Core E: Immunology Core

核心 E：免疫学核心

• 批准号: 9762568
• 负责人: JEFFREY MILUSH
• 金额: $ 33.16万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9762568
• 关键词: AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Address; Aging; Applications Grants; Award; Basic Science; Biological Assay; Biological Markers; Bone Marrow; Businesses; Cardiovascular system; Cell Separation; Cells; Clinical Management; Clinical Treatment; Collaborations; Communities; Comorbidity; Consultations; Custom; Cytometry; Data Analyses; Detection; Development; Discipline; Education; Evaluation; Flow Cytometry; Funding; Genes; Goals; Gut associated lymphoid tissue; HIV; Immune; Immunoassay; Immunology; Immunology procedure; Immunophenotyping; In Situ; Interdisciplinary Study; International; Internships; Laboratories; Leadership; Leukapheresis; Lymphocyte Subset; Marketing; Medicine; Mentors; Mission; Modeling; Monitor; Morbidity - disease rate; Peer Review; Peripheral Blood Mononuclear Cell; Phenotype; Procedures; Production; Publications; Quality Control; RNA; Research; Research Personnel; Research Project Grants; Research Support; Resources; San Francisco; Services; Signal Pathway; Signal Transduction; Site; Sorting - Cell Movement; Technology; Thinness; Tissues; Training; Training and Education; Translational Research; Uganda; career; cytokine; cytotoxicity; immune function; improved; instrumentation; lectures; lymph nodes; meetings; new technology; outreach; tissue processing

The UCSF-Gladstone CFAR Immunology Core supports immunology research aimed at improving the clinical management and treatment of HIV/AIDS and related co-morbidities, both locally and globally, and informing the development of an effective HIV cure strategy. The Immunology Core enables highly collaborative multidisciplinary and translational research by providing access to immunology expertise, instrumentation and a wide range of immunology assay services, including multiparameter flow cytometric assays of immune cell phenotype and immune function (proliferation, cytotoxicity, cytokine production and cell signaling); specialized tissue processing of bone marrow, lymph node and gut- associated lymphoid tissues plus isolation of specific subsets of lymphocytes by immunomagnetic bead or FACS sorting and analysis of downstream signaling pathways within these purified cell subsets. The Core provides services from two different campus sites including the Core Immunology Laboratory located within the Division of Experimental Medicine at Zuckerberg San Francisco General and the Gladstone Flow Core located at Mission Bay. During the last funding period, the Immunology Core contributed to 115 peer-reviewed publications, provided mentoring and training to 45 fellows and junior investigators and 15 laboratory interns. The Immunology Core has provided flow cytometer access to 241 users. The Core responded to changing needs of CFAR investigators by introducing 21 new assays and services and actively supported CFAR scientific themes including studies of aging and co- morbidity; latency and eradication; and international studies. Over the next five years, the Immunology Core will continue to support these themes, and respond to emerging questions both locally and at resource-limited international sites. Overall, the Core provides four complementary lines of service including: 1) The provision of state-of-the-art immunology services to support HIV research and expand capacity; 2) The evaluation and optimization of new immunological assays, technologies and related services for CFAR investigators; 3) Building capacity at international sites; and 4) Training, mentoring and education of early-career investigators, senior investigators from other disciplines, and laboratory staff.

UCSF-Gladstone CFAR 免疫学核心支持旨在改善免疫学研究 本地和全球艾滋病毒/艾滋病及相关合并症的临床管理和治疗， 并为制定有效的艾滋病毒治疗策略提供信息。免疫学核心能够高度 通过提供免疫学专业知识进行协作多学科和转化研究， 仪器和广泛的免疫学检测服务，包括多参数流 免疫细胞表型和免疫功能（增殖、细胞毒性、细胞因子 生产和细胞信号传导）；骨髓、淋巴结和肠道的专门组织处理 相关淋巴组织加上通过免疫磁珠分离特定淋巴细胞亚群 或 FACS 对这些纯化细胞亚群内的下游信号通路进行分选和分析。这 Core 从两个不同的校园站点提供服务，包括核心免疫学实验室 位于扎克伯格旧金山综合医院实验医学部和 Gladstone Flow Core 位于 Mission Bay。在上一个资助期间，免疫学核心 为 115 份同行评审出版物做出了贡献，为 45 名研究员和初级人员提供了指导和培训 调查员和 15 名实验室实习生。免疫学核心提供了流式细胞仪访问 241 个用户。核心响应 CFAR 调查员不断变化的需求，推出了 21 个新的 化验和服务，并积极支持 CFAR 科学主题，包括衰老和协同研究 发病率；潜伏期和根除；和国际研究。未来五年，免疫学 Core 将继续支持这些主题，并回答本地和全球新出现的问题 资源有限的国际站点。总体而言，核心提供四种互补的服务线 包括： 1) 提供最先进的免疫学服务来支持艾滋病毒研究并扩大 容量; 2）新的免疫学检测、技术及相关的评估和优化 为 CFAR 调查员提供服务； 3）国际站点的能力建设；和 4) 早期职业研究者、其他高级研究者的培训、指导和教育 学科和实验室人员。