Miniature EPR sensor for on-site oximetry

用于现场血氧测定的微型 EPR 传感器

• 批准号: 9281723
• 负责人: PERIANNAN KUPPUSAMY
• 金额: $ 17.52万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2020-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9281723
• 关键词: Address; Animal Model; Area; Arts; Blood; Blood flow; Caliber; Cardiovascular Diseases; Cardiovascular system; Clinic; Clinical; Complex; Coupling; Crystallization; Data; Detection; Development; Devices; Diagnosis; Disease; Electron Spin Resonance Spectroscopy; Electronics; Environment; Future; Goals; Home environment; Hyperbaric Oxygenation; Hypoxia; Image; Implant; In Vitro; Knowledge; Length; Magnetic Resonance; Magnetic Resonance Imaging; Malignant Neoplasms; Measurement; Measures; Methods; Molecular; Monitor; Needle biopsy procedure; Oxygen; Oxygen saturation measurement; Pathology; Patients; Performance; Permeability; Physiologic pulse; Polymers; Procedures; Pulse Oximetry; Risk; Safety; Sampling; Sensitivity and Specificity; Shapes; Side; Signal Transduction; Silicones; Site; Skin; Specificity; Surface; System; Techniques; Technology; Temperature; Thinness; Time; Tissues; Treatment outcome; Work; Wound Healing; base; biomaterial compatibility; blood oxygen level dependent; clinically relevant; clinically significant; design; diagnostic assay; implanted sensor; improved; in vivo; innovation; interest; magnetic field; miniaturize; novel; outcome forecast; portability; programs; sensor; targeted treatment; tissue oxygenation; tool; treatment site; usability; wound

Abstract Knowledge of tissue oxygen level has enormous clinical significance in the diagnosis, prognosis, and treatment of several pathologies including cardiovascular disease, cancer, and wound healing. However, there is an unmet need for devices that can measure tissue oxygen in patients with a reasonable degree of accuracy, reliability, and robustness. Although a number of methods are considered applicable for oxygen measurements in patients, they lack the ability to make repeated measurements to monitor temporal changes during or post-therapy. Electron paramagnetic resonance (EPR)-based oximetry offers certain unique advantages over other methods, including high accuracy, high sensitivity to pO2 and high functional specificity. Unfortunately, the use of EPR-based techniques for clinical purposes still needs to address a number of fundamental issues, most notably the restrictions that arise from existing hardware. Most conventional EPR systems are large, bulky units with restrictive spacing between the magnet poles. Importantly, patients must be brought to the EPR facility for oxygen measurements. Hence, considerable changes in EPR hardware are necessary to make this technology more appealing and available by bedside or at treatment site from a clinical standpoint. The overall goal of this exploratory project is to develop a highly innovative miniature EPR device capable of repeated monitoring of deep tissue pO2 in patients. We propose to construct a unique self- contained compact EPR sensor system, called implantable oxygen sensor that can be used on site in the clinic. In contrast to the existing EPR system, which requires a large external magnet, our approach will use an ultra-small permanent magnet, which will be integrated within a resonator holding the oxygen-sensing probe. The all-in-one implantable unit will be of 2-mm diameter and 6-mm length and will be driven by a novel compact spectrometer, based on state-of-the-art field programmable gate array (FPGA) electronics. The specific aims of the two-year project are: (i) To construct a miniature EPR sensor using permanent magnets, loop-gap resonator, and oxygen- sensing crystal embedded in a silicone polymer attached to a compact, simple and affordable pulse EPR spectrometer; (ii) To validate the EPR sensor performance in vitro and in vivo using animal model. This unique device will enable continuous on-site and real-time monitoring of deep- tissue pO2 and may become a vital tool for clinical use to optimize various therapies for improving treatment outcomes.

抽象的 了解组织氧水平对于诊断、预后、 以及治疗多种疾病，包括心血管疾病、癌症和伤口 康复。然而，对能够测量患者组织氧的设备的需求尚未得到满足 具有合理程度的准确性、可靠性和稳健性。虽然方法很多 被认为适用于患者的氧气测量，但他们缺乏能力 重复测量以监测治疗期间或治疗后的时间变化。电子 与其他方法相比，基于顺磁共振 (EPR) 的血氧测定法具有某些独特的优势 方法，包括高精度、对 pO2 的高灵敏度和高功能特异性。 不幸的是，将基于 EPR 的技术用于临床目的仍然需要解决一个问题 许多基本问题，最明显的是现有硬件产生的限制。 大多数传统的 EPR 系统都是大型、笨重的装置，系统之间的间距有限。 磁极。重要的是，必须将患者带到 EPR 设施进行氧气测量。 因此，需要对 EPR 硬件进行相当大的改变，以使该技术更加有效。 从临床角度来看，它很有吸引力，并且可以在床边或治疗现场使用。总体目标 这个探索性项目的目的是开发一种高度创新的微型 EPR 装置，能够 反复监测患者深部组织 pO2。我们建议构建一个独特的自我 包含紧凑型 EPR 传感器系统，称为植入式氧传感器，可用于 诊所内的站点。与需要大型外部磁铁的现有 EPR 系统相比， 我们的方法将使用超小型永磁体，它将集成在谐振器内 握住氧传感探头。一体式植入装置的直径为 2 毫米， 长度为 6 毫米，由基于最先进领域的新型紧凑型光谱仪驱动 可编程门阵列 (FPGA) 电子产品。这个为期两年的项目的具体目标是： (i) 使用永磁体、环隙谐振器和氧传感器构建微型 EPR 传感器 嵌入硅聚合物中的传感晶体连接到一个紧凑、简单且价格实惠的 脉冲EPR光谱仪； (ii) 使用以下方法验证 EPR 传感器的体外和体内性能 动物模型。这种独特的设备将能够对深部进行连续的现场实时监控。 组织 pO2 可能成为临床使用的重要工具，用于优化各种疗法以改善 治疗结果。

项目成果

Compact electron spin resonance skin oximeter: Properties and initial clinical results.

紧凑型电子自旋共振皮肤血氧计：特性和初步临床结果。

• DOI: 10.1002/mrm.28595
• 发表时间: 2021
• 期刊: Magnetic resonance in medicine
• 影响因子: 3.3
• 作者: Cristea,David;Wolfson,Helen;Ahmad,Rizwan;Twig,Ygal;Kuppusamy,Periannan;Blank,Aharon
• 通讯作者: Blank,Aharon