Acquisition of a Zeiss LSM710 Confocal Microscope

购买蔡司 LSM710 共焦显微镜

• 批准号: 7792780
• 负责人: MELINDA K DUNCAN
• 金额: $ 49.44万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792780
• 关键词: Animals; Arthritis; Biological Sciences; Biomedical Research; Cataract; Cells; Communities; Consult; Contract Services; Core Facility; Delaware; Development; Disease; Engineering; Ensure; Equipment; Foundations; Funding; Housing; Image; Imaging Device; Imaging Techniques; Life; Longevity; Maintenance; Malignant Neoplasms; Manufacturer Name; Microscope; Noise; Osteoporosis; Prosthesis; Request for Applications; Research; Research Activity; Research Personnel; Research Project Grants; Resolution; Resources; Schedule; Schools; Spectrum Analysis; Thrombosis; Tissue Engineering; Tissues; Training; United States National Institutes of Health; Universities; Work; angiogenesis; animal facility; instrument; meetings; repaired; tissue culture

DESCRIPTION (provided by applicant): The University of Delaware (UD) is classified as a "very high research activity research university" by the Carnegie Foundation. Much of this research activity focuses on diverse fields of biomedical research, particularly in the Department of Biological Sciences and the School of Engineering and relies heavily on advanced tools for the imaging of both tissues and cells. In order to maximize the utilization of these critical instruments, UD established a multi-user imaging core facility which is responsible for equipment scheduling and maintenance, the training of users and advanced technical consulting to develop new imaging techniques. One of the first instruments acquired by this facility (over a decade ago) was a LSM510 confocal microscope which is housed in the Department of Biological Sciences due to the proximity of its major users, tissue culture facilities and the UD animal facility. This instrument is heavily used by investigators across UD, however, it is reaching the end of its lifespan and its manufacturer will no longer provide service contracts and guarantee availability of the full range of parts for its maintenance after summer 2009. This application requests funds to replace this obsolete instrument which no longer meets the full needs of our user community with an upright LSM710 confocal microscope which will be located in the microscope suite in the Department of Biological Sciences and administered by the UD core imaging facility staff. This will ensure access to a working confocal microscope on the UD main campus to all investigators who utilize the UD core imaging facility. This is a critical resource for numerous NIH funded research projects at UD investigating diverse diseases including cataracts, osteoporosis, arthritis, cancer, angiogenesis and thrombosis and will also contribute to the development of tissue prostheses to repair or replace damaged tissues. Furthermore, this microscope will enhance the biomedical research capabilities at UD since it has capabilities absent from the prior microscope including increased sensitivity, the ability to clearly image low magnification images at high pixel resolution, the ability to perform raster image correlation spectroscopy at high sensitivity and low noise, and as our only confocal with an upright configuration will be ideal to meet our growing needs to conduct fluorescent imaging of whole animals, dissected tissues and engineered living tissue prostheses.

描述（由申请人提供）：特拉华州大学（UD）被卡内基基金会列为“非常高的研究活动研究型大学”。 这项研究活动的大部分集中在生物医学研究的各个领域，特别是生物科学系和工程学院，并且严重依赖于组织和细胞成像的先进工具。 为了最大限度地利用这些关键仪器，UD建立了一个多用户成像核心设施，负责设备调度和维护、用户培训和先进的技术咨询，以开发新的成像技术。 该设施（十多年前）获得的第一批仪器之一是LSM 510共聚焦显微镜，由于其主要用户，组织培养设施和UD动物设施的接近，该显微镜位于生物科学系。 该仪器被整个UD的调查人员大量使用，但其使用寿命即将结束，其制造商将不再提供服务合同，并保证在2009年夏季之后提供全套部件进行维护。 本申请要求资金，以取代这一过时的仪器，不再满足我们的用户社区的全部需求与直立LSM 710共聚焦显微镜，将位于显微镜套件在生物科学系和UD核心成像设施的工作人员管理。 这将确保使用UD核心成像设施的所有研究人员都能使用UD主校区的工作共聚焦显微镜。 这是众多NIH资助的研究项目在UD调查各种疾病，包括白内障，骨质疏松症，关节炎，癌症，血管生成和血栓形成的关键资源，也将有助于组织假体的开发，以修复或替换受损组织。 此外，这种显微镜将提高UD的生物医学研究能力，因为它具有现有显微镜所没有的能力，包括增加的灵敏度，以高像素分辨率清晰成像低放大率图像的能力，以高灵敏度和低噪声执行光栅图像相关光谱的能力，并且作为我们唯一的具有直立配置的共焦，将是满足我们对整个动物、解剖组织和工程活组织假体进行荧光成像的日益增长的需求的理想选择。