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Therapeutic Irradiation and Brain Functions

治疗辐射和脑功能

基本信息

批准号：
9242504
负责人：
Susanna Rosi
金额：
$ 38.23万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-12-09 至 2021-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9242504
关键词：
Affect Animals Astrocytes Behavioral Brain Brain Neoplasms CCL2 gene Cellular Structures Chronic Clinical Clinical Trials Cognition Cognitive deficits Cranial Irradiation Data Discrimination Dose Environment Evolution Flow Cytometry Functional disorder Funding Genes Genetic Genetic Models Genetic Transcription Genotype Goals Hippocampus (Brain)Immune Immunohistochemistry Impaired cognition Infiltration Inflammation Inflammatory Inflammatory Response Innate Immune System Ionizing radiation Kinetics Late Effects Learning Light Link Macrophage Colony-Stimulating Factor Receptor Measures Mediating Memory Memory impairment Methods Microglia Molecular Multivariate Analysis Mus Myeloid Cells Nerve Degeneration Nervous System Physiology Neuraxis Neurons Oxidative Stress Pathogenicity Peripheral Pharmacology Phase Phenotype Play Population Predisposition Process Quantitative Reverse Transcriptase PCR Radiation Radiation Injuries Recruitment Activity Reporter Research Research Infrastructure Role Signal Transduction Structure Synapses Synaptic Transmission Temporal Lobe Testing Therapeutic Time Work biological adaptation to stress chemokine receptor clinically relevant cognitive change cognitive function combinatorial information processing injured innovation irradiation macrophage monocyte mouse model neuroinflammation new therapeutic target novel permissiveness prevent radiation-induced cognitive dysfunction relating to nervous system response synaptic function treatment strategy

项目摘要

Project Summary Therapeutic irradiation is commonly used to treat both primary and metastatic brain tumors and can cause a number of late effects including progressive cognitive dysfunction. There is no treatment currently available that can even partially reverse cognitive changes observed after radiation injury. Specifically, irradiation of the temporal lobe can profoundly affect the cellular structures mediating learning and memory. Ionizing radiation has also been consistently shown to activate several neuroinflammatory signaling cascades that can impact multiple neural processes and synaptic transmission ultimately causing disruptions in hippocampal function. Notably, resident microglia and infiltrating monocytes, the key cellular player in neuroinflammatory processes, have distinct embryological origins and also fulfill different functions. The mechanism/s by which activation of the inflammatory response affect cognitive functions after brain irradiation and the specific role of different myeloid cells remain elusive. Thus, there is a clear need to understand the mechanisms of radiation injury and inflammation to develop strategies for preventing cognitive decline following cranial irradiation. Recent work from our group during the previous funding period has shed light in these questions and revealed specific problems in the cellular and molecular mechanisms underlying radiation-induced memory deficits. Specifically our data demonstrates a direct link between CCL2/CCR2 and cognition. These results provide a mechanistic link between peripheral innate immune system and cognition after brain irradiation. In the current proposal we will evaluate the central hypothesis that therapeutic doses of cranial irradiation induce infiltration of peripheral monocytes that modifies the resident inflammatory response and promotes synaptic dysfunction and long term cognitive deficits. Aim 1: Determine the kinetics and inflammatory phenotype of radiation-induced myeloid cell alterations after single and hypofractionated therapeutic doses of irradiation. Aim 2: Evaluate the role of peripheral monocyte recruitment into the brain as a mechanistic driver of radiation-induced altered synaptic and cognitive functions. Aim 3: Determine if temporary depletion of myeloid cells prevent the loss of synaptic function and cognition after single and hypofractionated doses of radiation. Very little is known in regard to the evolution of radiation induced pathophysiology in the context of peripherally derived macrophage accumulation or inflammation, and how this relates to altered synaptic and cognitive function. Our final therapeutic goal is to modify the cognitive changes observed after radiation injury.

项目总结