Flow Cytometry, Microscopy, and Imaging Core

摘要 流式细胞术、显微镜和成像(FCMI)核心是第一阶段的延续，旨在提供 为从事研究的科布雷初级教员提供广泛的生物技术设备和技术专长 关于膳食补充剂和炎症。核心将向Target提供最先进的设备，并 试点项目教职员工及其实验室人员准确及时地开展前沿研究 这将导致成功完成其拟议的项目。这一核心的重点之一是 提供多参数流式细胞术和分选服务，用于特定细胞类型的表型和分离， 细胞增殖、分化、凋亡及信号表达状态的测定 炎症和植物药物治疗后蛋白质水平的分子和细胞因子。《核心》 还将为活的和固定的细胞、组织和生物体提供一系列成像系统，包括标记和 未标记的样本。此外，图像可视化、数据采集、处理和分析将满足 提供了科布雷调查人员的需求。审查应对措施所发生的结构性变化 对于炎症，以及各种膳食补充剂是如何引起变化的，对于理解 免疫功能障碍的机制，以及治疗和预防策略的发展。具体来说， 核心将提供成像设施，以监测这些变化在整个动物到分子水平。这 共享资源将导致开发用于早期检测的生物标记物和 疾病的进展以及对治疗方式的反应。此外，这些仪器和 专家将提供专门的和灵敏的分析系统来确定各种分子信号。 小路。在整个动物水平上，超声波和荧光技术可以用来确定 炎症和膳食补充剂会影响实验动物。光学显微镜包括共焦 和多光子显微镜，用于高分辨率成像组织到组织内的亚细胞过程 利息。最后，细胞的超微结构变化将通过电子显微镜进行成像，以确定在 亚微米级的分辨率。此外，还提供全方位的图像分析硬件和软件 量化炎症期间细胞、组织和器官中发生的结构变化，以及这些变化是如何 变化会受到各种膳食补充剂的影响。所有设备和技术专长均可在以下网址获得： 南加州大学高级管理学院通过杰出的机构支持。核心教职员工将随时提供帮助 具有实验设计、设备操作和成像仪器方面的培训。除了……之外 个人互动、授课课程、研讨会和工作坊定期由仪器公司赞助 南加州大学高级管理层的资源设施(IRF)，将向目标/试点项目的所有成员提供 调查员实验室。

Abstract The Flow Cytometry, Microscopy, and Imaging (FCMI) Core is a continuation from Phase-1 aimed at providing a wide range of biotechnology equipment and technical expertise to the COBRE junior faculty pursuing research on Dietary Supplements and Inflammation. The Core will provide state-of-the-art equipment to the Target and Pilot Project Faculty and their lab personnel for performing cutting-edge research in an accurate and timely manner that would lead to successful completion of their proposed projects. One of the focus of this Core is to provide multi-parameter flow cytometry and sorting services for phenotyping and isolation of specific cell types, determination of status on cell proliferation, differentiation, apoptosis as well as expression of signaling molecules and cytokines at the protein level in inflammation and following treatment with botanicals. The Core will also offer a range of imaging systems for live and fixed cells, tissues and organisms, including labeled and unlabeled samples. In addition, image visualization, data acquisition, processing and analysis that would meet the needs of the COBRE investigators are provided. Examination of structural changes that occur in response to inflammation, and how various dietary supplements induce alterations is critical to the understanding of the mechanisms of immune dysfunction, and development of therapeutic and preventive strategies. Specifically, the Core will provide imaging facilities for monitoring these changes at the whole animal to molecular level. This shared resource will lead to development of biomarkers for early detection and intermediate end points in the progression of the disease as well as response to therapeutic modalities. Furthermore, the instruments and expertise will provide specific and sensitive assay systems for determination of various molecular signaling pathways. At the whole animal level, ultrasound and fluorescence techniques are available to determine how inflammation and dietary supplements affect the experimental animals. The optical microscopy includes confocal and multiphoton microscopy for high resolution imaging of tissues to subcellular processes within tissues of interest. Finally, ultrastructural changes in cells will be imaged by electron microscopy to determine changes at the sub-micron level of resolution. A full range of image analysis hardware and software is also available to quantify the structural changes which occur during inflammation in the cells, tissues, and organs and how these changes are affected by the various dietary supplements. All equipment and technical expertise is available at USC SOM through outstanding institutional support. The Core faculty and staff will be available for assistance with experimental design, operation of equipment, and training on imaging instrumentation. In addition to individual interactions, didactic courses, seminars and workshops are regularly sponsored by the Instrumentation Resource Facility (IRF) at USC SOM, which will be made available to all members of the Target/Pilot Project Investigator laboratories.