Genetic Analysis of Resistance to Viral Infection

抗病毒感染的遗传分析

基本信息

批准号：
9087128
负责人：
BRUCE A BEUTLER
金额：
$ 262.77万
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-08-15 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9087128
关键词：
Adaptor Protein Complex 3 Adaptor Signaling Protein Address Affect Age Alleles Antiviral Agents Antiviral Response Area Autophagocytosis Biochemical Bunyavirus Infections CRSP3 gene Categories Cell physiology Cells Cellular Stress Cessation of life Child Communication Communities Competence Complex Computer Simulation Cryopreservation Cytomegalovirus Cytomegalovirus Infections DNA Dendritic Cells Derivation procedure Drosophila genus Elements Embryo Endosomes Extended Family Fibroblasts Funding Gene Expression Gene Targeting Generations Genes Genetic Genetic Materials Genetic Techniques Genomic DNA Goals Grant Gray unit of radiation dose HIV Health Herpesviridae Infections Homologous Gene Host Defense Host resistance Human I-kappa B Proteins Image Immune Immune response Immune system Individual Infection Insecta Institutes Interferon Type I Interferons Investigation Knock-out Knockout Mice Learning Life Lipopolysaccharides Mammalian Cell Mammals Maps Massive Parallel Sequencing Measures Mediating Medical center Medicine Membrane Protein Traffic Memory Methods Microbe Modeling Molecular Movement Murid herpesvirus 1 Mus Mutagenesis Mutation Natural Immunity Natural Killer Cells Natural regeneration Nucleic Acids Organism Participant Pathway interactions Pharmaceutical Preparations Physiology Pluripotent Stem Cells Population Post-Transcriptional Regulation Post-Translational Protein Processing Predisposition Principal Investigator Process Productivity Proliferating Proteins RNA RNA Interference Reaction Reagent Recording of previous events Regulation Research Resistance Resistance to infection Resolution Rift Valley fever virus Risk Role Signal Pathway Signal Transduction Small RNA Smallpox Speed Sting Injury Structural Models Systemic Lupus Erythematosus TLR7 gene Talents Technology Therapeutic Time Toll-like receptors Transcriptional Regulation Transmembrane Transport Universities Ursidae Family Vaccines Viral Viral Genes Viral Pathogenesis Virus Virus Diseases Work Writing Zinc Fingers antiviral immunity base combat coping deep sequencing design expression cloning fly forward genetics gene induction gene interaction genetic analysis genome sequencing human disease human mortality induced pluripotent stem cell member molecular targeted therapies mutant mutation screening new technology pathogen pressure prevent programs receptor resistance gene resistance mechanism response reverse genetics screening sensor simulation small molecule sound stem cell technology transcription factor viral DNA viral RNA whole genome

项目摘要

DESCRIPTION (provided by applicant): The present application extends a successful multifaceted investigation of host resistance to viral infection. The strengths of our approach include: 1) an unbiased component based on mutagenesis combined with a hypothesis-driven component; 2) the study of distantly related organisms (mice and Drosophila) to appreciate which elements of defense are conserved; 3) the embrace of new and powerful methods to support our efforts. In our work to date, we have collectively identified new sensors (e.g., LGP2; DICER-2) necessary for activation of antiviral defenses, and delineated pathways of response to viruses, both at a biochemical level and in terms of communication between cells. We have identified previously unknown molecular participants in host defense. Among these are channel proteins (SLC15A4; KCNJ8/SUR2), transcription factors (IKB;AKIRIN2), proteins concerned with membrane trafficking or organellar mobility (AP3B1; STING; TR1M56; ATG9A; UNC93B), cell stress (SLFN2), post-translational modification (TRIM56; TR1M23), and endosome physiology (SLC15A4). Some of these proteins are members of extended families and may open the way to broad new models of host defense. Others highlight the importance of intermediary steps in host defense (e.g., the movement of molecules within cells) in a way that has not been considered before. Each participating group (Dallas, Osaka, and Strasbourg) has its special talents, and these have been combined to take us beyond genetics per se, incorporating new technologies that will accelerate the discovery of essential elements of the host defense apparatus. We recognize that it is not enough to possess a list of parts to understand how a machine operates. As new proteins are shown to be essential for particular aspects of host defense, we will establish how they interact with one another and/or other proteins to support resistance; how they catalyze particular reactions within cells, and how they drive or suppress the expression of genes in what we see as a highly dynamic process. We view the continuation of this POl as an opportunity to build upon an approach with established productivity: one that has generated new molecules, concepts, and reagents for use by the scientific community as a whole. The POl has been, and will continue to be, highly collaborative in the exchange of methods, genetic materials, and most importantly, ideas, ultimately derived from genetics.

