Core E: Immunology Core

核心 E：免疫学核心

• 批准号: 10249377
• 负责人: JEFFREY MILUSH
• 金额: $ 32.95万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10249377
• 关键词: AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Address; Aging; Applications Grants; Award; Basic Science; Biological Assay; Biological Markers; Bone Marrow; Businesses; Cardiovascular system; Cell Separation; Cells; Clinical Management; Clinical Treatment; Collaborations; Communities; Consultations; Custom; Cytometry; Data Analyses; Detection; Development; Discipline; Education; Evaluation; Flow Cytometry; Funding; Genes; Goals; Gut associated lymphoid tissue; HIV; Immune; Immunoassay; Immunology; Immunology procedure; Immunophenotyping; In Situ; Interdisciplinary Study; International; Internships; Laboratories; Leadership; Leukapheresis; Lymphocyte Subset; Marketing; Medicine; Mentors; Mission; Modeling; Monitor; Morbidity - disease rate; Peer Review; Peripheral Blood Mononuclear Cell; Phenotype; Procedures; Production; Publications; Quality Control; RNA; Research; Research Personnel; Research Project Grants; Research Support; Resources; San Francisco; Services; Signal Pathway; Signal Transduction; Site; Sorting - Cell Movement; Technology; Thinness; Tissues; Training; Training and Education; Translational Research; Uganda; career; comorbidity; cytokine; cytotoxicity; immune function; improved; instrumentation; latent HIV reservoir; lectures; lymph nodes; meetings; new technology; outreach; tissue processing

The UCSF-Gladstone CFAR Immunology Core supports immunology research aimed at improving the clinical management and treatment of HIV/AIDS and related co-morbidities, both locally and globally, and informing the development of an effective HIV cure strategy. The Immunology Core enables highly collaborative multidisciplinary and translational research by providing access to immunology expertise, instrumentation and a wide range of immunology assay services, including multiparameter flow cytometric assays of immune cell phenotype and immune function (proliferation, cytotoxicity, cytokine production and cell signaling); specialized tissue processing of bone marrow, lymph node and gut- associated lymphoid tissues plus isolation of specific subsets of lymphocytes by immunomagnetic bead or FACS sorting and analysis of downstream signaling pathways within these purified cell subsets. The Core provides services from two different campus sites including the Core Immunology Laboratory located within the Division of Experimental Medicine at Zuckerberg San Francisco General and the Gladstone Flow Core located at Mission Bay. During the last funding period, the Immunology Core contributed to 115 peer-reviewed publications, provided mentoring and training to 45 fellows and junior investigators and 15 laboratory interns. The Immunology Core has provided flow cytometer access to 241 users. The Core responded to changing needs of CFAR investigators by introducing 21 new assays and services and actively supported CFAR scientific themes including studies of aging and co- morbidity; latency and eradication; and international studies. Over the next five years, the Immunology Core will continue to support these themes, and respond to emerging questions both locally and at resource-limited international sites. Overall, the Core provides four complementary lines of service including: 1) The provision of state-of-the-art immunology services to support HIV research and expand capacity; 2) The evaluation and optimization of new immunological assays, technologies and related services for CFAR investigators; 3) Building capacity at international sites; and 4) Training, mentoring and education of early-career investigators, senior investigators from other disciplines, and laboratory staff.

UCSF-Gladstone CFAR免疫学核心支持免疫学研究，旨在改善免疫系统。 在本地和全球对艾滋病毒/艾滋病及相关并发症进行临床管理和治疗， 并为制定有效的艾滋病毒治疗战略提供信息。免疫学核心能够高度 通过提供免疫学专业知识，进行多学科和转化研究的合作， 仪器和广泛的免疫分析服务，包括多参数流 免疫细胞表型和免疫功能（增殖、细胞毒性、细胞因子 生产和细胞信号传导）;骨髓、淋巴结和肠的专门组织处理， 相关淋巴组织加免疫磁珠分离特定淋巴细胞亚群 或对这些纯化的细胞亚群内的下游信号传导途径进行FACS分选和分析。的 核心提供服务，从两个不同的校园网站，包括核心免疫学实验室 位于Zuckerberg San弗朗西斯科综合医院的实验医学部， 格莱斯顿流动核心位于使命湾。在上一个资助期间，免疫学核心 为115份经同行审查的出版物供稿，为45名研究员和初级研究员提供辅导和培训 调查员和15名实验室实习生免疫学核心提供了流式细胞仪访问， 241个用户。核心小组对CFAR调查人员不断变化的需求作出了回应， 分析和服务，并积极支持CFAR的科学主题，包括老化和协同研究， 发病率;潜伏期和根除;以及国际研究。在接下来的五年里，免疫学 核心将继续支持这些主题，并回应当地和国际社会新出现的问题。 资源有限的国际网站。总体而言，核心服务提供四个相辅相成的服务项目 包括以下步骤： 1)提供最先进的免疫学服务，以支持艾滋病毒研究和扩大 容量; 2)新的免疫学测定、技术和相关技术的评估和优化 为CFAR调查员提供服务; 3)在国际场所进行能力建设; 4)培训、指导和教育早期职业调查员、来自其他国家的高级调查员 学科和实验室工作人员。