BSLII Stationed Zeiss 880 Confocal Microscope with Airyscan

这项提案要求为NIH资助的一组人购买激光扫描共聚焦显微镜 来自加州大学圣克鲁斯分校几个部门的调查人员。建议的蔡司880 配备Airycan的系统将取代我们目前购买的Zeiss Pascal共焦显微镜 通过NIH的SIG机制并于2004年安装。蔡司LSM 880是一款灵活的模块化共焦 用于高端荧光成像的激光扫描显微镜。LSM 880是一款出色的 核心设施，因为它非常适合我们的用户的研究目标，它易于使用，并提供成本- 有效解决我们的研究挑战。建议的仪器对于美国国立卫生研究院资助的生物医学是必不可少的 在加州大学圣克鲁斯分校进行的研究。主要的用户群体是由多个NIH项目资助的。这些是 国家过敏和传染病研究所(NIAID)，国家普通医学研究所 国家眼科研究所(NIGMS)、国家眼科研究所(NEI)。他们试图将共焦显微镜的力量带到 细菌致病机制及生物被膜形成机制、细菌在动物体内运动的研究 免疫耐受期间的器官和免疫细胞相互作用，细胞周期研究，退行性 眼科疾病和视网膜可塑性。该显微镜系统将成为加州大学洛杉矶分校生活中不可或缺的一部分 科学显微镜中心，并将设在我们生物医学科学内的BSLII指定空间 大楼。此放置是处理第2组风险样本所必需的。该设施对所有由美国国立卫生研究院资助的 调查人员；因此，该设备将加强和支持本校园的生物医学研究，同时 为我们的研究生和博士后研究员提供高级培训，从而帮助 造就下一代生物医学研究人员。

This proposal requests funds to purchase a laser scanning confocal microscope for a group of NIH-funded investigators from several departments at the University of California at Santa Cruz. The proposed Zeiss 880 system with Airyscan will replace our current Zeiss PASCAL confocal microscope, which was purchased through NIH's SIG mechanism and installed in 2004. The Zeiss LSM 880 is a flexible and modular confocal laser scanning microscope for high end fluorescence imaging. The LSM 880 is an outstanding choice for a core facility as it is well suited for the research objectives of our users, it is easy to use and provides a cost- effective solution to our research challenges. The proposed instrument is essential for NIH-funded biomedical research taking place at UC Santa Cruz. The major user group is funded by multiple NIH programs. These are the National Institute of Allergy and Infectious Diseases (NIAID), The National Institute of General Medical Sciences (NIGMS), National Eye Institute (NEI). They seek to bring the power of confocal microscopy to studies of bacterial pathogenesis and mechanism of biofilm formation, bacterial movement within animal organs, and immune cell interactions during immunological tolerance, investigation of cell cycle, degenerative eye diseases and retinal plasticity. This microscope system will become an integral part of UCSC's Life Sciences Microscopy Center, and will located in a BSLII designated space within our Biomedical Sciences building. This placement is required for work on Risk Group 2 samples. The facility is open to all NIH-funded investigators; thus, this equipment will enhance and sustain biomedical research on this campus, while providing advanced training to our graduate students and postdoctoral research fellows, thereby helping to create the next generation of biomedical investigators.