Cellular and Molecular Mechanisms of FUS-related Amyotrophic Lateral Sclerosis

FUS相关肌萎缩侧索硬化症的细胞和分子机制

基本信息

  • 批准号:
    9230443
  • 负责人:
  • 金额:
    $ 32.92万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2014
  • 资助国家:
    美国
  • 起止时间:
    2014-03-01 至 2019-02-28
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Abstract Amyotrophic lateral sclerosis (ALS) is a late onset neurodegenerative disorder characterized by the loss of upper and lower motor neurons. FUS and TDP-43 are DNA/RNA binding proteins found to be mutated in both sporadic and familial forms of ALS. To investigate the pathogenesis of ALS caused by FUS/TLS mutations, we established a series of transgenic Drosophila lines that ectopically express human wild type and mutant FUS/TLS. Targeted expression of mutant, but not wild type FUS, in Drosophila eyes causes the formation of ubiquitinated aggregates and an external eye degenerative phenotype. Interestingly, ectopic expression of mutant FUS in motor neurons resulted in larval paralysis, pupal lethality, whereas wild type FUS expression had minimal effect. Mutant FUS localized to both the cytoplasm and nucleus, whereas wild type FUS localized only to the nucleus, suggesting that the cytoplasmic localization of FUS/TLS is required for toxicity. Furthermore, we found that deletion of the nuclear export signal strongly suppressed toxicity, suggesting that cytoplasmic localization is necessary for neurodegeneration. We found that expression of mutant FUS in the fly muscles leads to held-up wing phenotype, muscle degeneration and mitochondrial degeneration. We also found that mutating RNA binding residues of FUS strongly suppress mutant FUS toxicity in vivo. While doing an unbiased genetic screening, we discovered muscleblind as a novel modifier of mutant FUS toxicity. In our proposed studies, we will determine the role of mitochondrial functions in mediating FUS- related ALS and which functions of mitochondria are disrupted by the ALS causing mutations. We will systematically determine the role of RNA binding ability of FUS in causing ALS pathogenesis. We will also investigate if muscleblind regulates alternative splicing and sub- cellular distribution of FUS and contributes to the onset and progression of FUS-related ALS. Our proposed studies are expected to provide novel insights into the molecular mechanism of FUS-related ALS that would help in developing a therapeutic interventions for ALS for which currently no cure available.
描述(申请人提供):肌萎缩侧索硬化症(ALS)是一种以上下运动神经元丢失为特征的迟发性神经退行性疾病。FUS和TDP-43是DNA/RNA结合蛋白,在散发性和家族性ALS中均有突变。为了研究由FUS/TLS突变引起的ALS的发病机制,我们建立了一系列异位表达人野生型和突变型FUS/TLS的转基因果蝇系。在果蝇眼睛中靶向表达突变体,而不是野生型FUS,会导致泛素化聚集体的形成和外部眼睛退行性表型的形成。有趣的是,突变的FUS在运动神经元中的异位表达导致幼虫瘫痪,幼虫死亡,而野生型FUS的表达对幼虫的影响很小。突变型FUS定位于细胞质和细胞核,而野生型FUS仅定位于细胞核,表明FUS/TLS的细胞质定位是毒性所必需的。此外,我们发现核输出信号的缺失强烈抑制了毒性,这表明细胞质定位在神经退行性变中是必要的。我们发现,突变的FUS在果蝇肌肉中的表达会导致展翅表型、肌肉变性和线粒体退化。我们还发现,突变的FUS RNA结合残基在体内强烈抑制突变的FUS毒性。在进行无偏见的遗传筛选时,我们发现肌肉盲是突变FUS毒性的一种新的修饰物。在我们提出的研究中,我们将确定线粒体功能在介导FUS相关ALS中的作用,以及哪些线粒体功能被导致突变的ALS破坏。我们将系统地确定FUS的RNA结合能力在导致ALS发病中的作用。我们还将研究肌肉盲人是否调节FU和FU的选择性剪接和亚细胞分布。 有助于FUS相关肌萎缩侧索硬化症的发生和发展。我们拟议的研究有望为FUS相关ALS的分子机制提供新的见解,有助于开发目前尚无治愈方法的ALS的治疗干预措施。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Udai B Pandey其他文献

Udai B Pandey的其他文献

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{{ truncateString('Udai B Pandey', 18)}}的其他基金

Identifying the molecular mechanisms of GEMIN5 mutations in a novel cerebellar ataxia syndrome
鉴定新型小脑共济失调综合征中 GEMIN5 突变的分子机制
  • 批准号:
    10753403
  • 财政年份:
    2023
  • 资助金额:
    $ 32.92万
  • 项目类别:
Targeting epigenetic modifiers of androgen receptor activity and toxicity in SBMA
SBMA 中雄激素受体活性和毒性的表观遗传修饰因子
  • 批准号:
    9754495
  • 财政年份:
    2019
  • 资助金额:
    $ 32.92万
  • 项目类别:
Cellular and Molecular Mechanisms of FUS-related Amyotrophic Lateral Sclerosis
FUS相关肌萎缩侧索硬化症的细胞和分子机制
  • 批准号:
    9303039
  • 财政年份:
    2017
  • 资助金额:
    $ 32.92万
  • 项目类别:
Cellular and Molecular Mechanisms of FUS-related Amyotrophic Lateral Sclerosis
FUS相关肌萎缩侧索硬化症的细胞和分子机制
  • 批准号:
    9014564
  • 财政年份:
    2014
  • 资助金额:
    $ 32.92万
  • 项目类别:
Cellular and Molecular Mechanisms of FUS-related Amyotrophic Lateral Sclerosis
FUS相关肌萎缩侧索硬化症的细胞和分子机制
  • 批准号:
    8811504
  • 财政年份:
    2014
  • 资助金额:
    $ 32.92万
  • 项目类别:
Cellular and Molecular Mechanisms of FUS-related ALS/FTD
FUS相关ALS/FTD的细胞和分子机制
  • 批准号:
    10370356
  • 财政年份:
    2013
  • 资助金额:
    $ 32.92万
  • 项目类别:
Cellular and Molecular Mechanisms of FUS-related ALS/FTD
FUS相关ALS/FTD的细胞和分子机制
  • 批准号:
    9901635
  • 财政年份:
    2013
  • 资助金额:
    $ 32.92万
  • 项目类别:
Cellular and Molecular Mechanisms of FUS-related ALS/FTD
FUS相关ALS/FTD的细胞和分子机制
  • 批准号:
    9763021
  • 财政年份:
    2013
  • 资助金额:
    $ 32.92万
  • 项目类别:
Cellular and Molecular Mechanisms of FUS-related Amyotrophic Lateral Sclerosis
FUS相关肌萎缩侧索硬化症的细胞和分子机制
  • 批准号:
    8529133
  • 财政年份:
    2013
  • 资助金额:
    $ 32.92万
  • 项目类别:
Cellular and Molecular Mechanisms of FUS-related ALS/FTD
FUS相关ALS/FTD的细胞和分子机制
  • 批准号:
    10582582
  • 财政年份:
    2013
  • 资助金额:
    $ 32.92万
  • 项目类别:

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CAREER: Mechanotransduction, transcription, and alternative splicing in cell biology
职业:细胞生物学中的机械转导、转录和选择性剪接
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