权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Genetic tools for visualization of co-transmission in identified neurons

用于可视化已识别神经元中的共传递的遗传工具

基本信息

批准号：
9232230
负责人：
Leslie C Griffith
金额：
$ 20.31万
依托单位：
BRANDEIS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-01 至 2018-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9232230
关键词：
Accounting Animal Model Antibodies Automobile Driving Behavior Behavioral Behavioral Genetics Brain Cells Chemicals Clustered Regularly Interspaced Short Palindromic Repeats Code Color Communication Complex Courtship Development Drosophila genus Ectopic Expression Failure Feeding behaviors Generations Genes Genetic Glutamates Imagery Individual Invertebrates Label Learning Libraries Logic Maps Mediating Memory Methods Modeling Modification Nature Neurons Neurotransmitters Output Pathologic Pattern Peptides Phenotype Population Potassium Channel Process Protein Fragment Proteins Reporter Reproduction Resolution Role Sea Serotonin Signal Transduction Site Sleep Spatial Distribution Synapses Synaptic Vesicles System Techniques Technology Tetanus Toxin Traction Vesicle Visualization software addiction base gamma-Aminobutyric Acid homologous recombination in vitro Assay information model intersectionality memory consolidation mutant neurotransmitter release postsynaptic promoter protein reconstitution public health relevance reconstitution relating to nervous system segregation tool transmission process visual processing

项目摘要

DESCRIPTION (provided by applicant): The ability to understand how brains generate behavior both in normal and pathological situations relies on our understanding of the neural computations carried out by behavioral circuits. The nature of chemical communication between neurons is determined by the specific neurotransmitters released onto postsynaptic targets and it was thought for many years that neurons released a single type of transmitter onto all their targets. In recent years, however, it has become clear that key neurons in vertebrate and invertebrate circuits involved in addiction, memory formation, feeding behavior and reproduction contain neurons that violate this rule, releasing multiple neurotransmitters. In this proposal we develop genetic tools to allow mapping of the distribution of specific neurotransmitter release sites in single neurons in multiple colors by modifying the endogenous genetic loci of vesicular transporters for neurotransmitter with fluorescent proteins. This allows both accurate and complete accounting of transmitter release sites since the marker's generation and turnover rely on processes that control the presence of the endogenous protein. Single cell resolution is obtained via an intersectional version of this strategy in which split fluorescent proteins become reconstituted only in specific cells. There is no current technique in any system which can provide this level of resolution. This technology will provide the ability to determine, in complex neurons releasing several chemical substances, the spatial distribution of each of the chemicals and its relationship to downstream targets of that neuron. It will also allow the mapping of temporal changes, either developmental or plasticity-induced, in neurotransmitter release. The technique is developed initially for use in Drosophila, a model organism which has been immensely important for our understanding of both the genetic and circuit basis of behavior, but as a general strategy can also be adapted for use in mammalian brain.