Host-Microbial Analytic and Repository Core

宿主微生物分析和储存库核心

• 批准号: 9762893
• 负责人: GARY D. WU
• 金额: $ 18.17万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9762893
• 关键词: Address; Animal Model; Bioinformatics; Biological Models; Biometry; Clinical; Clinical Data; Collection; Computerized Medical Record; Consult; DNA Sequencing Facility; Data Set; Development; Digestive System Disorders; Disease; Environmental Risk Factor; Epigenetic Process; Evaluation; Funding; Gene Expression; Generations; Genes; Genomics; Human; Human Biology; Human Microbiome; Human Subject Research; Immune response; In Vitro; Intervention; Intervention Studies; Intestines; Investments; Joints; Lead; Liver diseases; Mammalian Cell; Messenger RNA; Metadata; Microbe; Microbial Taxonomy; Mind; Modality; Modification; Molecular; Molecular Biology; Mucosal Immunity; Pancreatic Diseases; Pathogenesis; Pediatric Hospitals; Pennsylvania; Phenotype; Philadelphia; Physiology; Play; Population; Production; Property; Proteins; Research; Role; Services; Shotguns; System; Testing; Time; Translating; Translations; Universities; base; design; experimental study; gut microbiome; gut microbiota; host-microbe interactions; human subject; instrument; interest; member; metabolome; metabolomics; metagenomic sequencing; microbial; microbial host; microbiome; microbiome analysis; microbiota; mouse model; next generation sequencing; potential biomarker; pre-clinical; pre-clinical research; precision medicine; prevent; programs; repository; response; success; transcriptomics

PROJECT SUMMARY (HOST-MICROBIAL ANALYTICS & REPOSITORY CORE) There is growing evidence for the importance of environmental factors, such as the microbiome, playing a critical role in the pathogenesis of many digestive, liver and pancreatic diseases. There is much interest in the characterization of the microbiome to identify potential biomarkers relevant for precision medicine as well as the modification of the human microbiome as a modality to prevent and/or treat diseases. To facilitate research focused on host-microbial interactions and their relevance to digestive, liver and pancreatic diseases, the Center for Molecular Studies in Digestive and Liver Diseases (CMSDLD) has developed a Host-Microbial Analytic and Repository Core (H-MARC) with the following two Specific Aims: 1) To provide critical analytic services the characterize analytes (i.e. genomics, transcriptomics, metabolomics) in both microbes and their mammalian hosts and 2) To provide expertise that will allow CMSDLD members to extend pre-clinical in vitro and animal model research into the human clinical domain. In Specific Aim 1, to support the analysis of the mammalian host, H-MARC will provide access to high-end instruments designed to quantify gene expression at the mRNA and protein levels as well as access to FACS for the characterization of mammalian cell populations. Genomic analysis will be provided via Penn's Next Gen Sequencing Core. To support the analysis of the microbiota, H-MARC will support experiments involving microbial cultures as well as the analysis of metabolites via targeted metabolomics. Computational and biostatistical support to analyze microbiome datasets associated with clinical metadata will also be provided through full time biostatisticians in H-MARC who will interface with the PennCHOP Microbiome Program. In Specific Aim 2, H-MARC will support human subject research by providing a robust Human Biospecimen Repository via a LabVantage-based LIMS system as well as the collection of robust annotated clinical data for IBD phenotyping via the EPIC EMR. Importantly, to facilitate human subject research translating preclinical research into the clinical domain, H-MARC will provide advice and expertise in the development of human intervention studies. Ultimately, H-MARC services are designed to facilitate integrated analyses of host-microbial interactions at the preclinical and clinical interface.

项目总结（宿主微生物分析和储存库核心） 越来越多的证据表明，环境因素的重要性，如微生物组，发挥作用， 在许多消化、肝脏和胰腺疾病的发病机制中起关键作用。有很多兴趣在 微生物组的表征，以确定与精准医学相关的潜在生物标志物，以及 将人类微生物组的修饰作为预防和/或治疗疾病的方式。以促进 研究重点是宿主-微生物相互作用及其与消化、肝脏和胰腺疾病的相关性， 消化和肝脏疾病分子研究中心（CMSDLD）开发了一种宿主微生物 分析和知识库核心（H-MARC）有以下两个具体目标：1）提供关键分析 服务于微生物及其代谢物中的特征分析物（即基因组学，转录组学，代谢组学）。 哺乳动物宿主和2）提供专业知识，使CMSDLD成员能够在体外扩展临床前 和动物模型研究进入人类临床领域。在具体目标1中，为了支持对 在哺乳动物宿主中，H-MARC将提供用于定量基因表达的高端仪器 在mRNA和蛋白质水平上，以及使用流式细胞术表征哺乳动物细胞 人口。基因组分析将通过Penn的Next Gen Sequencing Core提供。来支持分析 H-MARC将支持涉及微生物培养的实验以及对微生物的分析。 通过靶向代谢组学研究代谢物。为微生物组分析提供计算和生物统计支持 与临床元数据相关的数据集也将通过全职生物统计学家在H-MARC中提供 他将与PennCHOP微生物组计划进行对接。在Specific Aim 2中，H-MARC将支持人类 通过基于LabVantage的LIMS系统提供强大的人类生物标本库， 以及通过EPIC EMR收集IBD表型的稳健注释临床数据。重要的是， 为了促进人类受试者研究将临床前研究转化为临床领域，H-MARC将 为人类干预研究的发展提供建议和专业知识。H-MARC服务 旨在促进临床前和临床上宿主-微生物相互作用的综合分析 接口.