Core E: Immunology Core

UCSF-Gladstone CFAR免疫学核心支持旨在改善 地方和全球艾滋病毒/艾滋病及相关合并症的临床管理和治疗， 并为制定有效的艾滋病毒治疗战略提供信息。免疫学核心使高度 通过提供获得免疫学专业知识的途径，合作进行多学科和转化性研究， 仪器和广泛的免疫学分析服务，包括多参数流动 免疫细胞表型和免疫功能(增殖、细胞毒性、细胞因子)的细胞学分析 生产和细胞信号)；骨髓、淋巴结和肠道的专门组织处理- 免疫磁珠法分离淋巴细胞亚群及相关淋巴组织 或对这些纯化细胞亚群中的下游信号通路进行流式细胞仪分类和分析。这个 CORE从两个不同的校园站点提供服务，包括CORE免疫学实验室 位于扎克伯格旧金山综合医院实验医学部和 Gladstone Flow Core位于观澜湾。在上一个资助期内，免疫学核心 撰写了115份同行评议的出版物，为45名研究员和初级研究员提供指导和培训 调查人员和15名实验室实习生。免疫学核心为流式细胞仪提供了访问 241个用户。根据CFAR调查人员不断变化的需求，中心推出了21个新的 分析和服务，并积极支持CFAR的科学主题，包括老龄化研究和共同 发病率；潜伏期和根除；以及国际研究。在接下来的五年里，免疫学 CORE将继续支持这些主题，并在当地和在 资源有限的国际站点。总体而言，核心服务提供四项补充服务 包括： 1)提供最先进的免疫学服务，以支持艾滋病毒研究和扩大 能力； 2)评估和优化新的免疫学检测、技术和相关技术 为CFAR调查人员提供的服务； 3)在国际场地建设能力；以及 4)培训、指导和教育职业生涯初期的调查员、来自其他国家的高级调查员 学科和实验室工作人员。

The UCSF-Gladstone CFAR Immunology Core supports immunology research aimed at improving the clinical management and treatment of HIV/AIDS and related co-morbidities, both locally and globally, and informing the development of an effective HIV cure strategy. The Immunology Core enables highly collaborative multidisciplinary and translational research by providing access to immunology expertise, instrumentation and a wide range of immunology assay services, including multiparameter flow cytometric assays of immune cell phenotype and immune function (proliferation, cytotoxicity, cytokine production and cell signaling); specialized tissue processing of bone marrow, lymph node and gut- associated lymphoid tissues plus isolation of specific subsets of lymphocytes by immunomagnetic bead or FACS sorting and analysis of downstream signaling pathways within these purified cell subsets. The Core provides services from two different campus sites including the Core Immunology Laboratory located within the Division of Experimental Medicine at Zuckerberg San Francisco General and the Gladstone Flow Core located at Mission Bay. During the last funding period, the Immunology Core contributed to 115 peer-reviewed publications, provided mentoring and training to 45 fellows and junior investigators and 15 laboratory interns. The Immunology Core has provided flow cytometer access to 241 users. The Core responded to changing needs of CFAR investigators by introducing 21 new assays and services and actively supported CFAR scientific themes including studies of aging and co- morbidity; latency and eradication; and international studies. Over the next five years, the Immunology Core will continue to support these themes, and respond to emerging questions both locally and at resource-limited international sites. Overall, the Core provides four complementary lines of service including: 1) The provision of state-of-the-art immunology services to support HIV research and expand capacity; 2) The evaluation and optimization of new immunological assays, technologies and related services for CFAR investigators; 3) Building capacity at international sites; and 4) Training, mentoring and education of early-career investigators, senior investigators from other disciplines, and laboratory staff.