BSLII Stationed Zeiss 880 Confocal Microscope with Airyscan

该提案要求资金为NIH资助的一组激光扫描共聚焦显微镜购买 来自圣克鲁斯的加州大学几个系的调查人员。蔡司880 Airyscan系统将取代我们目前购买的Zeiss PASCAL共聚焦显微镜， 通过NIH的SIG机制，并于2004年安装。蔡司LSM 880是一款灵活的模块化共焦显微镜， 用于高端荧光成像的激光扫描显微镜。LSM 880是 核心设施，因为它是非常适合我们的用户的研究目标，它很容易使用，并提供了一个成本- 有效解决我们的研究挑战。拟议的仪器是必不可少的NIH资助的生物医学 在加州大学圣克鲁斯进行的研究。主要用户群由多个NIH项目资助。这些是 国家过敏和传染病研究所（NIAID），国家普通医学研究所 国家眼科研究所（NEI）。他们试图将共聚焦显微镜的力量， 细菌致病性、生物被膜形成机制、细菌在动物体内运动的研究 器官，免疫耐受过程中免疫细胞的相互作用，细胞周期的研究， 眼科疾病和视网膜可塑性。这个显微镜系统将成为UCSC生命的一个组成部分 科学显微镜中心，并将设在BSLII指定的空间内，我们的生物医学科学 建设风险组2样本的工作需要该位置。该设施向所有NIH资助的 因此，这些设备将加强和维持这个校园的生物医学研究， 为我们的研究生和博士后研究员提供高级培训，从而帮助 培养下一代的生物医学研究者。

This proposal requests funds to purchase a laser scanning confocal microscope for a group of NIH-funded investigators from several departments at the University of California at Santa Cruz. The proposed Zeiss 880 system with Airyscan will replace our current Zeiss PASCAL confocal microscope, which was purchased through NIH's SIG mechanism and installed in 2004. The Zeiss LSM 880 is a flexible and modular confocal laser scanning microscope for high end fluorescence imaging. The LSM 880 is an outstanding choice for a core facility as it is well suited for the research objectives of our users, it is easy to use and provides a cost- effective solution to our research challenges. The proposed instrument is essential for NIH-funded biomedical research taking place at UC Santa Cruz. The major user group is funded by multiple NIH programs. These are the National Institute of Allergy and Infectious Diseases (NIAID), The National Institute of General Medical Sciences (NIGMS), National Eye Institute (NEI). They seek to bring the power of confocal microscopy to studies of bacterial pathogenesis and mechanism of biofilm formation, bacterial movement within animal organs, and immune cell interactions during immunological tolerance, investigation of cell cycle, degenerative eye diseases and retinal plasticity. This microscope system will become an integral part of UCSC's Life Sciences Microscopy Center, and will located in a BSLII designated space within our Biomedical Sciences building. This placement is required for work on Risk Group 2 samples. The facility is open to all NIH-funded investigators; thus, this equipment will enhance and sustain biomedical research on this campus, while providing advanced training to our graduate students and postdoctoral research fellows, thereby helping to create the next generation of biomedical investigators.