Engineered Nanomaterial Synthesis, Characterization and Method Development Center for Nano-safety Research

项目总结/摘要 鉴于工业中使用的工程纳米材料（ENM）越来越广泛和多样化， 结合到消费品中，并用于医学成像和治疗应用， 了解它们的物理化学性质和生物相互作用之间的关系是至关重要的， 将有助于减少围绕ENM的仍然很大的风险不确定性。 针对这一RFA，我们建议建立“工程纳米材料合成， 纳米安全研究表征和方法开发协调中心。我们 拟建中心建立在现有学术研究的基础设施和跨学科经验之上 在ENM合成，表征和纳米安全研究领域的中心。这些国家的基础设施 合作中心，在过去的15年里发展，包括一个跨学科的研究小组， 教师，研究人员和学生，以及最先进的高通量合成ENM的平台， 包括金属和金属氧化物、尖端2D/3D ENM如CNT和石墨烯、纳米纤维素，以及 先进的纳米复合材料，加上创新的工具，以评估在生物系统中的ENM的命运， 统计和暴露评估工具，以及用于纳米毒理学的新型体外/体内平台。 本提案中概述的研究不仅限于及时和具有成本效益的综合， 为NHIR联合体提供参考ENM和协调服务的特征。我们的目标是 跨学科工作，分享新的想法，开发行业相关的参考ENM，并与 联合会开发多学科项目和标准化方法，以促进我们对 nano-EHS问题这项工作将通过一些高度相互关联的核心/目标来完成， 研究项目：1）ENM合成核心：它将提供一个广泛的阵列，良好的特点，工业- 涵盖传统和新兴高级2D/3D ENM的相关ENM; 2）ENM表征 核心：它将使用最先进的仪器和分析仪器提供完整的理化表征。 方法; 3）参考ENM库核心：参考ENM库的管理将包括 存储和分配ENM到财团，评估属性转换和纯度， 优化储存策略和QA/QC程序的时间和开发; 4）方法开发 核心：将开发用于毒理学的分散体制备和表征的标准化方法 研究，开发先进的剂量测定工具，用于ENM在生物介质（包括日冕）中的归宿和运输 表征方法，并开发用于纳米毒理学的化学和放射性示踪技术 社区，以跟踪细胞和动物模型中ENM的生物分布和易位; 5）施用 和研究协调核心：该核心将监督和协调所有中心研究者 和核心活动，并协调联合体成员之间的研究活动。

PROJECT SUMMARY/ABSTRACT Given the increasingly vast and varied array of engineered nanomaterials (ENMs) being utilized in industry, incorporated into consumer products, and employed in medical imaging and therapeutic applications, understanding the relationships between their physicochemical properties and biointeractions is critical and will help in reducing the still great risk uncertainty surrounding ENMs. In response to this RFA, we propose to establish the “Engineered Nanomaterial Synthesis, Characterization and Method Development Coordination Center for Nano-safety Research”. Our proposed Center builds upon the infrastructure and interdisciplinary experience of existing academic research centers in the fields of ENM synthesis, characterization and nano-safety research. The infrastructure in these collaborating centers, developed over the past 15 years, includes an inter-disciplinary research group of faculty, researchers and students, as well as state-of-the-art platforms for high throughput synthesis of ENMs, including metal and metal oxides, cutting edge 2D/3D ENMs such as CNTs and graphene, nanocellulose, and advanced nanocomposites, coupled with innovative tools to assess the fate of ENMs in biological systems, statistical and exposure assessment tools, and novel in vitro/in-vivo platforms for nanotoxicology. The research outlined in this proposal extends beyond timely and cost-effective synthesis and characterization of reference ENMs and coordination services for the NHIR consortium community. We aim to work across disciplines, share new ideas, develop industry-relevant reference ENMs, and work with the consortium to develop multidisciplinary projects and standardized methods to advance our understanding of nano-EHS issues. The work will be accomplished through a number of highly interconnected cores/aims and research projects: 1) ENM synthesis Core: It will provide an extensive array of well-characterized, industry- relevant ENMs that cover both conventional and emerging advanced 2D/3D ENMs; 2)ENM Characterization Core: it will provide complete physicochemical characterization using state of the art instruments and analytical methods; 3) Reference ENM repository Core: Management of the reference ENM repository will include storage and distribution of ENMs to the consortium, assessment of property transformations and purity over time and development of optimized storage strategies and QA/QC procedures; 4) Method Development Core: It will develop standardized methods for dispersion preparation and characterization for toxicology studies, develop advanced dosimetry tools for fate and transport of ENMs in biological media, including corona characterization methods, and develop chemical and radio-tracing techniques to be used by nanotoxicology community to track biodistribution and translocation of ENMs in cellular and animal models; 5) Administration and Research Coordination Core: This core will provide oversight and coordination of all center investigators and core activities and coordinate research activities among consortium members.