NARMS: Enhance and Strengthen Antibiotic Resistance Surveillance in Retail Food Specimens

NARMS：加强零售食品样本中的抗生素耐药性监测

• 批准号: 9768153
• 负责人: Kirk Smith
• 金额: $ 15.32万
• 依托单位: MINNESOTA STATE DEPT OF HEALTH
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9768153
• 关键词: NARMS; Enhance; Strengthen; Antibiotic; Resistance

Project Summary: The Minnesota Department of Health (MDH) has been working with the Minnesota Department of Agriculture (MDA), CDC, and FDA on the Retail Food Study since its inception in 2002. This project was designed to determine the prevalence of antimicrobial resistance among enteric pathogens found in retail meats (including chicken, pork, ground beef, and ground turkey). In Minnesota, MDH and MDA are culturing all meats for Salmonella and chicken and ground turkey for Campylobacter. All isolates are further characterized by MDH or MDA and sent on to FDA for further analysis (including culture confirmation, antimicrobial susceptibility testing, and molecular subtyping). Historically, 40 samples (10 from each meat commodity) were tested each month. In May, 2015, with additional funding, we were able to increase the number of samples of chicken and ground turkey for a total of 80 meat samples per month (including pork and beef). Among the samples, all 80 are tested for Salmonella, all 20 turkey samples and 20 (half) of the chicken samples are tested for Campylobacter. In Minnesota, from 2002-2015, 184 of 1920 (9.6%) chicken samples, 222 of 1760 (12.6%) ground turkey samples, 10 of 1680 (0.6%) pork samples, and 10 of 1680 (0.6%) ground beef samples tested positive for Salmonella. Also in Minnesota from 2002-2015, 474 of 1760 (26.9%) chicken samples and 38 of 1760 (2.2%) ground turkey samples tested positive for Campylobacter. During 2002-2007, 1 of 720 (0.1%) pork samples, and 0 of 720 ground beef samples tested positive for Campylobacter. MDH and MDA plan to continue our cooperative arrangement. We will collect samples from the seven-county Minneapolis-St. Paul metropolitan area, which comprises over half of Minnesota’s population. This study has contributed substantial data to our understanding of the species, serotypes, and antimicrobial resistance types of Campylobacter and Salmonella found in various retail meats. This data has been beneficial in guiding our outbreak investigations where meats may have been the source of infection in humans. We have encountered very few barriers or challenges in our experience with this study and feel it has been a valuable tool, adding a critical component to the picture of antimicrobial resistance in Minnesota.

项目概要： 明尼苏达州卫生部（MDH）一直在与明尼苏达州卫生部 自2002年成立以来，农业部（MDA）、CDC和FDA一直在零售食品研究中发挥作用。这 项目旨在确定肠道菌群中抗生素耐药性的流行率， 零售肉类（包括鸡肉、猪肉、碎牛肉和火鸡肉）中发现的病原体。在 明尼苏达州，MDH和MDA正在培养沙门氏菌，鸡肉和火鸡肉 弯曲杆菌所有分离株进一步通过MDH或MDA表征，并送至FDA进行鉴定。 进一步分析（包括培养确认、抗菌药物敏感性测试，以及 分子分型）。从历史上看，40个样本（每种肉类商品10个）进行了测试， 月2015年5月，在额外的资金支持下，我们得以增加样本数量 每月抽取80个肉类样本（包括猪肉及 牛肉）。在这些样本中，所有80个样本都进行了沙门氏菌检测，所有20个火鸡样本和20个（一半） 测试鸡样品的弯曲杆菌。在明尼苏达州，从2002年至2015年，1920年的184 (9.6 1760份火鸡肉样品中有222份（12.6%），1680份猪肉样品中有10份（0.6%） 1680份碎牛肉样本中有10份（0.6%）沙门氏菌检测呈阳性。也 明尼苏达州从2002年至2015年，1760只鸡中的474只（26.9%）和1760只鸡中的38只（2.2%） 火鸡样本对弯曲杆菌呈阳性反应。2002-2007年期间，720份猪肉中有1份（0.1%） 720个碎牛肉样本中，0个样本对弯曲杆菌呈阳性反应。MDH和MDA 继续我们的合作安排我们将从七个县收集样本 明尼阿波利斯-圣保罗大都会区，包括明尼苏达州一半以上的人口。 这项研究为我们了解该物种、血清型和 在各种零售肉类中发现的弯曲杆菌和沙门氏菌的抗菌素耐药性类型。 这一数据有助于指导我们对肉类可能存在的疫情进行调查。 人类的传染源在我们的工作中，我们遇到的障碍或挑战很少。 我对这项研究的经验，并认为它是一个有价值的工具，增加了一个关键组成部分， 明尼苏达州抗菌素耐药性的图片。