A Comprehensive 5K plus Glycan Microarray

项目摘要/摘要 对高通量分析平台的需求日益增长，以研究细胞-表面多糖和 糖链结合蛋白。我们建议开发一种5K糖链阵列以促进功能糖组分 研究。开发全面的多聚糖阵列的两个障碍是(1)缺乏敏感的多聚糖 能够发生多价结合事件的阵列底物，以及(2)缺乏全面的葡聚糖库 代表整个糖类的结构。为了解决这一挑战，Z Biotech将与Chemly合作 Glycocience将向市场提供最全面的糖链阵列和糖链亚阵列。Z Biotech 发明了一种坚固的微阵列基板，具有在固体表面以多价显示多糖的优点 化学公司是一家领先的糖科学公司，生产复杂的多糖、糖核苷酸和 与糖链相关的酶。所提出的研究策略已得到初步实验的支持 调查正在两家公司的合作下进行。在第一阶段，我们的具体目标是(1)收集 和(2)1.2K多聚糖阵列的制造和 QC/QA评估。最后，将所述多聚糖阵列提供给大型多聚糖阵列筛选中心进行进一步筛选 评估。这些都是SBIR计划框架内的现实目标，所得的多糖阵列产品将 对糖生物学研究、与糖相关的生物标志物表征和新药产生广泛影响 发展。

Project Summary / Abstract There is growing need of high-throughput analytical platforms to study interactions of cell-surface glycans and glycan-binding proteins. We propose here to develop a 5K+ glycan array to facilitate functional glycomics research. Two hurdles for development of a comprehensive glycan array are (1) lack of a sensitive glycan array substrate enabling multivalent binding events, and (2) lack of a comprehensive glycan library of glycan structures representing the whole glycomes. To solve this challenge, Z Biotech will team with Chemily Glycoscience to deliver the most comprehensive glycan array and glycan sub-arrays to market. Z Biotech invented a robust microarray substrate having the advantage to display glycans on solid surface in multivalent form, and Chemily is a leading glycoscience company in production of complex glycans, sugar nucleotides and glycan-related enzymes. The proposed research strategy has been supported by a preliminary experimental investigation under collaboration between the two companies. In Phase I, our specific aims are (1) collection and synthesis of 1.2K+ glycans in 8 categories (sub-arrays), and (2) 1.2K+ glycan array manufacturing and QC/QA evaluation. Finally, the glycan arrays will be provided to large glycan array screening centers for further evaluation. These are realistic goals within a SBIR program frame and the resultant glycan array products will have a broad impact on glycobiology research, glycan-related biomarker characterization and new drug development.