A Comprehensive 5K plus Glycan Microarray

综合 5K plus 聚糖微阵列

• 批准号: 9407238
• 负责人: Jian Zhang
• 金额: $ 15.49万
• 依托单位: Z BIOTECH, LLC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9407238
• 关键词: Achievement; Amino Acids; Animals; Antibodies; B-Lymphocytes; Binding; Binding Proteins; Biological; Biological Markers; Biotechnology; Carbohydrates; Categories; Cell Communication; Cell membrane; Cell surface; Characteristics; Chemistry; Collaborations; Collection; Complex; Data; Development; Engineering; Enzymes; Evaluation; Event; Family; GAG Gene; Gangliosides; Generations; Glycobiology; Goals; Human; Immobilization; Industrialization; Investigation; Lectin; Libraries; Lipids; Liquid substance; Mediating; Mission; Natural Products; Noise; Oligosaccharides; Performance; Phase; Plants; Polysaccharides; Production; Proteins; Research; Research Personnel; Resources; S-1 Antimetabolite agent; S-7; Signal Transduction; Small Business Innovation Research Grant; Solid; Specificity; Structure; Surface; System; Technology; Therapeutic; Vaccines; Validation; Variant; Work; base; carbohydrate receptor; commercial application; comparative; drug development; innovation; macromolecule; molecular recognition; new technology; novel therapeutics; programs; receptor; screening; success; sugar nucleotide; tool

Project Summary / Abstract There is growing need of high-throughput analytical platforms to study interactions of cell-surface glycans and glycan-binding proteins. We propose here to develop a 5K+ glycan array to facilitate functional glycomics research. Two hurdles for development of a comprehensive glycan array are (1) lack of a sensitive glycan array substrate enabling multivalent binding events, and (2) lack of a comprehensive glycan library of glycan structures representing the whole glycomes. To solve this challenge, Z Biotech will team with Chemily Glycoscience to deliver the most comprehensive glycan array and glycan sub-arrays to market. Z Biotech invented a robust microarray substrate having the advantage to display glycans on solid surface in multivalent form, and Chemily is a leading glycoscience company in production of complex glycans, sugar nucleotides and glycan-related enzymes. The proposed research strategy has been supported by a preliminary experimental investigation under collaboration between the two companies. In Phase I, our specific aims are (1) collection and synthesis of 1.2K+ glycans in 8 categories (sub-arrays), and (2) 1.2K+ glycan array manufacturing and QC/QA evaluation. Finally, the glycan arrays will be provided to large glycan array screening centers for further evaluation. These are realistic goals within a SBIR program frame and the resultant glycan array products will have a broad impact on glycobiology research, glycan-related biomarker characterization and new drug development.

项目摘要 /摘要 越来越需要高通量分析平台来研究细胞表面聚糖和 聚糖结合蛋白。我们在这里建议开发一个5K+聚糖阵列，以促进功能性糖化 研究。开发全面的聚糖阵列的两个障碍是（1）缺乏敏感的聚糖 阵列底物实现多价绑定事件，以及（2）缺乏全面的聚糖库 代表整个糖果的结构。为了解决这一挑战，Z Biotech将与化学合作 聚糖可以将最全面的聚糖阵列和聚糖子阵列推向市场。 Z Biotech 发明了一个强大的微阵列基材，具有在多价中显示在固体表面上的聚糖的优势 形式，化学是一家领先的聚糖公司，用于生产复杂的聚糖，糖核苷酸和 与聚糖有关的酶。提出的研究策略得到了初步实验的支持 两家公司之间的协作调查。在第一阶段，我们的具体目标是（1）收集 并合成8个类别（子阵列）的1.2k+ Glycans，以及（2）1.2K+ Glycan阵列制造和 QC/QA评估。最后，将向大型聚糖阵列筛选中心提供聚糖阵列以便进一步 评估。这些是SBIR程序框架中的现实目标，由此产生的聚糖阵列产品将 对糖生物学研究，与聚糖相关的生物标志物和新药有广泛的影响 发展。