Slide Scanner for the ONPRC Imaging and Morphology Core

项目总结 俄勒冈州国家灵长类研究中心的成像和形态支持核心(IMSC) (ONPRC)提议获得资金，用于一种全自动、高容量、高分辨率的幻灯片扫描仪，该扫描仪可以 可同时适应明场成像和荧光成像。IMSC提供全面、专业和 为调查人员提供经济高效的高级光学显微镜支持，使他们能够访问最先进的 增强协作、多学科研究的显微技术。幻灯片扫描仪将是 位于显微镜核心设施中，在核心工作人员的专家支持下实现最大限度的利用。 ONPRC调查人员在此应用程序中未使用的仪器时间将提供给其他科学家，网址为 俄勒冈健康与科学大学，从而扩大了整体利益。购买幻灯片扫描仪将 对ONPRC的生物医学研究产生很大影响，并将扩大NIH资助的项目的范围。 《研究项目》部分详述的所有项目都需要高容量和高性能的产品。 可重复性扫描用于大组织区域的分析，覆盖数百个视野，而不是 在标准显微镜上获得的单个视场，涉及到将单个图像后处理成 需要耗费大量时间和精力的大型蒙太奇。在仔细评估了商业上可用的 市场上的幻灯片扫描仪和用户组的输入，我们正在请求资金购买奥林巴斯 VS120-6S幻灯片扫描仪。

PROJECT SUMMARY The Imaging and Morphology Support Core (IMSC) at the Oregon National Primate Research Center (ONPRC) proposes to acquire funds for a fully automated, high-capacity, high-resolution slide scanner that can accommodate both bright-field and fluorescence imaging. The IMSC provides comprehensive, expert, and cost-effective advanced light microscopy support to investigators by providing them access to state-of-the-art microscopy technologies that enhance collaborative, multidisciplinary research. The slide scanner will be located in the microscopy core facility for maximum utilization with expert support from the core staff. Instrument time not used by the ONPRC investigators on this application will be available to other scientists at Oregon Health & Science University, thereby extending the overall benefits. Acquisition of a slide scanner will have a high impact on the biomedical research at ONPRC and will expand the scope of NIH-funded projects. All of the projects detailed in the “Research Projects” section are in need of high-capacity and high- reproducibility scanning for analyses in large tissue areas, covering hundreds of fields of view, rather than the single fields of view acquired on a standard microscope, and involves post-processing of individual images into large montages which require enormous time and effort. After careful evaluation of the commercially available slide scanners in the market and input from the user group, we are requesting funds to purchase an Olympus VS120-6S slide scanner.