The role of protease activated receptors on platelets

蛋白酶激活受体对血小板的作用

• 批准号: 9241436
• 负责人: Marvin Thomas Nieman
• 金额: $ 31.7万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2021-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9241436
• 关键词: Address; Adverse effects; Affect; Agonist; Alleles; Allosteric Site; Amides; Amino Acids; Antiplatelet Drugs; Aspirin; Binding; Binding Sites; Blood Platelets; Blood Vessels; Blood coagulation; Cells; Deuterium; Development; Dominant-Negative Mutation; Effectiveness; F2R gene; FDA approved; Family; Frequencies; G-Protein-Coupled Receptors; Genetic Polymorphism; Genotype; Goals; Hemorrhage; Hemostatic function; Heritability; Histidine; Homo; Human; Hybrids; Hydrogen; Hydroxyl Radical; Individual; Intracranial Hemorrhages; Lead; Ligand Binding; Ligands; Mass Spectrum Analysis; Membrane; Molecular; Molecular Conformation; Molecular Structure; Monitor; Myocardial Infarction; Output; Pathologic; Pathway interactions; Patients; Pharmacogenomics; Pharmacology Study; Physiological; Platelet Activation; Platelet aggregation; Population; Proteinase-Activated Receptors; Proteins; Receptor Activation; Receptor Signaling; Recording of previous events; Reporting; Research; Resistance; Resolution; Risk; Role; Signal Pathway; Signal Transduction; Single Nucleotide Polymorphism; Stroke; Structure; Surface; Techniques; Testing; Therapeutic; Thrombin; Thrombin Receptor; Thrombus; Treatment Protocols; Variant; acute coronary syndrome; clopidogrel; experience; insight; inter-individual variation; interest; molecular rearrangement; new therapeutic target; prevent; public health relevance; receptor; receptor function; response; standard of care; structural biology; therapeutic target; therapy resistant

 DESCRIPTION (provided by applicant): Platelets are defined by their essential roles in hemostasis and the formation of pathological thrombi. Therefore, the major focus of platelet research is focused on identifying and developing safer and more effective anti- thrombotic therapies. A second goal is to have the ability to predict the effectiveness of specific therapies across populations. Pharmacogenomics may allow us to predict who will respond to or be resistant to therapies. In order for this to be successful, there needs to be a clear relationship between specific polymorphisms and physiological output. Finally, the alleles need to be present at high enough frequency to warrant testing prior to the start of therapy. In the current project w will examine common polymorphisms of a platelet receptor (PAR4) to explain the differences in reactivities and response to an antagonist at the molecular level. We will determine the structural rearrangement of PAR4 upon activation by thrombin using structural mass spectrometry (amide hydrogen deuterium exchange (HDX), histidine HDX, and hydroxyl radical foot-printing) with purified PAR4 and PAR4 on cells and platelets. These studies will determine how the tethered ligand influences the overall conformation of the receptor. Pharmacological studies in human platelet show that the PAR4 sequence variants have dramatically different responses. For example, PAR4-120T is hyper-reactive and PAR4-296V is resistant to signaling. We will determine how the PAR4 variants affect the ligand binding site and the transition to the active conformation at the molecular level. Further, PAR4-296V suppresses the activity of other alleles suggesting that it forms dominant negative homodimers. We will examine the physical interaction between PAR4 variants, PAR1 and P2Y12 to determine how these receptors influence signaling pathways. Finally, we will determine how the PAR4 sequence variants influence platelet reactivity in acute coronary syndrome patients on the current standard of care (aspirin and a P2Y12 antagonist) and if the altered platelet reactivity due to PAR4 variants is exacerbated with the PAR1 antagonist vorapaxar. By analyzing the structural rearrangements of PAR4 following activation by thrombin, these studies will provide the first detailed description of the tethered ligand activation mechanism for the PAR family and have the potential to uncover allosteric sites that can be exploited therapeutically. More specifically, understanding how naturally occurring sequence variants influence PAR4's response may allow us to predict the most appropriate antiplatelet therapy for patients depending on their genotype.

 描述(由申请人提供)：血小板是根据其在止血和病理性血栓形成中的基本作用来定义的。因此，血小板研究的主要重点是寻找和开发更安全、更有效的抗血栓治疗方法。第二个目标是有能力预测特定疗法在整个人群中的有效性。药物基因组学可能使我们能够预测谁会对治疗产生反应或产生抗药性。为了成功，特定的多态和生理输出之间需要有明确的关系。最后，等位基因需要以足够高的频率出现，以保证在治疗开始之前进行测试。在目前的项目中，w将研究血小板受体(PAR4)的常见多态性，以在分子水平上解释对拮抗剂的反应性和反应性的差异。我们将用纯化的PAR4和PAR4在细胞和血小板上用结构质谱仪(酰胺氢氚交换(HDX)、组氨酸HDX和羟基自由基足迹)来确定凝血酶激活时PAR4的结构重排。这些研究将确定连接配体如何影响受体的整体构象。对人类血小板的药理学研究表明，PAR4序列变异体具有显著不同的反应。例如，PAR4-120T是高反应性的，而PAR4-296V是抵抗信号的。我们将在分子水平上确定PAR4变体如何影响配体结合位置和向活性构象的转变。此外，PAR4-296V抑制了其他等位基因的活性，表明它形成了显性的负同二聚体。我们将研究PAR4变体PAR1和P2Y12之间的物理相互作用，以确定这些受体如何影响信号通路。最后，我们将确定在目前的治疗标准下(阿司匹林和一种P2Y12拮抗剂)，PAR4序列变异如何影响急性冠脉综合征患者的血小板反应性，以及PAR1拮抗剂Vorapaxar是否加剧了由PAR4变异引起的血小板反应性改变。通过分析凝血酶激活后PAR4的结构重排，这些研究将首次详细描述 PAR家族的拴系配体激活机制，并有可能发现可用于治疗的变构位点。更具体地说，了解自然发生的序列变异如何影响PAR4‘S反应，可能使我们能够根据患者的基因预测最合适的抗血小板治疗。