Enhance AAV Liver Transduction with Capsid Immune Evasion

 产品说明：腺相关病毒（AAV）载体已成功应用于血友病B患者的肝靶向I期临床试验。然而，这些研究表明，AAV衣壳特异性细胞毒性T淋巴细胞（CTL）具有清除AAV转导的肝细胞的潜力，并导致治疗失败。我们先前的研究已经证明，AAV衣壳抗原呈递是剂量依赖性的，并且需要衣壳泛素化用于蛋白酶体介导的降解。AAV制备物中空病毒粒子的污染抑制来自AAV载体的完整颗粒的转导，并潜在地增加病毒衣壳抗原负载的风险。在这个提议中，我们将研究从AAV空病毒粒子的衣壳抗原呈递和空粒子对从完全病毒转导的抗原呈递的影响（目的1）。为了减少AAV转导细胞上的抗原呈递以避免衣壳特异性CTL介导的消除，已经提出修饰AAV衣壳表面或应用蛋白酶体抑制剂以增强AAV转导，同时降低有效剂量或逃避衣壳泛素化。我们将研究AAV突变体和蛋白酶体抑制剂对AAV衣壳抗原呈递的影响（目的2）。众所周知，AAV载体在小鼠模型中的转导并不总是翻译到人中。最后，我们将探索结合合理设计策略的定向进化方法，以分离具有人肝细胞特异性向性和在人源化小鼠中逃避衣壳特异性CTL应答的能力的AAV载体（目的3）。阐明体内AAV空衣壳抗原呈递和开发具有增强的人肝转导和CTL免疫逃避的AAV载体将使我们能够设计更安全和更有效的策略，以解决目前使用AAV的人肝基因治疗的临床并发症。

 DESCRIPTION: Adeno-associated virus (AAV) vector has been successfully applied in phase I clinical trials in hemophilia B patients with liver targeting. However, these studies have suggested that AAV capsid specific cytotoxic T lymphocytes (CTL) have the potential to eliminate AAV transduced hepatocytes and result in the therapeutic failure. Our prior studies have demonstrated that AAV capsid antigen presentation is dose-dependent and requires capsid ubiquitination for proteasome mediated degradation. The contamination of empty virions in AAV preparation inhibits transduction from full particles of AAV vectors and potentially increases the risk of virus capsid antigen load. In this proposal we will investigate capsid antige presentation from AAV empty virions and the effect of empty particles on antigen presentation from full virus transduction (Aim 1). To decrease antigen presentation on AAV transduced cells for avoiding capsid specific CTL-mediated elimination, it has been proposed to modify the AAV capsid surface or apply proteasome inhibitors to enhance AAV transduction while lowering the effective dose or to escape capsid ubiquitination. We will study the effect of AAV mutants and proteasome inhibitors on AAV capsid antigen presentation (Aim 2). It is well-known that the transduction of AAV vectors in mouse models does not always translate into the human. Finally, we will explore the directed evolution approach combined with a rational design strategy to isolate AAV vectors with human hepatocyte specific tropism and the ability to evade a capsid specific CTL response in humanized mice (Aim 3). Elucidation of AAV empty capsid antigen presentation in vivo and the development of an AAV vector with enhanced human liver transduction and CTL immune-evasion will allow us to design safer and more effective strategies that address the current clinical complications for human liver gene therapy using AAV.