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Mutation Profile as Translatable Prognostic Biomarker of Uveal Melanoma

突变谱作为葡萄膜黑色素瘤的可翻译预后生物标志物

基本信息

批准号：
9116794
负责人：
ARUPA GANGULY
金额：
$ 17.4万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-08-01 至 2018-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9116794
关键词：
Adult American Joint Committee on Cancer BAP1 gene BRAF gene Biopsy Biopsy Specimen Categories Cells Chromosomes, Human, Pair 3 Classification Clinical Clinical Treatment Clinical Trials Clinical Trials Design Copy Number Polymorphism Custom Cutaneous Melanoma DNA Data Data Set Enrollment Frequencies Future GNAQ gene Genes Genome Genomics Guidelines Health Heterogeneity Incisional Biopsy Individual Integration Host Factors Investigation Knowledge Lead Lesion Malignant Neoplasms Medical Genetics Melanoma Cell Methods Monosomy Morbidity - disease rate Mutate Mutation Mutation Spectra Neoplasm Metastasis Outcome Pathway interactions Patient Recruitments Patients Primary Lesion Primary Neoplasm Prognostic Marker Resistance Risk SNP array Solid Somatic Mutation Specimen Structure Technology Testing Time Tissues Uveal Melanoma Work arm base cancer genome cell type clinically relevant cost effective exome exome sequencing genetic profiling improved inhibitor/antagonist malignant neoplasm of eye mutational status new therapeutic target next generation sequencing novel outcome forecast response therapy resistant tumor tumor heterogeneity whole genome

项目摘要

DESCRIPTION (provided by applicant): Uveal melanoma (UM) is highly resistant to current therapies. We hypothesize that precursors of metastatic UM cells are present at a small level in the primary tumor. The level of the mutation bearing cells is related to tumor heterogeneity implying the presence of multiple clones with different mutation spectrums. These cells evolve over time as a function of therapy and a variety of host factors. We will use the methods of next generation sequencing to take an unbiased look at the whole exome followed by cancer exome of multiple tumor genomes. The first objective is to define the somatic mutation profile of metastatic UM and compare the profiles of matched primary and metastatic lesions to see which mutations are selected in the metastatic lesions. In addition, identify mutations in known cancer associated genes, which may be present at very high (common) or low levels (rare), in the primary tumor and selected in metastatic UM. The second objective is to compare the mutation profiles of different sections of the same tumors selected based on the size and cell type profiles. This will detect different frequencies of mutations, if present, in the sections and defie intra-tumor heterogeneity. The third objective is to compare identified intra-tumor heterogeneity to other known genetic, clinical and histological features of the tumor as prognostic markers. The clinical relevance can be the knowledge that one biopsy of a large tumor does not provide enough information about the array of mutations present to design clinical trials or treatments based on the genetic profile of the tumor. In addition, the results may lead to novel therapeutic targets in UM.

描述（由申请人提供）：葡萄膜黑色素瘤（UM）对当前治疗具有高度耐药性。我们假设转移性UM细胞的前体在原发肿瘤中以小的水平存在。携带突变的细胞的水平与肿瘤异质性有关，这意味着存在具有不同突变谱的多个克隆。这些细胞随着时间的推移作为治疗和各种宿主因素的函数而进化。我们将使用下一代测序的方法，对多个肿瘤基因组的整个外显子组以及癌症外显子组进行无偏见的观察。第一个目的是确定转移性UM的体细胞突变谱，并比较匹配的原发性和转移性病变的谱，以了解在转移性病变中选择了哪些突变。此外，识别已知癌症相关基因中的突变，这些突变可能以非常高（常见）或低水平（罕见）存在于原发性肿瘤中，并在转移性UM中选择。第二个目的是比较基于大小和细胞类型谱选择的相同肿瘤的不同切片的突变谱。这将检测切片中不同频率的突变（如果存在），并确定肿瘤内异质性。第三个目标是将鉴别的肿瘤内异质性与肿瘤的其他已知遗传、临床和组织学特征作为预后标志物进行比较。临床相关性可以是大肿瘤的一次活检不能提供关于存在的突变阵列的足够信息以基于肿瘤的遗传谱设计临床试验或治疗的知识。此外，这些结果可能会导致新的治疗目标在UM。