FV-1000 Scanner for the Core Optical Imaging Facility

描述(由申请人提供)：此申请是为了获得资金，以购买一台支持多光子的奥林巴斯FluoView 1000共焦扫描仪、耦合光学元件和软件。该仪器将取代位于圣安东尼奥的德克萨斯大学健康科学中心核心光学成像设备中的FV500系统。核心光学成像设备满足了UTHSCSA研究人员和邻近科学界的迫切需求，为固定和活细胞、组织和动物提供成像技术，就光学成像技术的理论和应用提供咨询、教育和援助，提供关于样品制备技术和探针选择的技术建议。自从1999年建立核心光学成像设施以来，它已经成为生物医学研究社区的一个组成部分，目前为研究生院、医学院和牙学院的70多个研究实验室提供服务。该设施被指定为UTHSCSA、圣安东尼奥癌症研究所、内森休克中心和NIA资助的项目项目赠款的核心。共焦和多光子是该设施提供的最常用的服务。该工厂目前的共焦系统是一台使用了7年的奥林巴斯FluoView 500和一台使用了6年的蔡司510NLO。这些仪器平均每周使用50次。2006年5月，核心光学成像设施经历了一场严重的洪水，损坏或破坏了整个FV500共焦系统。在评估损坏时，确定只有明显损坏或腐蚀的物品才会获准修理或更换。尽管FV500的过滤器和电子设备在洪水发生后的几天里经历了100%的湿度，在干燥墙壁的两个月里经历了极高的湿度，但FV500扫描头没有被批准更换。FV500扫描仪中的组件可能存在滤镜腐蚀和分层以及齿轮和电子设备中的腐蚀的危险。鉴于系统未来可能出现故障，我们要求更换/升级扫描仪和软件。许多研究人员依赖共焦系统的可用性，如果该系统变得无法使用，当前的研究项目将受到严重影响。用新的扫描仪替换将使系统恢复活力，并提供设施中不存在的技术(例如光谱共焦)。相关性：收购FV1000扫描仪将增强UTHSCSA核心光学成像设施的基础设施。这一核心支持研究人员的研究任务，科学重点是衰老和衰老相关疾病、糖尿病、癌症和子宫内膜异位症的细胞侵袭研究以及生物材料中的氧化应激/损伤。该核心还通过课程和学员的个人实践经验来支持大学的教育使命

DESCRIPTION (provided by applicant): This request is for funds to purchase a multiphoton-ready Olympus Fluoview 1000 confocal scanner, coupling optics and software. This instrument will be a replacement for an FV500 system in the Core Optical Imaging Facility at the University of Texas Health Science Center at San Antonio. The Core Optical Imaging Facility fills a critical need of UTHSCSA investigators and neighboring scientific community by offering, access to technology for imaging fixed and living cells, tissues, and animals, consultation, education and assistance regarding the theory and application of optical imaging techniques, technical advice on specimen preparation techniques and probe selection. Since the Core Optical Imaging Facility was established in 1999 it has become an integral part of the biomedical research community currently servicing more than 70 research laboratories from the Graduate School, Medical School, and Dental School. The facility is designated as a core for the UTHSCSA, the San Antonio Cancer Institute, the Nathan Shock Center, and an NIA funded program project grant. Confocal and multiphoton are the most frequently used services offered by the facility. The current confocal systems in the facility are a 7 year old Olympus Fluoview 500 and a 6 year old Zeiss 510 NLO. On average these instruments are used 50 per week. In May 2006, the Core Optical Imaging Facility experienced a significant flood which damaged or compromised the entire FV500 confocal system. At the time of damage evaluation it was determined that only items showing obvious damage or corrosion would be approved for repair or replacement. Even though filters and electronics of the FV500 experienced 100% humidity for days after the flood occurred and extremely high humidity during the 2 months it took to dry out the walls, the FV500 scanhead was not approved for replacement. It is likely that components in the FV500 scanner are in danger of developing filter corrosion and de-lamination as well as corrosion in the gears and electronics. In anticipation of a future failure of the system we are requesting a replacement/upgrade of the scanner and software. Many investigators rely upon the availability of the confocal and current research projects would be significantly compromised should the system become unusable. Replacement with a new scanner will revitalize the system and make available technology that does not exist in the facility (e.g. spectral confocal). Relevance: Acquisition of the FV1000 scanner will enhance the infrastructure of the Core Optical Imaging Facility at UTHSCSA. This core supports the research mission of investigators with scientific emphasis in oxidative stress/damage in aging and aging related diseases, diabetes, and cell invasion studies in cancer and endometriosis, and biomaterials. The core also supports the education mission of the university through courses and individual hands-on experience of trainees