Single-Particle Characterization of Nanoparticle Bioconjugates

纳米颗粒生物共轭物的单颗粒表征

• 批准号: 9888384
• 负责人: Kathryn Mayer
• 金额: $ 14.7万
• 依托单位: UNIVERSITY OF TEXAS SAN ANTONIO
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9888384
• 关键词: Adherence; Antibodies; Astronomy; Biomedical Technology; Contrast Media; Country; Data; Development; Drug Delivery Systems; Engineering; Enzymes; Epidermal Growth Factor Receptor; Exposure to; Fluorescence Microscopy; Goals; Gold; Health Sciences; Heterogeneity; Hispanic-serving Institution; IgG1; Image; Individual; Institution; Label; Location; Measures; Methods; Mus; Optics; Physics; Play; Polymers; Polystyrenes; Population; Preparation; Procedures; Process; Quality Control; Rattus; Research; Resolution; Role; Sampling; Source; Spatial Distribution; Surface; System; Techniques; Texas; Training; Universities; Variant; Work; antibody conjugate; base; cancer biomarkers; contrast imaging; density; design; doctoral student; improved; nano; nanoparticle; particle; photothermal therapy; physical science; preservation; single molecule

Nanoparticles play several roles in biomedical technology, including as imaging contrast agents, heat sources for photothermal therapy, and as drug delivery vehicles. In many of these applications, nanoparticles are functionalized with specific antibodies for targeting purposes. Ideally, antibodies would be distributed equally among the nanoparticles in a preparation and evenly distributed on each nanoparticle’s surface; however, this is an overly simplistic picture. In reality, there are likely to be variations in the number of antibodies per nanoparticle, their spatial distribution on the surface, and the degree to which their function is preserved. In order to characterize nanoparticle bioconjugates, and to design improved ones, the details of the surface functionalization must be better understood. The following two aims will pave the way towards this goal. (1) The distributions of the number of antibodies per nanoparticle within a nanoparticle preparation will be measured at the single-particle level using microwell array analysis, for both gold and polystyrene nanoparticles. (2) The spatial arrangement of antibodies on the surfaces of individual nanoparticles will be imaged using sub-diffraction-limited fluorescence microscopy. The preparation methods will be optimized to produce nanoparticles with uniform functionalization in terms of the number and spatial distribution of molecules on the surface. Characterizing and optimizing the surface functionalization is important for the development of nanoparticle-based therapies. Improving the uniformity and quality of antibody functionalization will lead to improved targeting. The project will be carried out in the Department of Physics and Astronomy at the University of Texas at San Antonio, which is one of the country’s largest designated Hispanic Serving Institutions. An important part of this SCORE project will be the training of Ph.D. students from groups historically underrepresented in the physical and health sciences, and the elevation of research at this growing institution.

纳米粒子在生物医学技术中发挥着多种作用，包括成像造影剂、热源

项目成果

Synthesis and bioconjugation of alkanethiol-stabilized gold bipyramid nanoparticles.

• DOI: 10.1088/1361-6528/abe823
• 发表时间: 2021-03-09
• 期刊: Nanotechnology
• 影响因子: 3.5
• 作者: Abdul-Moqueet MM;Tovias L;Lopez P;Mayer KM
• 通讯作者: Mayer KM

Multiphysics Modeling of Plasmonic Photothermal Heating Effects in Gold Nanoparticles and Nanoparticle Arrays.

• DOI: 10.1021/acs.jpcc.0c02443
• 发表时间: 2020-08-06
• 期刊: The journal of physical chemistry. C, Nanomaterials and interfaces
• 作者: Manrique-Bedoya S;Abdul-Moqueet M;Lopez P;Gray T;Disiena M;Locker A;Kwee S;Tang L;Hood RL;Feng Y;Large N;Mayer KM
• 通讯作者: Mayer KM