描述（由申请人提供）：本应用程序扩展了对病毒感染的宿主抗性的成功多面研究。我们方法的优势包括：1）基于诱变与假设驱动的成分相结合的无偏分量； 2）对远距离相关的生物（小鼠和果蝇）的研究，以欣赏哪些防御要素是保存的； 3）拥抱新的有力的方法来支持我们的努力。在迄今为止的工作中，我们共同确定了激活抗病毒防御的新传感器（例如LGP2； DICER-2），并在生化水平和细胞之间的通信方面都确定了对病毒反应的途径。我们已经确定了以前未知的分子参与者在主持人防御中。其中包括通道蛋白（SLC15A4； KCNJ8/SUR2），转录因子（IKB； Akirin2），与膜运输或细胞器迁移有关的蛋白质（AP3B1; Sting; TR1M56; TR1M56; ATG9A; atg9a; act slfn2），细胞应力（SLFN2），eTS-36 extrantical trimifienation and trim56 and and trim56生理学（SLC15A4）。其中一些蛋白质是大家庭的成员，可能为广泛的新型宿主防御模式开辟道路。其他人则以前所未有的方式强调了中间步骤在宿主防御中的重要性（例如，细胞内分子的运动）。每个参与小组（达拉斯，大阪和斯特拉斯堡）都有其特殊才能，并且这些才能结合起来，使我们超越了遗传学本身，并结合了新技术，这些技术将加速宿主防御设备的基本要素。我们认识到，拥有一系列零件来了解机器的运行方式是不够的。由于新蛋白被证明对于宿主防御的特定方面至关重要，因此我们将确定它们如何相互作用和/或其他蛋白质以支持抗性。它们如何催化细胞内的特定反应，以及它们如何驱动或抑制我们认为是高度动态过程的基因的表达。我们认为这种pol的延续是建立具有既定生产力的方法的机会：一种生成了整个科学界使用的新分子，概念和试剂。 POL一直是并且将继续在方法，遗传材料以及最重要的是思想的交换方面一直是高度协作的，最终是源自遗传学的。

项目成果

期刊论文数量（67）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Sensing viral RNAs by Dicer/RIG-I like ATPases across species.

DOI：
10.1016/j.coi.2015.01.009
发表时间：
2015-02
期刊：
Current opinion in immunology
影响因子：
7
作者：
Paro S;Imler JL;Meignin C
通讯作者：
Meignin C

Special delivery: granulin brings CpG DNA to Toll-like receptor 9.

特别递送：颗粒蛋白将 CpG DNA 带到 Toll 样受体 9。

DOI：
10.1016/j.immuni.2011.04.001
发表时间：
2011
期刊：
Immunity
影响因子：
32.4
作者：
Moresco,EvaMarieY;Beutler,Bruce
通讯作者：
Beutler,Bruce

TANK is a negative regulator of Toll-like receptor signaling and is critical for the prevention of autoimmune nephritis.

DOI：
10.1038/ni.1771
发表时间：
2009-09
期刊：
Nature immunology
影响因子：
30.5
作者：
通讯作者：

Verloren negatively regulates the expression of IMD pathway dependent antimicrobial peptides in Drosophila.

DOI：
10.1038/s41598-021-94973-0
发表时间：
2021-07-30
期刊：
Scientific reports
影响因子：
4.6
作者：
Prakash P;Roychowdhury-Sinha A;Goto A
通讯作者：
Goto A

Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid-inducible gene-I and melanoma differentiation-associated gene 5.

DOI：
10.1084/jem.20080091
发表时间：
2008-07-07
期刊：
The Journal of experimental medicine
影响因子：
0
作者：
Kato H;Takeuchi O;Mikamo-Satoh E;Hirai R;Kawai T;Matsushita K;Hiiragi A;Dermody TS;Fujita T;Akira S
通讯作者：
Akira S