Ionotropic Receptor Function in Anopheles gambiae

冈比亚按蚊的离子型受体功能

• 批准号: 9754563
• 负责人: Chloe Greppi
• 金额: $ 3.09万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9754563
• 关键词: Address; Affect; Afferent Neurons; Air; Alleles; Animals; Anopheles Genus; Anopheles gambiae; Antibodies; Antibody Specificity; Arthropod Vectors; Base Pairing; Behavior; Behavioral; Behavioral Assay; Bite; Blood; Body Temperature; CRISPR/Cas technology; Carbon Dioxide; Cessation of life; Cues; Culicidae; Dengue; Desiccation; Detection; Disease; Disease Vectors; Drosophila genus; Drosophila melanogaster; Electrophysiology (science); Esthesia; Family; Frameshift Mutation; Goals; Health; Human; Humidifier; Humidity; Immunohistochemistry; Infection; Insect Vectors; Insecta; Larva; Location; Malaria; Mediating; Molecular; Neurons; Odors; Oviposition; Pattern; Play; Public Health; Reproduction; Role; Sensory; Site; Stimulus; Stream; Temperature; Temperature Sense; Testing; Vector-transmitted infectious disease; Work; Zika Virus; behavior test; behavioral response; behavioral study; chikungunya; experimental study; feeding; fight against; fly; genome editing; human disease; insight; interest; loss of function mutation; member; mutant; receptor; receptor function; response; vector; vector mosquito

Project Summary/ Abstract Mosquitoes are important vectors for widespread human diseases from malaria to dengue, zika virus and chikungunya, and temperature and moisture detection are critical for supporting mosquito survival, reproduction and host-seeking. Accurately sensing environmental temperature is important for maintaining optimal body temperature, while sensing humidity is important for avoiding desiccation. Temperature and humidity are also important cues for host-seeking and blood-feeding, and moisture is important for oviposition as mosquito larvae are aquatic. But despite their importance, the molecular mechanisms underlying thermo- and hygro-sensing in mosquitoes remain largely unknown. Recent work in the Garrity lab has identified key members of a receptor family that modulate cold sensing and moisture sensing in the fruit fly Drosophila melanogaster. These receptors are conserved in vector mosquitoes and highly expressed in their antennae, consistent with the notion that they serve similar sensory roles. Anopheles gambiae is the major vector for malaria and was responsible for over two hundred million infections in 2015. Understanding the molecular mechanisms underlying temperature and humidity sensing in Anopheles gambiae could provide key insights for strategies to control this important disease vector and has potential implications for similar mechanisms in other vector mosquitoes. In my preliminary studies, I have used CRISPR/Cas9 genome editing in Anopheles gambiae to create mutants containing putative loss-of-function mutations in these receptors. I propose to use a combination of electrophysiology, immunohistochemistry and behavioral studies to establish the role(s) of these receptors in thermo- and hygro-sensing in the mosquito Anopheles gambiae. My work will address the sensory function of these receptors in mosquitoes and probe their potential roles in host-seeking behavior.

项目摘要/摘要 蚊子是从疟疾到登革热，寨卡病毒和 基孔肯亚（Chikungunya）以及温度和水分检测对于支持蚊子生存至关重要， 繁殖和寻求主机。准确感测环境温度对于维护很重要 最佳体温，同时感应湿度对于避免干燥很重要。温度和 湿度对于寻求宿主和喂食的湿度也很重要，水分对于产卵很重要 因为蚊子幼虫是水生的。但是尽管它们的重要性，但热热的分子机制 蚊子中的透明度仍然在很大程度上未知。 Garrity Lab的最新工作已经确定了调节冷感应的受体家族的关键成员 和果蝇果蝇中的水分传感。这些受体在载体中是保守的 蚊子和高度表达在其天线中，与它们具有相似感觉的概念一致 角色。 Anopheles Gambiae是疟疾的主要向量，负责超过200万 2015年感染。了解温度和湿度感测的分子机制 Anopheles Gambiae可以为控制这一重要疾病向量的策略提供关键见解，并具有 对其他载体蚊子中类似机制的潜在影响。 在我的初步研究中，我在Anopheles Gambiae使用CRISPR/CAS9基因组编辑来创建 这些受体中包含假定功能丧失突变的突变体。我建议将 电生理学，免疫组织化学和行为研究，以确定这些受体在 蚊子冈比亚蚊子中的热和透明敏感性。我的工作将解决 这些受体在蚊子中，并探究它们在寻求宿主的行为中的潜在作用。