The Influence of Capsule Composition on Lens Biology

胶囊成分对晶状体生物学的影响

• 批准号: 9242641
• 负责人: MELINDA K DUNCAN
• 金额: $ 39万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-12-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9242641
• 关键词: Adverse effects; Basement membrane; Biology; Cataract; Cataract Extraction; Cells; Cicatrix; Communication; Data; Development; Embryo; Epithelial; Epithelial Cells; Epithelium; Excision; Extracapsular; Extracellular Matrix; Extracellular Matrix Proteins; Eye; Fibronectins; Gene Expression; Grant; Integrins; Lens Fiber; Lens development; Ligands; Mediating; Molecular; Molecular Target; Myofibroblast; Operative Surgical Procedures; Pathogenesis; Pathway interactions; Patient-Focused Outcomes; Phenotype; Play; Prevention; Process; Proteins; Regulation; Role; Signal Transduction; Surface; Testing; Tissues; Vesicle; Visual; Work; capsule; cell behavior; epithelial to mesenchymal transition; fiber cell; insight; lens; lens capsule; premature; prevent; public health relevance; transdifferentiation

DESCRIPTION (provided by applicant): The lens capsule is a thickened basement membrane surrounding the lens which provides essential structural support for lens cells and mediates connection between the lens and the remainder of the eye. Lens capsule components also serve as survival, proliferation and differentiation signals for the attached lens cells although te relative contribution of each extracellular matrix protein found in the lens capsule to lens cell behavior is mostly uncharacterized.. Integrins are major molecules mediating the communication between cells and their extracellular matrix. During the prior grant cycle we discovered that various integrins play essential roles in lens development as well as the pathogenesis of posterior capsular opacification (PCO), the major side effect of surgical cataract removal. However, the mechanisms by which integrins control lens development and the pathogenesis of PCO is not well understood. This application proposes to investigate the molecular mechanisms by which integrins control the fate decisions which regulate transitions from lens epithelial cells to fiber cells during development and "pearl type" PCO as well as transitions between epithelial cells and myofibroblasts during the process of fibrotic PCO development.

描述（由申请人提供）：透镜囊是围绕透镜的增厚基底膜，为透镜细胞提供必要的结构支撑，并介导透镜和眼睛其余部分之间的连接。透镜囊组分还充当附着的透镜细胞的存活、增殖和分化信号，尽管在透镜囊中发现的每种细胞外基质蛋白对透镜细胞行为的相对贡献大多未被表征。整合素是介导细胞与其细胞外基质之间通讯的主要分子。在之前的研究周期中，我们发现各种整合素在透镜发育以及后囊混浊（PCO）的发病机制中起着重要作用，PCO是白内障手术切除的主要副作用。然而，整合素控制透镜发育和PCO发病机制的机制还不清楚。本申请提出研究整合素控制命运决定的分子机制，所述命运决定调节发育期间从透镜上皮细胞到纤维细胞的转变和“珍珠型”PCO以及纤维化PCO发育过程中上皮细胞和肌成纤维细胞之间的转变。