR and trigger signaling in specific subcellular locations (endosomes) provides insights into molecular and cellular processes, which can provide new opportunities for therapy. Selective targeting of PTHR-mediated endosomal Gs signaling might offer more effective and selective treatments than global targeting of cell-surface signaling.

描述（由申请人提供）：这项拟议研究的目标是确定甲状旁腺激素（PTH）受体（PTHR）信号传导机制的结构和细胞基础。PTHR是一种主要的G蛋白偶联受体（GPCR），调节Ca 2+稳态和骨转换，是骨质疏松症最有效的治疗靶点。最近认识到PTH和某些PTH类似物在PTHR内化到早期内体后延长G蛋白活性和cAMP产生的能力，这极大地改变了我们对PTHR细胞信号传导的看法，以及我们如何研究靶向这种受体的药物。GPCR的这种新的和意想不到的行为对于理解最近开发的长效PTH类似物（包括处于临床前开发中用于治疗甲状旁腺功能减退症的LA-PTH）如何在细胞和小鼠中诱导显著延长的信号传导（cAMP和钙）应答特别相关。PTHR的结构决定因素和负责这些作用的细胞机制尚不清楚，这是鉴定临床相关类似物的进一步进展的障碍。因此，我们提出了一个研究计划，以克服这一障碍。提出了两个具体目标：目标1通过全长PTHR的晶体学和与PTH或LA-PTH结合的PTHR确定PTHR作用的结构决定因素。我们将首先关注PTH配体的不同功能特性的结构基础和离子相互作用可能对内体中遇到的pH变化敏感，因为我们的初步研究结果支持内体pH对持续PTHR信号传导的关键作用。这些结构和分子信息将进一步应用于目标2，其确定调节内体PTHR信号传导的细胞机制，重点是内体中发现的低pH条件在确定PTH-PTHR-G蛋白复合物稳定性中的作用。了解PTH和LA-PTH如何与PTHR结合并在特定亚细胞位置（内体）触发信号传导的结构细节和差异，可以深入了解分子和细胞过程，这可以为治疗提供新的机会。选择性靶向PTHR介导的内体Gs信号传导可能比全面靶向细胞表面信号传导提供更有效和选择性的治疗。

项目成果

Cdc42 activation couples fluid shear stress to apical endocytosis in proximal tubule cells.

• DOI: 10.14814/phy2.13460
• 发表时间: 2017-10
• 期刊: Physiological reports
• 影响因子: 2.5
• 作者: Bhattacharyya S;Jean-Alphonse FG;Raghavan V;McGarvey JC;Rbaibi Y;Vilardaga JP;Carattino MD;Weisz OA
• 通讯作者: Weisz OA

Reply to Ahluwalia et al.: Contributions of melatonin receptors are tissue-dependent.

回复 Ahluwalia 等人：褪黑激素受体的贡献是组织依赖性的。

• DOI: 10.1073/pnas.1800449115
• 发表时间: 2018
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Suofu,Yalikun;Carlisle,DianeL;Vilardaga,Jean-Pierre;Friedlander,RobertM
• 通讯作者: Friedlander,RobertM