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DMPA use and vaginal bacterial diversity among African women

非洲女性的 DMPA 使用和阴道细菌多样性

基本信息

批准号：
9270203
负责人：
Alison Christina Roxby
金额：
$ 27.12万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-12-15 至 2018-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9270203
关键词：
AIDS prevention Affect Africa South of the Sahara African Anaerobic Bacteria Archives Bacteria Bacterial Vaginosis Biological Clinical Communities Contraceptive Agents Contraceptive Usage Control Groups Data Depo Provera Development Devices Ecology Estrogens Exhibits Female Fred Hutchinson Cancer Research Center Gardnerella vaginalis Genital system HIV HIV Infections HIV risk Health High-Throughput Nucleotide Sequencing Hormones Incidence Inflammation Injection of therapeutic agent Kenya Laboratories Lactobacillus Linear Regressions Link Medroxyprogesterone 17-Acetate Metadata Methods Molecular Outcome Pathway interactions Plasma Polymerase Chain Reaction Population Postpartum Period Prevention strategy Prospective cohort Prospective cohort study Public Health Research Risk Risk Factors Sampling Scientific Advances and Accomplishments Specimen Statistical Data Interpretation Swab Taxon Technology Testing Time Vagina Woman Work cohort contraceptive microbicide falls high risk hormonal contraception improved indexing microbial microbial community microbiota rRNA Genes therapy design vaginal microbiome

项目摘要

Project Summary Depot-medroxyprogesterone acetate (DMPA), a contraceptive, has been associated with increased female acquisition of HIV through unknown mechanisms. We hypothesize that DMPA use increases vaginal species diversity, changing the vaginal microbiota in ways that promote genital inflammation and female HIV acquisition. We will use previously-collected vaginal swab samples from a prospective cohort of 81 postpartum Kenyan women who initiated DMPA and a control cohort of 25 women using non-hormonal contraception. Collaborating with the Fredricks laboratory at Fred Hutchinson Cancer Research Center, where these laboratory methods were pioneered, we will determine longitudinal changes in the vaginal microbiome using broad-range PCR with high-throughput sequencing. We will use taxon-specific quantitative PCR to determine quantities of the 12 most common vaginal bacteria and track their change over time. We will then correlate vaginal microbiota changes with peak DMPA levels, and assess the impact of DMPA on specific populations of vaginal anaerobes linked to HIV acquisition. The work proposed here leverages existing specimens collected specifically to assess biological consequences of DMPA use, and will use extensive socio-demographic and clinical metadata to perform multivariate statistical analyses comparing dynamic changes in microbiota species, richness and diversity with appropriate adjustment for confounding factors. Should this research reveal significant DMPA-related microbiota changes, our results would be applicable to ongoing efforts to create safer multi-purpose HIV prevention technologies and efforts to improve vaginal health in sub-Saharan Africa.

项目总结