Genetic analysis in an intractable gut microbe

摘要 栖息在脊椎动物肠道中的微生物对宿主有很大贡献 健康和疾病。肠道细菌相互作用的机制 彼此和它们的宿主了解得很少，因为大多数这些微生物 不受基因操纵的影响。我们建议将表型 化学诱变后的选择和基于群体的全基因组 测序以确定与粘蛋白降解相关的功能基因 粘液阿克曼氏菌，一种遗传上难以降解粘蛋白的肠道 与保护高脂肪诱导的代谢有关的微生物 疾病和炎症。拟议的工作将寻求识别分子 肠道定植机制和最佳益生功能。

ABSTRACT Microbes residing in the vertebrate intestinal tract contribute significantly to host health and disease. The mechanisms by which intestinal bacteria interact with each other and their host are poorly understood because most of these microbes are not amenable to genetic manipulation. We propose to combine phenotypic selection after chemical mutagenesis, and population-based whole genome sequencing to identify genes functionally associated with mucin degradation in the Akkermansia muciniphila, a genetically intractable mucin-degrading gut microbe that has been associated with protection from high-fat induced metabolic disorders and inflammation. The proposed work will seek to identify molecular mechanisms of gut colonization and optimal pro-biotic functions.