FV-1000 Scanner for the Core Optical Imaging Facility

用于核心光学成像设施的 FV-1000 扫描仪

• 批准号: 7388502
• 负责人: VICTORIA CENTONZE FROHLICH
• 金额: $ 34.88万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7388502
• 关键词: 6 year old; 7 year old; Aging; Biocompatible Materials; Biomedical Research; Cells; Communities; Computer software; Consultations; Corrosion; Coupling; Dental Schools; Diabetes Mellitus; Disease; Education; Electronics; Evaluation; Failure; Floods; Funding; Future; Hand; Health Sciences; Humidity; Imaging Techniques; Imaging technology; Individual; Laboratory Research; Life; Malignant Neoplasms; Mission; Optics; Oxidative Stress; Preparation; Program Research Project Grants; Research Infrastructure; Research Personnel; Research Project Grants; San Antonio Cancer Institute; Schools; Services; Shock; Specimen; Support of Research; System; Techniques; Technology; Texas; Time; Universities; Week; age related; animal tissue; day; endometriosis; experience; instrument; medical schools; optical imaging; repaired; theories

DESCRIPTION (provided by applicant): This request is for funds to purchase a multiphoton-ready Olympus Fluoview 1000 confocal scanner, coupling optics and software. This instrument will be a replacement for an FV500 system in the Core Optical Imaging Facility at the University of Texas Health Science Center at San Antonio. The Core Optical Imaging Facility fills a critical need of UTHSCSA investigators and neighboring scientific community by offering, access to technology for imaging fixed and living cells, tissues, and animals, consultation, education and assistance regarding the theory and application of optical imaging techniques, technical advice on specimen preparation techniques and probe selection. Since the Core Optical Imaging Facility was established in 1999 it has become an integral part of the biomedical research community currently servicing more than 70 research laboratories from the Graduate School, Medical School, and Dental School. The facility is designated as a core for the UTHSCSA, the San Antonio Cancer Institute, the Nathan Shock Center, and an NIA funded program project grant. Confocal and multiphoton are the most frequently used services offered by the facility. The current confocal systems in the facility are a 7 year old Olympus Fluoview 500 and a 6 year old Zeiss 510 NLO. On average these instruments are used 50 per week. In May 2006, the Core Optical Imaging Facility experienced a significant flood which damaged or compromised the entire FV500 confocal system. At the time of damage evaluation it was determined that only items showing obvious damage or corrosion would be approved for repair or replacement. Even though filters and electronics of the FV500 experienced 100% humidity for days after the flood occurred and extremely high humidity during the 2 months it took to dry out the walls, the FV500 scanhead was not approved for replacement. It is likely that components in the FV500 scanner are in danger of developing filter corrosion and de-lamination as well as corrosion in the gears and electronics. In anticipation of a future failure of the system we are requesting a replacement/upgrade of the scanner and software. Many investigators rely upon the availability of the confocal and current research projects would be significantly compromised should the system become unusable. Replacement with a new scanner will revitalize the system and make available technology that does not exist in the facility (e.g. spectral confocal). Relevance: Acquisition of the FV1000 scanner will enhance the infrastructure of the Core Optical Imaging Facility at UTHSCSA. This core supports the research mission of investigators with scientific emphasis in oxidative stress/damage in aging and aging related diseases, diabetes, and cell invasion studies in cancer and endometriosis, and biomaterials. The core also supports the education mission of the university through courses and individual hands-on experience of trainees

描述（由申请人提供）：本申请旨在获得资金，用于购买多光子就绪的Olympus Fluoview 1000共聚焦扫描仪、耦合光学器件和软件。该仪器将取代圣安东尼奥德克萨斯大学健康科学中心核心光学成像设施中的FV 500系统。核心光学成像设施满足了UTHSCSA调查人员和邻近科学界的迫切需要，提供固定和活细胞，组织和动物成像技术，咨询，教育和光学成像技术的理论和应用方面的援助，标本制备技术和探针选择的技术建议。自1999年成立以来，核心光学成像设施已成为生物医学研究界不可或缺的一部分，目前为研究生院，医学院和牙科学院的70多个研究实验室提供服务。该设施被指定为UTHSCSA，圣安东尼奥癌症研究所，内森休克中心和NIA资助的计划项目赠款的核心。共焦和多光子是该设施提供的最常用的服务。该机构目前使用的共焦系统是7年的Olympus Fluoview 500和6年的Zeiss 510 NLO。平均每周使用这些仪器50次。2006年5月，核心光学成像设施经历了一场严重的洪水，损坏或损害了整个FV 500共焦系统。在进行损坏评估时，确定只有显示出明显损坏或腐蚀的物品才可批准维修或更换。尽管FV 500的过滤器和电子设备在洪水发生后的几天内经历了100%的湿度，并且在墙壁干燥的2个月内经历了极高的湿度，但FV 500扫描头没有被批准更换。FV 500扫描仪中的组件可能会出现过滤器腐蚀和分层以及齿轮和电子设备腐蚀的危险。由于预计系统将来会出现故障，我们要求更换/升级扫描仪和软件。许多研究者依赖于共焦系统的可用性，如果系统无法使用，当前的研究项目将受到严重影响。更换新的扫描仪将使系统恢复活力，并提供设施中不存在的技术（例如光谱共焦）。相关性：购买FV 1000扫描仪将增强UTHSCSA核心光学成像设施的基础设施。该核心支持研究人员的研究使命，科学重点是衰老和衰老相关疾病的氧化应激/损伤，糖尿病，癌症和子宫内膜异位症的细胞侵袭研究，以及生物材料。该核心还通过课程和学员的个人实践经验支持大学的教育使命