Sequencing and Epigenetics Core

测序和表观遗传学核心(核心C) 核心摘要 CCHI计划中的拟议项目将使用遗传方法来定义全面的分子 不同疾病不同方面免疫反应中特异性免疫细胞的特性 从血液和人体临床组织(淋巴结和肺组织)接种疫苗。面对内在的挑战， 在处理人类样本、CCHI测序和表观遗传学时对细胞丰度和异质性的影响 与任何其他外部设施不同，CORE将提供一套独特而全面的程序，尤其是 经验证可分离和处理少量免疫细胞群，并支持下游单个 细胞和批量转录学以及表观遗传学研究。首先，测序核心的集中团队将 协助所有CCHI项目使用者认真采集少量免疫细胞，足够 为建议中所列的所有转录和表观遗传学分析处理和储存样品。第二, 测序核心将执行所有微型散装RNA-SEQ以及单细胞RNA测序，并将 为整个转录组分析、TCR和BCR高变量序列提取提供支持 批量和单细胞rna-seq数据集。最后，测序核心将进行微尺度文库准备 用于表观遗传学分析(ATAC-和CHIP-SEQ)。

Sequencing and Epigenetics Core (Core C) Core Summary The proposed projects in this CCHI program will use genetic approaches to define comprehensive molecular properties of specific immune cells in different aspects of immune responses in different diseases and after vaccination from blood and human clinical tissues (lymph nodes and lung tissue). Facing the inherent challenge, of cell abundance and heterogeneity when working with human samples, the CCHI Sequencing and Epigenetics Core, unlike any other external facility, will provide a unique and comprehensive set of procedures especially validated for isolating and processing small numbers of immune cell populations and support downstream single- cell and bulk transcriptomic as well as epigenetic studies. First, the centralized team of the Sequencing Core will provide assistance to all CCHI project users for careful collection of small number of immune cells, adequate processing and storage of samples for all transcriptomic and epigenetic assays listed in the proposal. Second, the Sequencing Core will perform all micro-scaled bulk RNA-seq as well as single-cell RNA sequencing and will provide support for the whole transcriptome analysis, TCR and BCR highly variable sequence extraction from bulk and single-cell RNA-seq datasets. Finally, the Sequencing Core will perform micro-scaled library preparation for epigenetic assays (ATAC- and ChIP-Seq).