DNA Extraction Service

筛选小鼠DNA是评估在我们的菌落中出生的小鼠的关键的第一步。利用DNA所决定的遗传特性，继续进行育种选择，选择实验用动物，保证近交系小鼠遗传完整性的维持。使用蛋白水解酶消化尾部样品，并使用Autogen GenePrep DNA分离机器人分离DNA。在2017财年，已经处理了超过60,000个样品。这是一个高度自动化的过程，非常具有成本效益。消耗性成本/尾巴约为0.50美元。对弗雷德里克设施的监督包括与合同工作人员的谈话和适当时的访问。

The screening of mouse DNA is a critical first step in evaluating mice born in our colonies. The genetic characteristics determined by the DNA is used to continue selection for breeding, to choose animals to utilize in experiments, and to assure the maintenance of genetic integrity of inbred strains of mice. Tail samples are digested using a proteolytic enzyme and the DNA is isolated utilizing an Autogen GenePrep DNA isolation robot. During FY17 over 60,000 samples have been processed. This is a highly automated process and is very cost effective. Expendable costs/tail are approximately $0.50. Oversight of the Frederick facility consists of conversations with contract staff and visits when appropriate.