Optogenetic control of protein assembly

蛋白质组装的光遗传学控制

• 批准号: 9434099
• 负责人: Chandra L Tucker
• 金额: $ 19.44万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-16 至 2019-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9434099
• 关键词: Architecture; Binding Proteins; Cell physiology; Cells; Chemicals; Clathrin; Clustered Regularly Interspaced Short Palindromic Repeats; Complement; Complex; DNA; Darkness; Dimerization; Directed Molecular Evolution; Disease; Endocytosis; Engineering; Enzymes; Exposure to; Fibronectins; Genetic Transcription; Goals; Health; Inositol; Kinetics; Label; Ligation; Light; Link; Location; Mammalian Cell; Mediating; Methods; Modification; Molecular; Molecular Probes; Monomeric GTP-Binding Proteins; Organelles; Pathway interactions; Peptides; Phosphoric Monoester Hydrolases; Photophobia; Photoreceptors; Physiologic pulse; Phytochrome; Plant Photoreceptors; Plants; Positioning Attribute; Process; Protein Engineering; Protein Fragment; Proteins; Reaction; Reagent; Recruitment Activity; Regulation; Research Personnel; Resolution; Rhodopsin; S-nitro-N-acetylpenicillamine; Signal Pathway; Specific qualifier value; Streptococcus pyogenes; System; Technology; Tetracyclines; Time; Tissues; Work; Yeasts; base; cryptochrome; cryptochrome 2; dimer; inhibitor/antagonist; innovation; macromolecular assembly; optogenetics; polypeptide; protein degradation; protein function; protein protein interaction; receptor; recombinase; screening; spatiotemporal; stem; temporal measurement; tool; transcription factor; ubiquitin-protein ligase

Summary Technologies and methods enabling the manipulation and probing of protein function at precise times and locations within complex cells are powerful tools for experimental biologists. The broad aims of this project are to develop a new suite of tools allowing covalent protein linkage triggered by light, which can be used to modulate protein activity, tag proteins, or assemble new protein architectures. In this project, we will combine light-responsive plant-based photoreceptors with genetically-encoded technologies for protein-protein ligation, resulting in new tools in which two proteins can be permanently and covalently linked upon exposure to a flash of light. Our work will explore use of these tools for modulating protein activity for extended periods following a light trigger. We anticipate such tools will have wide utility, enabling spatiotemporal control of protein function, protein tagging, and macromolecular assembly in a completely genetically-encoded module. !

总结 能够在精确的时间操纵和探测蛋白质功能的技术和方法， 复杂细胞内的定位是实验生物学家的有力工具。该项目的主要目标是 开发一套新的工具，允许由光引发的共价蛋白质连接， 调节蛋白质活性、标记蛋白质或组装新的蛋白质结构。在这个项目中，我们将联合收割机 基于植物的光感受器，具有用于蛋白质-蛋白质连接的遗传编码技术， 从而产生了新的工具，其中两种蛋白质可以在暴露于闪光时永久地共价连接 光明我们的工作将探索使用这些工具来调节蛋白质活性， 轻扳机我们预计这些工具将具有广泛的实用性，能够时空控制蛋白质功能， 蛋白质标记和完全遗传编码模块中的大分子组装。 !