Dissecting the circuits that support high acuity spatial vision

项目总结 弱视是一种由早期关键期视力障碍引起的视力障碍。神经学 弱视的基础主要是用眼优势可塑性来研究的。我最近发现了一个 视皮层高空间敏锐度的明显临界期，其先于眼优势临界期。 在这个提案中，我的目标是利用这一关键的洞察力，剖析出负责的特定神经回路 对于高敏锐度的反应。使用大量皮质神经元的活体钙成像，我将确定 小鼠视皮层高空间频率调谐神经元的发育。我猜想 高锐度视觉的出现涉及到高空间频率的亚群的出现 神经元。在此之前，我发现抑制性神经元间移植可诱导高敏感度视力的恢复 弱视小鼠。我将使用钙成像来确定我们之前报道的移植是否会导致 弱视小鼠视力的恢复涉及到高空间频率调谐神经元的恢复。如果 这项工作的成功将提供对高视力视觉背后的神经回路的洞察，并可能导致 弱视研究的新视角！

PROJECT SUMMARY Amblyopia is a disorder of visual acuity caused by disruptions in vision during an early critical period. The neural basis for amblyopia has predominantly been studied using ocular dominance plasticity. I recently discovered a distinct critical period for high spatial acuity in visual cortex that precedes the ocular dominance critical period. In this proposal, I aim to take advantage of this key insight and dissect out the specific neural circuits responsible for high acuity responses. Using in vivo calcium imaging of large populations of cortical neurons, I will determine the development of high spatial frequency tuned neurons in mouse visual cortex. I hypothesize that the emergence of high acuity vision involves the emergence of a subpopulation of high spatial frequency tuned neurons. Previously, I showed inhibitory interneuron transplantation induces recovery of high acuity vision in amblyopic mice. I will use a calcium imaging to determine whether our previously reported transplant induced restoration of acuity in amblyopic mice involves the restoration of high spatial frequency tuned neurons. If successful, this work will be provide insight into the neural circuits underlying high acuity vision and may lead to new insight for amblyopia research.!