A Comprehensive 5K plus Glycan Microarray

综合 5K plus 聚糖微阵列

• 批准号: 9407238
• 负责人: Jian Zhang
• 金额: $ 15.49万
• 依托单位: Z BIOTECH, LLC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9407238
• 关键词: Achievement; Amino Acids; Animals; Antibodies; B-Lymphocytes; Binding; Binding Proteins; Biological; Biological Markers; Biotechnology; Carbohydrates; Categories; Cell Communication; Cell membrane; Cell surface; Characteristics; Chemistry; Collaborations; Collection; Complex; Data; Development; Engineering; Enzymes; Evaluation; Event; Family; GAG Gene; Gangliosides; Generations; Glycobiology; Goals; Human; Immobilization; Industrialization; Investigation; Lectin; Libraries; Lipids; Liquid substance; Mediating; Mission; Natural Products; Noise; Oligosaccharides; Performance; Phase; Plants; Polysaccharides; Production; Proteins; Research; Research Personnel; Resources; S-1 Antimetabolite agent; S-7; Signal Transduction; Small Business Innovation Research Grant; Solid; Specificity; Structure; Surface; System; Technology; Therapeutic; Vaccines; Validation; Variant; Work; base; carbohydrate receptor; commercial application; comparative; drug development; innovation; macromolecule; molecular recognition; new technology; novel therapeutics; programs; receptor; screening; success; sugar nucleotide; tool

Project Summary / Abstract There is growing need of high-throughput analytical platforms to study interactions of cell-surface glycans and glycan-binding proteins. We propose here to develop a 5K+ glycan array to facilitate functional glycomics research. Two hurdles for development of a comprehensive glycan array are (1) lack of a sensitive glycan array substrate enabling multivalent binding events, and (2) lack of a comprehensive glycan library of glycan structures representing the whole glycomes. To solve this challenge, Z Biotech will team with Chemily Glycoscience to deliver the most comprehensive glycan array and glycan sub-arrays to market. Z Biotech invented a robust microarray substrate having the advantage to display glycans on solid surface in multivalent form, and Chemily is a leading glycoscience company in production of complex glycans, sugar nucleotides and glycan-related enzymes. The proposed research strategy has been supported by a preliminary experimental investigation under collaboration between the two companies. In Phase I, our specific aims are (1) collection and synthesis of 1.2K+ glycans in 8 categories (sub-arrays), and (2) 1.2K+ glycan array manufacturing and QC/QA evaluation. Finally, the glycan arrays will be provided to large glycan array screening centers for further evaluation. These are realistic goals within a SBIR program frame and the resultant glycan array products will have a broad impact on glycobiology research, glycan-related biomarker characterization and new drug development.

项目摘要/摘要 对高通量分析平台的需求日益增长，以研究细胞-表面多糖和 糖链结合蛋白。我们建议开发5K+多聚糖阵列以促进功能糖组分 研究。开发全面的多聚糖阵列的两个障碍是(1)缺乏敏感的多聚糖 能够发生多价结合事件的阵列底物，以及(2)缺乏全面的葡聚糖库 代表整个糖类的结构。为了解决这一挑战，Z Biotech将与Chemly合作 Glycocience将向市场提供最全面的糖链阵列和糖链亚阵列。Z Biotech 发明了一种坚固的微阵列基板，具有在固体表面以多价显示多糖的优点 化学公司是一家领先的糖科学公司，生产复杂的多糖、糖核苷酸和 与糖链相关的酶。所提出的研究策略已得到初步实验的支持 调查正在两家公司的合作下进行。在第一阶段，我们的具体目标是(1)收集 和(2)1.2K+多聚糖阵列的制备和 QC/QA评估。最后，将所述多聚糖阵列提供给大型多聚糖阵列筛选中心进行进一步筛选 评估。这些都是SBIR计划框架内的现实目标，所得的多糖阵列产品将 对糖生物学研究、与糖相关的生物标志物表征和新药产生广泛影响 发展。