Temporal inactivation of epithelial Isl1 in adult mice leads to incisor enamel defects

项目摘要/摘要 釉质是牙齿的外层覆盖物，它的独特之处在于它是我们身体中最硬的组织，也是 几乎没有不能再生的人体组织。这种人牙釉质不能再生的原因是 当牙齿萌出进入口腔时，成釉细胞或釉质形成细胞及其前体细胞的丧失。 因此，如果我们能引导正常情况下不会产生牙釉质的成体干细胞制造牙釉质，那将是非常强大的。 并解开其中涉及的分子机制。我已经生成了一个老鼠模型，可以 诱导从非釉质形成的成釉细胞产生异位成釉细胞，成体干细胞具有时空- 一种称为Islet1(Isl1)的基因的特异性失活。ISL1靶基因的鉴定及遗传网络 将为研究有关牙釉质形成的许多问题提供新的途径 成釉作用。对釉质发生的全面的分子理解对于潜在的未来是重要的 牙科现有诊断、预防和治疗方法的创新和改进。

Project Summary/Abstract Enamel is the outer covering of teeth and is unique in that it is the hardest tissue in our body and one of the few human tissues that cannot regenerate. This inability for human enamel to regenerate is attributed to the loss of ameloblasts or enamel-forming cells and their precursor cells upon eruption of teeth into the oral cavity. Thus, it would be powerful if we could direct adult stem cells that normally do not generate enamel to make ameloblasts and unravel the molecular mechanisms involved. I have generated a mouse model that can be induced to produce ectopic ameloblasts from non-enamel forming, adult stem cells with spatial- and temporal- specific inactivation of a gene called Islet1 (Isl1). The identification of ISL1 target genes and genetic networks will reveal new pathways for investigation into numerous questions regarding enamel formation or amelogenesis. A comprehensive molecular understanding of amelogenesis is important for potential future innovations and improvements to current diagnostic, preventive, and therapeutic methods in dentistry.