Activity-dependent regulation of CaMKII and synaptic plasticity
CaMKII 和突触可塑性的活动依赖性调节
基本信息
- 批准号:9803208
- 负责人:
- 金额:$ 40.63万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-08-15 至 2023-05-31
- 项目状态:已结题
- 来源:
- 关键词:3&apos Untranslated RegionsAcuteAddressAdultAffectAffinity ChromatographyAllelesAxotomyBehaviorBehavioralBinding ProteinsBioinformaticsBiologicalBiological AssayBrainBrain DiseasesCandidate Disease GeneCellsChronicClustered Regularly Interspaced Short Palindromic RepeatsCommunicationComplexCoupledDevelopmentDistalDrosophila genusElementsEventGenesGeneticGenetic RecombinationGenetic TranslationGenomicsImageryIndividualInvertebratesLeadLearningMapsMemoryMessenger RNAMetabolismMolecularMusMushroom BodiesMutateNeuromuscular JunctionNeuronsOptical MethodsPatternPhylogenetic AnalysisPreparationProcessProtein BiosynthesisProteinsProteomeRNARNA InterferenceRNA-Binding ProteinsRegulationRegulatory PathwayReporterResearchRestRibosomesRoleSideSpecific qualifier valueStructureSynapsesSynaptic plasticitySystemTestingTimeTrans-ActivatorsTransgenesTranslationsUrsidae FamilyVertebratesWorkcalmodulin-dependent protein kinase IIcell typeexperimental studyfast axonal transportflygenome editingimmunoreactivityin vivoin vivo evaluationinsightlong term memorymature animalmutantpostsynapticprogramsresponsesynaptic functiontool
项目摘要
Abstract
Memories of salient events in our lives are part of what makes us individuals. CaMKII has been shown to be
required in both vertebrates and invertebrates for short-term (STM) and long-term memory (LTM). CaMKII
protein is enriched at synapses, and is synthesized locally in response to activity patterns that lead to LTM
formation. Both of these features require specific sequences present in the distal untranslated part of the
CaMKII mRNA. In spite of extensive work on local translation of CaMKII, several fundamental questions
remain unanswered:
Is there a requirement for somatic factors in local translation? We will disrupt the connection between
the cell body and the synapse to test whether transport of somatic material to the synapse is required for
activity-stimulated local synthesis of CaMKII.
How is the information specifying local translation encoded in mRNA? Using transgenes encoding
fluorescent reporters and real-time assays of new protein synthesis, we will determine what sequences are
required for CaMKII synaptic localization and activity-dependent translation.
What are the cellular components that read this information? We will do a bioinformatically-driven
candidate gene screen in parallel with RNA affinity purification to identify proteins that regulate basal and
plasticity-stimulated CaMKII accumulation.
How does disruption of local translation of CaMKII affect LTM? We will use conditional genome editing
to remove mRNA sequences that specify local translation or to replace them with specific mutants. We will
determine which cells in the adult learning circuit use this information during LTM formation.
This project utilizes cutting-edge genetic, cell biological and optical methods to address the molecular basis
of a phylogenetically-conserved mechanism of plasticity in a way that will further our understanding of
complex behaviors.
摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Leslie C Griffith其他文献
Sleep: hitting the reset button
睡眠:按下重置按钮
- DOI:
10.1038/nn0208-123 - 发表时间:
2008-02-01 - 期刊:
- 影响因子:20.000
- 作者:
Leslie C Griffith;Michael Rosbash - 通讯作者:
Michael Rosbash
Leslie C Griffith的其他文献
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{{ truncateString('Leslie C Griffith', 18)}}的其他基金
Activity-Dependent Regulation of CaMKII and Synaptic Plasticity
CaMKII 和突触可塑性的活动依赖性调节
- 批准号:
10817516 - 财政年份:2023
- 资助金额:
$ 40.63万 - 项目类别:
Neurotransmitter plasticity and regulation of behavior
神经递质可塑性和行为调节
- 批准号:
10445855 - 财政年份:2022
- 资助金额:
$ 40.63万 - 项目类别:
Neurotransmitter Plasticity and Regulation of Behavior
神经递质可塑性和行为调节
- 批准号:
10565933 - 财政年份:2022
- 资助金额:
$ 40.63万 - 项目类别:
Activity-dependent regulation of CaMKII and synaptic plasticity
CaMKII 和突触可塑性的活动依赖性调节
- 批准号:
10412935 - 财政年份:2019
- 资助金额:
$ 40.63万 - 项目类别:
Activity-dependent regulation of CaMKII and synaptic plasticity
CaMKII 和突触可塑性的活动依赖性调节
- 批准号:
10155607 - 财政年份:2019
- 资助金额:
$ 40.63万 - 项目类别:
CRCNS: Neural signals that maintain/refresh LTP and memory
CRCNS:维持/刷新 LTP 和记忆的神经信号
- 批准号:
9242345 - 财政年份:2017
- 资助金额:
$ 40.63万 - 项目类别:
Genetic tools for visualization of co-transmission in identified neurons
用于可视化已识别神经元中的共传递的遗传工具
- 批准号:
9232230 - 财政年份:2016
- 资助金额:
$ 40.63万 - 项目类别:
Genetic tools for visualization of co-transmission in identified neurons
用于可视化已识别神经元中的共传递的遗传工具
- 批准号:
9090625 - 财政年份:2016
- 资助金额:
$ 40.63万 - 项目类别:
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