Cell and Tissue Imaging Shared Resources Core

项目概要/摘要-细胞和组织成像共享资源 细胞和组织成像共享资源（CTISR）的主要目标是使最先进的技术 以及为SJCCC研究人员和受训人员提供的细胞和组织成像分析工具。CTISR 由两个部门组成：由维多利亚弗罗利希博士领导的光学显微镜和由沙龙领导的电子显微镜 弗雷泽Frohlich和Frase在科学成像领域拥有90多年的经验。CTISR是 还配备了8名经验丰富的成像科学家，他们协助采集，分析，定量， 图像数据的展示和出版。CTISR工作人员与SJCCC成员合作， 开始一个项目，为实验设计、适当的成像模式、样本 准备和数据分析选项。工作人员直接参与标本制备、成像， 分析是确定的。CTISR还在适用时提供用户培训。在本报告所述期间 （2017财年），CTISR-LM与40个SJCCC实验室进行了60次咨询，并培训了95名新仪器用户。 在本报告所述期间取得的一些成就包括与Tanja Mittag博士（CBP）的合作， Richard Kriwacki（CBP）和J. Paul Taylor（NBTP）可视化和表征液-液相的作用 无膜细胞器形成和维持的过渡。这项工作目前正在扩大， 一个SJCCC资助的中心间协作，如主任的战略计划部分所述， 概况.此外，CTISR工作人员协助Charles Mullighan博士（HMP联合负责人）通过共聚焦成像记录 和原位多光子成像，由突变的IKZF 1，a 高危急性淋巴细胞白血病（ALL）的标志，并检测通过类维生素A治疗的逆转。 因此，目前正在对类维生素A治疗IKZF 1改变的费城染色体阳性ALL进行临床评价。 追求（Churchman等人，Cancer Cell，2015）。在本项目期间，CTISR为159个 由SJCCC成员撰写的出版物。其中，40个（25%）是跨方案的，34个（21%）是 方案内。CTISR工作人员是24篇（15%）出版物的共同作者，这表明他们的价值 专业知识介绍研究。CTISR资源为高影响力期刊上的70篇出版物做出了贡献 （影响因子>10），如Cell、Nature和Nature Genetics。在未来五年，CTISR计划 不断扩展和发展服务--包括教育和培训计划--以及技术， 作为Lattice Lightsheet和虚拟现实可视化，使SJCCC成员能够有效地追求新兴的 癌症研究方向CTISR工作人员将继续教育SJCCC成员可用的方法， 使用相关显微镜（CLEM）方法来加强癌症研究。通过利用和整合 高性能计算资源，CTISR将增强统计和分析工具，并协助 SJCCC成员将这些工具应用于他们的研究。

PROJECT SUMMARY/ABSTRACT—Cell and Tissue Imaging Shared Resource The primary goal of the Cell and Tissue Imaging Shared Resource (CTISR) is to make state-of-the-art technology and analysis tools for imaging cells and tissues available to SJCCC investigators and trainees. The CTISR consists of 2 divisions: Light Microscopy, led by Dr. Victoria Frohlich, and Electron Microscopy, led by Sharon Frase. Together, Frohlich and Frase have more than 90 years of experience in scientific imaging. The CTISR is further staffed by 8 experienced imaging scientists who assist with acquisition, analysis, quantification, presentation, and publication of imaging data. The CTISR staff collaborates with SJCCC members prior to beginning a project to provide guidance on experimental design, appropriate imaging modalities, sample preparation, and data-analysis options. The degree of direct staff involvement in specimen preparation, imaging, and analysis is determined. The CTISR also provides user training when applicable. During the reporting period (FY2017), CTISR-LM conducted 60 consultations with 40 SJCCC labs and trained 95 new instrument users. Selected accomplishments in the current reporting period include a collaboration with Drs. Tanja Mittag (CBP), Richard Kriwacki (CBP), and J. Paul Taylor (NBTP) to visualize and characterize the role of liquid–liquid phase transition in the formation and maintenance of membraneless organelles. This work is now being expanded in an SJCCC-funded inter-center Collaborative as described in the Strategic Plan section of the Director's Overview. Additionally, CTISR staff assisted Dr. Charles Mullighan (HMP Co-Leader) to document, by confocal and in situ multiphoton imaging, the perturbations and stem cell-like phenotype caused by mutated IKZF1, a hallmark of high-risk acute lymphoblastic leukemia (ALL) and to detect the reversal by treatment with retinoids. As a result, clinical evaluation of retinoids for IKZF1-altered Philadelphia chromosome-positive ALL is being pursued (Churchman et al., Cancer Cell, 2015). During the current project period, CTISR contributed to 159 publications authored by SJCCC members. Of these, 40 (25%) were interprogrammatic and 34 (21%) were intraprogrammatic. CTISR staff were co-authors of 24 (15%) of the publications, signifying the value of their expertise to the research presented. CTISR resources contributed to 70 publications in high-impact journals (impact factor >10) such as Cell, Nature, and Nature Genetics. Over the next five years, the CTISR plans to continuously expand and evolve services—including education and training programs—and technologies such as Lattice Lightsheet and virtual reality visualization, enabling SJCCC members to effectively pursue emerging directions in cancer research. CTISR staff will continue to educate SJCCC members on available methods to use correlative microscopy (CLEM) approaches to enhance cancer research. By leveraging and incorporating high-performance computing resources, the CTISR will augment statistical and analytical tools and assist SJCCC members in applying these tools to their research.