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A Household Yeast Biosensor for Cholera

霍乱家用酵母生物传感器

基本信息

批准号：
9275835
负责人：
VIRGINIA W CORNISH
金额：
$ 70.93万
依托单位：
COLUMBIA UNIV NEW YORK MORNINGSIDE
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-05-15 至 2020-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9275835
关键词：
Africa African Anabolism Antibodies Benchmarking Binding Biosensor Bioterrorism Cause of Death Centers for Disease Control and Prevention (U.S.)Cholera Cholera Toxin Communities Country Coupling Detection Developing Countries Development Diagnostic Directed Molecular Evolution Disease Dried Yeast Engineering Epidemic Epitopes Equipment Evolution Eye Faculty Feces Feedback Fermentation Food Safety Formulation Freeze Drying Fungal Peptides G Protein-Coupled Receptor Signaling G-Protein-Coupled Receptors GTP-Binding Protein alpha Subunits, Gs Goals Grant Hand Health Hospitals Hour Household Household Products Human Hybrids Infection Laboratories Ligands Methods Monitor Noise Parents Peptides Pigments Plants Positioning Attribute Public Health Reaction Time Reagent Reporter Resources Risk Saccharomyces cerevisiae Scientist Series Signal Transduction Specificity System Technology Testing Tomatoes Translating Universities Validation Vibrio cholerae Visual Water Supply Yeasts base clinical practice cost design field study improved killings lycopene metabolic engineering pathogen product development prototype public health relevance receptor response surveillance network synthetic biology tool water sampling

项目摘要

DESCRIPTION (provided by applicant): Diarrheal diseases are one of the top ten causes of death in developing countries, with V. cholerae being one of the principal bacterial causative agents annually infecting an estimated ~4 million and killing ~120,000 people. Although an easily treatable disease, cholera continues to cause fatal epidemics because in practice clinical detection rarely occurs outside remote hospitals and environmental detection in water supplies remains challenging. The state-of-the- art for cholera diagnostics are purified antibodies used as dipstick tests; unfortunately dipstick tests are prohibitively expensive and unstable for use directly in local communities where cholera is endemic. A new transformative mode of detection will be needed to generate an extremely cheap and widely distributable system for continuous local surveillance of cholera. We sought to overcome current obstacles to local diagnostics by using existing synthetic biology components to craft a new class of cheap and simple diagnostic built around freeze-dried yeast. We avoid the need for expensive equipment and additional reagents by engineering yeast to produce the red tomato pigment lycopene in response to the cholera pathogen. We envision that engineered baker's yeast can be freeze-dried and then globally distributed to provide an inexpensive, safe, simple and reliable tool for monitoring of cholera in communities at risk for infection. If successful, this yeast platform will be easily redeployable for the detection of other pathogens.