Dissecting the circuits that support high acuity spatial vision

项目摘要 弱视是一种在早期关键时期由于视力中断而引起的视力障碍。神经 弱视的基础主要是利用眼优势可塑性来研究的。我最近发现了一个 在视觉优势关键期之前的视觉皮层中的高空间敏锐度的独特关键期。 在这个建议中，我的目标是利用这一关键的见解，并剖析出具体的神经回路负责 对于高灵敏度反应。使用大量皮质神经元的体内钙成像，我将确定 小鼠视皮层高空间频率调谐神经元的发育。我假设 高敏锐度视力的出现涉及高空间频率调谐的亚群的出现， 神经元以前，我显示抑制性中间神经元移植诱导高敏度视力的恢复， 弱视小鼠。我将使用钙成像来确定我们先前报道的移植是否诱导了 弱视小鼠敏锐度的恢复涉及高空间频率调谐神经元的恢复。如果 成功的话，这项工作将为深入了解高敏锐度视觉的神经回路提供帮助，并可能导致 弱视研究的新见解。！

PROJECT SUMMARY Amblyopia is a disorder of visual acuity caused by disruptions in vision during an early critical period. The neural basis for amblyopia has predominantly been studied using ocular dominance plasticity. I recently discovered a distinct critical period for high spatial acuity in visual cortex that precedes the ocular dominance critical period. In this proposal, I aim to take advantage of this key insight and dissect out the specific neural circuits responsible for high acuity responses. Using in vivo calcium imaging of large populations of cortical neurons, I will determine the development of high spatial frequency tuned neurons in mouse visual cortex. I hypothesize that the emergence of high acuity vision involves the emergence of a subpopulation of high spatial frequency tuned neurons. Previously, I showed inhibitory interneuron transplantation induces recovery of high acuity vision in amblyopic mice. I will use a calcium imaging to determine whether our previously reported transplant induced restoration of acuity in amblyopic mice involves the restoration of high spatial frequency tuned neurons. If successful, this work will be provide insight into the neural circuits underlying high acuity vision and may lead to new insight for amblyopia research.!