Optogenetic control of protein assembly

摘要 技术和方法能够在精确的时间和时间操纵和探测蛋白质功能 对于实验生物学家来说，复杂细胞中的位置是强大的工具。这个项目的总体目标是 开发一套新的工具，允许由光触发的共价蛋白质连接，这可以用于 调节蛋白质活性，标记蛋白质，或组装新的蛋白质结构。在这个项目中，我们将结合 以植物为基础的光感受器，具有蛋白质-蛋白质连接的基因编码技术， 产生了新的工具，在这种工具中，两种蛋白质在接触闪光时可以永久地共价连接 光的力量。我们的工作将探索使用这些工具在接下来的较长时间内调节蛋白质的活性 打火机。我们预计这种工具将具有广泛的用途，能够对蛋白质功能进行时空控制， 蛋白质标签和大分子组装在一个完全遗传编码的模块中。 好了！

Summary Technologies and methods enabling the manipulation and probing of protein function at precise times and locations within complex cells are powerful tools for experimental biologists. The broad aims of this project are to develop a new suite of tools allowing covalent protein linkage triggered by light, which can be used to modulate protein activity, tag proteins, or assemble new protein architectures. In this project, we will combine light-responsive plant-based photoreceptors with genetically-encoded technologies for protein-protein ligation, resulting in new tools in which two proteins can be permanently and covalently linked upon exposure to a flash of light. Our work will explore use of these tools for modulating protein activity for extended periods following a light trigger. We anticipate such tools will have wide utility, enabling spatiotemporal control of protein function, protein tagging, and macromolecular assembly in a completely genetically-encoded module. !