A Comprehensive 5K plus Glycan Microarray

项目总结/摘要 越来越需要高通量分析平台来研究细胞-表面聚糖的相互作用， 聚糖结合蛋白。我们在这里建议开发一个5 K+聚糖阵列，以促进功能性糖组学 research.开发全面的聚糖阵列的两个障碍是（1）缺乏敏感的聚糖 能够实现多价结合事件的阵列基底，和（2）缺乏聚糖的综合聚糖文库 代表整个糖组的结构。为了解决这一挑战，Z Biotech将与Chemily合作 Glycoscience为市场提供最全面的聚糖阵列和聚糖子阵列。Z Biotech 发明了一种稳健的微阵列基底，其具有在固体表面上以多价显示聚糖的优点。 Chemily是一家领先的糖科学公司，生产复合聚糖，糖核苷酸和 聚糖相关酶。所提出的研究策略得到了初步实验的支持。 两家公司合作调查。在第一阶段，我们的具体目标是（1）收集 和合成8类1.2K+聚糖（子阵列），以及（2）1.2K+聚糖阵列制造和 QC/QA评价。最后，聚糖阵列将被提供给大型聚糖阵列筛选中心，用于进一步的研究。 评价这些都是SBIR计划框架内的现实目标，所得聚糖阵列产品将 对糖生物学研究、聚糖相关生物标志物表征和新药开发产生广泛影响 发展

Project Summary / Abstract There is growing need of high-throughput analytical platforms to study interactions of cell-surface glycans and glycan-binding proteins. We propose here to develop a 5K+ glycan array to facilitate functional glycomics research. Two hurdles for development of a comprehensive glycan array are (1) lack of a sensitive glycan array substrate enabling multivalent binding events, and (2) lack of a comprehensive glycan library of glycan structures representing the whole glycomes. To solve this challenge, Z Biotech will team with Chemily Glycoscience to deliver the most comprehensive glycan array and glycan sub-arrays to market. Z Biotech invented a robust microarray substrate having the advantage to display glycans on solid surface in multivalent form, and Chemily is a leading glycoscience company in production of complex glycans, sugar nucleotides and glycan-related enzymes. The proposed research strategy has been supported by a preliminary experimental investigation under collaboration between the two companies. In Phase I, our specific aims are (1) collection and synthesis of 1.2K+ glycans in 8 categories (sub-arrays), and (2) 1.2K+ glycan array manufacturing and QC/QA evaluation. Finally, the glycan arrays will be provided to large glycan array screening centers for further evaluation. These are realistic goals within a SBIR program frame and the resultant glycan array products will have a broad impact on glycobiology research, glycan-related biomarker characterization and new drug development.