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E-cigarettes and perturbations in the subgingival ecosystem

电子烟和龈下生态系统的扰动

基本信息

批准号：
9765271
负责人：
Purnima Kumar
金额：
$ 67.27万
依托单位：
OHIO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-16 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9765271
关键词：
Acute Address Adolescent and Young Adult Aerosols Affect Algorithmic Analysis Algorithms Animal Model Area Bacteria Bacterial Genes Bioinformatics Biological Biological Markers Biological Models Centers for Disease Control and Prevention (U.S.)Characteristics Child Chronic Cigarette Clinical Clinical Research Communities Custom Cytotoxic agent Data Disease Ecosystem Electronic Nicotine Delivery Systems Electronic cigarette Elements Entropy Equilibrium Event Exposure to Flavoring Formulation Gene Expression Gingival Crevicular Fluid Glycerol Goals Gold Graph Health Homeostasis Human Immune response Immunity In Vitro Individual Inflammatory Response Investigation Juice Knowledge Legal Maps Measures Metagenomics Methodology Mucous Membrane Nicotine Observational Study Oral Oral cavity Oral health Oral mucous membrane structure Outcome Pathogenicity Pathway Analysis Perception Periodontitis Phase Physiological Placebos Propylene Glycols Reporting Research Research Design Risk Risk Assessment Role Smoking Structure System Systems Biology Testing Time TimeLine Tobacco smoking behavior United States Validation Virulence arm base beta diversity catalyst clinical epidemiology cohort dosage dysbiosis electronic cigarette use electronic cigarette user high school host-microbe interactions immunogenic improved in vitro Model in vivo metatranscriptome metatranscriptomics microbial microbial host microbiome multilevel analysis mutualism nicotine replacement novel oral bacteria oral microbiome pathogen response smoking cessation subgingival microbiome tobacco products tool transcriptome transcriptome sequencing transcriptomics vaping young adult

项目摘要

Despite having been introduced ten years ago, little is known of the impact of electronic nicotine delivery systems (ENDS or e-cigarettes or e-cigs) on human health. ENDS usage is rapidly rising among adolescents and young adults and this is partially due to a perception that e-cigs are safer than cigarettes and other nicotine replacement therapies. However, these products vary widely in nicotine content, propylene glycol, glycerol, additives and flavorings; all of which are poorly studied. Therefore, there is an urgent need for biological investigations that will improve our understanding of how ENDS impact human health. The initial point of contact of ENDS, and the first-affected system in humans is the oral cavity. It is known that nicotine impacts oral health, acting as a cytotoxic agent as well as increasing virulence of certain bacteria. Oral health is dependent upon an intricate balance between the microbial ecosystem and host immunity. Based on our preliminary studies that ENDS alter the compositional structure and the functional potential of the oral ecosystem, we hypothesize that e-cigs will impact this microbial-mucosal homeostasis. We propose to test this hypothesis by combining novel clinical study designs with integrated `-omics' approaches and computational bioinformatics. This approach will bridge the gap between purely clinical outcome-based studies, in vitro investigations and in vivo investigations using animal models. First, the biological effects of different formulations of ENDS on an in vitro model of the oral host-microbial interactome will be examined through dual transcriptome-metatranscriptome sequencing. Second, we will observe the effects of ENDS on host-bacterial interactions over time by combining a multi-arm, longitudinal clinical study with a network analysis algorithm. The proposed studies will bring us closer to understanding the role of ENDS in disrupting oral ecological homeostasis, provide biologically-validated timelines for assessing risk associated with ENDS use, and develop indicators or predictors of exposure.

尽管电子尼古丁在十年前被引入，但人们对它的影响知之甚少