Flow Cytometry, Microscopy, and Imaging Core

流式细胞术、显微镜和成像核心

• 批准号: 9767819
• 负责人: Juhua Zhou
• 金额: $ 33.02万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9767819
• 关键词: Affect; Animals; Apoptosis; Biological Assay; Biological Process; Biotechnology; Blood Cells; Botanicals; Cell Proliferation; Cell physiology; Cells; Cellular Immunology; Centers of Research Excellence; Cessation of life; Color; Complex; Computer software; Confocal Microscopy; Consultations; Data; Data Analyses; Detection; Development; Disease; Disease Progression; Disease model; Educational workshop; Electron Microscopy; Endoscopes; Equipment; Equipment Design; Experimental Designs; Faculty; Flow Cytometry; Fluorescence; Fluorescence-Activated Cell Sorting; Funding; Goals; Grant; Group Meetings; Human Resources; Image; Image Analysis; Imaging Device; Immune System Diseases; Immunologics; Individual; Inflammation; Inflammation Process; Inflammatory; Label; Laboratories; Laboratory Animals; Lead; Microfluidics; Microscope; Microscopy; Modality; Molecular; Monitor; Optics; Organ; Organism; Pattern; Phase; Phenotype; Pilot Projects; Play; Population; Prevention; Prevention strategy; Price; Production; Program Evaluation; Proteins; Reader; Reproducibility; Research; Research Personnel; Resolution; Resource Sharing; Resources; Role; Sampling; Scanning; Services; Signal Pathway; Signaling Molecule; Sorting - Cell Movement; Structure; System; Technical Expertise; Techniques; Technology; Therapeutic; Time; Tissue imaging; Tissues; Training; Ultrasonography; United States Department of Veterans Affairs; United States National Institutes of Health; Work; base; biomarker development; cell determination; cell type; cellular imaging; cytokine; data acquisition; design; dietary supplements; early detection biomarkers; equipment training; experience; experimental study; high resolution imaging; image visualization; imaging facilities; imaging system; instrument; instrumentation; interest; medical schools; member; microscopic imaging; molecular marker; multiphoton microscopy; operation; response; submicron; therapeutic development; whole body imaging

Abstract The Flow Cytometry, Microscopy, and Imaging (FCMI) Core is a continuation from Phase-1 aimed at providing a wide range of biotechnology equipment and technical expertise to the COBRE junior faculty pursuing research on Dietary Supplements and Inflammation. The Core will provide state-of-the-art equipment to the Target and Pilot Project Faculty and their lab personnel for performing cutting-edge research in an accurate and timely manner that would lead to successful completion of their proposed projects. One of the focus of this Core is to provide multi-parameter flow cytometry and sorting services for phenotyping and isolation of specific cell types, determination of status on cell proliferation, differentiation, apoptosis as well as expression of signaling molecules and cytokines at the protein level in inflammation and following treatment with botanicals. The Core will also offer a range of imaging systems for live and fixed cells, tissues and organisms, including labeled and unlabeled samples. In addition, image visualization, data acquisition, processing and analysis that would meet the needs of the COBRE investigators are provided. Examination of structural changes that occur in response to inflammation, and how various dietary supplements induce alterations is critical to the understanding of the mechanisms of immune dysfunction, and development of therapeutic and preventive strategies. Specifically, the Core will provide imaging facilities for monitoring these changes at the whole animal to molecular level. This shared resource will lead to development of biomarkers for early detection and intermediate end points in the progression of the disease as well as response to therapeutic modalities. Furthermore, the instruments and expertise will provide specific and sensitive assay systems for determination of various molecular signaling pathways. At the whole animal level, ultrasound and fluorescence techniques are available to determine how inflammation and dietary supplements affect the experimental animals. The optical microscopy includes confocal and multiphoton microscopy for high resolution imaging of tissues to subcellular processes within tissues of interest. Finally, ultrastructural changes in cells will be imaged by electron microscopy to determine changes at the sub-micron level of resolution. A full range of image analysis hardware and software is also available to quantify the structural changes which occur during inflammation in the cells, tissues, and organs and how these changes are affected by the various dietary supplements. All equipment and technical expertise is available at USC SOM through outstanding institutional support. The Core faculty and staff will be available for assistance with experimental design, operation of equipment, and training on imaging instrumentation. In addition to individual interactions, didactic courses, seminars and workshops are regularly sponsored by the Instrumentation Resource Facility (IRF) at USC SOM, which will be made available to all members of the Target/Pilot Project Investigator laboratories.

摘要 流式细胞术、显微镜和成像（FCMI）核心是第1阶段的延续，旨在提供 广泛的生物技术设备和技术专长，以COBRE初级教师从事研究 关于膳食补充剂和炎症核心将为目标提供最先进的设备， 试点项目教师和他们的实验室人员进行准确和及时的前沿研究 这将有助于成功完成他们的项目。本核心的重点之一是 为特定细胞类型的表型分析和分离提供多参数流式细胞术和分选服务， 确定细胞增殖、分化、凋亡以及信号传导的表达的状态 分子和细胞因子在蛋白质水平的炎症和植物治疗后。核心 还将为活细胞和固定细胞、组织和生物体提供一系列成像系统，包括标记和 未标记的样品。此外，图像可视化，数据采集，处理和分析， 提供了COBRE调查员的需求。检查反应中发生的结构变化 炎症，以及各种膳食补充剂如何诱导改变是至关重要的理解， 免疫功能障碍的机制，以及治疗和预防策略的发展。具体地说， 核心将提供成像设备，用于在整个动物到分子水平上监测这些变化。这 共享资源将导致生物标志物的早期检测和中间终点的发展， 疾病的进展以及对治疗方式的反应。此外，文书和 专业知识将提供特定和灵敏的测定系统，用于测定各种分子信号传导 途径。在整个动物水平上，超声波和荧光技术可用于确定 炎症和膳食补充剂影响实验动物。光学显微镜包括共焦 和多光子显微镜，用于组织内的亚细胞过程的高分辨率成像， 兴趣最后，将通过电子显微镜对细胞的超微结构变化进行成像，以确定 亚微米级的分辨率。还提供全系列的图像分析硬件和软件， 量化炎症过程中细胞、组织和器官的结构变化，以及这些变化是如何发生的。 变化受到各种膳食补充剂的影响。所有设备和技术专长可在 USC SOM通过出色的机构支持。核心教师和工作人员将提供帮助 实验设计、设备操作和成像仪器培训。除了 个人互动，教学课程，研讨会和讲习班定期赞助的仪器 南加州大学SOM的资源设施（IRF），将向目标/试点项目的所有成员提供 研究者实验室。