Acquisition of a Zeiss LSM710 Confocal Microscope

购买蔡司 LSM710 共焦显微镜

• 批准号: 7792780
• 负责人: MELINDA K DUNCAN
• 金额: $ 49.44万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792780
• 关键词: Animals; Arthritis; Biological Sciences; Biomedical Research; Cataract; Cells; Communities; Consult; Contract Services; Core Facility; Delaware; Development; Disease; Engineering; Ensure; Equipment; Foundations; Funding; Housing; Image; Imaging Device; Imaging Techniques; Life; Longevity; Maintenance; Malignant Neoplasms; Manufacturer Name; Microscope; Noise; Osteoporosis; Prosthesis; Request for Applications; Research; Research Activity; Research Personnel; Research Project Grants; Resolution; Resources; Schedule; Schools; Spectrum Analysis; Thrombosis; Tissue Engineering; Tissues; Training; United States National Institutes of Health; Universities; Work; angiogenesis; animal facility; instrument; meetings; repaired; tissue culture

DESCRIPTION (provided by applicant): The University of Delaware (UD) is classified as a "very high research activity research university" by the Carnegie Foundation. Much of this research activity focuses on diverse fields of biomedical research, particularly in the Department of Biological Sciences and the School of Engineering and relies heavily on advanced tools for the imaging of both tissues and cells. In order to maximize the utilization of these critical instruments, UD established a multi-user imaging core facility which is responsible for equipment scheduling and maintenance, the training of users and advanced technical consulting to develop new imaging techniques. One of the first instruments acquired by this facility (over a decade ago) was a LSM510 confocal microscope which is housed in the Department of Biological Sciences due to the proximity of its major users, tissue culture facilities and the UD animal facility. This instrument is heavily used by investigators across UD, however, it is reaching the end of its lifespan and its manufacturer will no longer provide service contracts and guarantee availability of the full range of parts for its maintenance after summer 2009. This application requests funds to replace this obsolete instrument which no longer meets the full needs of our user community with an upright LSM710 confocal microscope which will be located in the microscope suite in the Department of Biological Sciences and administered by the UD core imaging facility staff. This will ensure access to a working confocal microscope on the UD main campus to all investigators who utilize the UD core imaging facility. This is a critical resource for numerous NIH funded research projects at UD investigating diverse diseases including cataracts, osteoporosis, arthritis, cancer, angiogenesis and thrombosis and will also contribute to the development of tissue prostheses to repair or replace damaged tissues. Furthermore, this microscope will enhance the biomedical research capabilities at UD since it has capabilities absent from the prior microscope including increased sensitivity, the ability to clearly image low magnification images at high pixel resolution, the ability to perform raster image correlation spectroscopy at high sensitivity and low noise, and as our only confocal with an upright configuration will be ideal to meet our growing needs to conduct fluorescent imaging of whole animals, dissected tissues and engineered living tissue prostheses.

描述（由申请人提供）：特拉华大学 (UD) 被卡内基基金会归类为“研究活动非常高的研究型大学”。 这项研究活动大部分集中在生物医学研究的不同领域，特别是生物科学系和工程学院，并在很大程度上依赖于组织和细胞成像的先进工具。 为了最大限度地利用这些关键仪器，UD建立了一个多用户成像核心设施，负责设备调度和维护、用户培训以及开发新成像技术的先进技术咨询。 该设施（十多年前）购买的第一批仪器之一是 LSM510 共焦显微镜，由于靠近其主要用户、组织培养设施和 UD 动物设施，该显微镜位于生物科学系。 该仪器在 UD 的研究人员中大量使用，但是，它的使用寿命即将结束，其制造商将不再提供服务合同并保证 2009 年夏季之后提供全系列零件的可用性。该申请需要资金来更换这种过时的仪器，该仪器不再能满足我们用户社区的全部需求，使用直立式 LSM710 共焦显微镜，该显微镜将位于生物科学系的显微镜套件中，并且 由 UD 核心成像设施工作人员管理。 这将确保所有使用 UD 核心成像设施的研究人员都能使用 UD 主校区的共焦显微镜。 这是美国国立卫生研究院 (NIH) 资助的特拉华大学研究项目的重要资源，这些项目研究白内障、骨质疏松症、关节炎、癌症、血管生成和血栓形成等多种疾病，还将有助于开发修复或替换受损组织的组织假体。 此外，该显微镜将增强 UD 的生物医学研究能力，因为它具有以前显微镜所没有的功能，包括更高的灵敏度、以高像素分辨率清晰地成像低放大倍率图像的能力、以高灵敏度和低噪声执行光栅图像相关光谱的能力，并且作为我们唯一具有直立配置的共焦，将非常适合满足我们对整个动物进行荧光成像的不断增长的需求，解剖 组织和工程活体组织假体。