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Fiber Inspired Neural Probes for the Multifunctional Dynamic Brain Mapping

用于多功能动态脑图绘制的纤维启发神经探针

基本信息

批准号：
9199380
负责人：
Polina O Anikeeva
金额：
$ 32.93万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-02-15 至 2019-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9199380
关键词：
Address Amygdaloid structure Anti-Inflammatory Agents Anti-inflammatory Appearance Behavior Behavioral Biological Assay Brain Brain Mapping Cells Chemicals Child Chronic Collaborations Complex Data Data Quality Decision Making Dependovirus Development Devices Dexamethasone Dimensions Drug Delivery Systems Electrodes Electrophysiology (science)Evolution Extinction (Psychology)Failure Farming environment Fiber Foreign Bodies Fright Future Generations Geometry Goals Histocompatibility Histologic Image Analysis Immunohistochemistry Implant Infusion procedures Institutes Ion Channel Knowledge Learning Letters Light Liquid substance Maps Measures Medial Medical Memory Metals Methods Microfluidics Modeling Modernization Mus Neurons Neurosciences Noise Optics Performance Pharmaceutical Preparations Pharmacology Physiology Polymers Population Prefrontal Cortex Process Research Research Institute Resolution Signal Transduction Silicon Structure Techniques Technology Time Tissue imaging Tissues Validation Viral Wild Type Mouse base cell cortex cell type conditioned fear density design electric impedance experimental study flexibility implantation in vivo light transmission mechanical properties model building multimodality nervous system disorder novel optogenetics public health relevance relating to nervous system response

项目摘要

DESCRIPTION (provided by applicant): The development of the high-resolution cell-specific map of the neural activity associated with a particular behavior presents one of the major challenges in modern neuroscience. This dynamic electrophysiological mapping is particularly difficult in behaviors with a strong temporal variability, such as learning or memory. It is furthr aggravated by the limited long-term stability of the existing high-density electrophysiological platforms and the inability to uniquely identify cell types during recording. This project strivesto develop novel multi-functional fiber-inspired neural probes (FINPs) for long- term simultaneous neural recording and optogenetic and pharmacological cell-type identification. Specifically, we will combine soft polymer-based materials with a fiber-inspired fabrication process to create a platform that seamlessly integrates hundreds of micrometer-size electrodes, waveguides and drug delivery channels, while minimizing the potential tissue response to chronic implantation. We will characterize our structures with respect to their tissue compatibility and long-term functionality in chronic experiments in collaboration with Dr. William Shain in Seattle Children's Research Institute (SCRI), who will share his knowledge of histological methods and image analysis. Furthermore, the utility of the proposed FINPs will be evaluated in a basic neuroscience study relevant to learning. FINPs will be used to measure the changes in activity (mPFC) neurons that project to the basolateral amygdala (BLA) as a previously learned fear response is extinguished. The combined behavioral and electrophysiological experiments will be performed in close collaboration with Dr. Alla Karpova at the Janelia Farm Research Campus (JFRC), who will contribute her expertise in mPFC physiology and cell identification techniques.