A conditional and rapid cell ablation technology in zebrafish

斑马鱼条件快速细胞消融技术

• 批准号: 9896887
• 负责人: Anand Chandrasekhar
• 金额: $ 7.49万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-04-01 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9896887
• 关键词: Ablation; Affect; Binding; Binding Sites; Biological Models; Blood Circulation; Cell Death; Cell membrane; Cell physiology; Cells; Cephalic; Cytolysis; Data; Development; Developmental Process; Disadvantaged; Dose; Embryo; Event; Exhibits; Genetic; Hour; Human; In Vitro; Incubated; Investigative Techniques; Larva; Letters; Mammals; Mediating; Methods; Monitor; Motor Neurons; Neurobiology; Neurons; Pathogenesis; Penetration; Permeability; Physiology; Process; Proteins; RNA; Reporter; Research; Sodium Chloride; Specificity; Streptococcus intermedius; Swimming; System; Technology; Temperature; Testing; Tissues; Toxin; Transgenic Organisms; Validation; Work; Zebrafish; cell type; cold temperature; developmental disease; experimental study; genetic technology; in vivo; jaw movement; neuronal cell body; novel; receptor; surfactant; tissue regeneration

PROJECT SUMMARY Targeted cell ablation is a powerful approach for investigating cellular functions, tissue regeneration, developmental processes and disease pathogenesis. Several genetic methods are available for cell ablation, but the ablation process is relatively slow, requiring at least several hours. This can be a disadvantage when examining relatively rapid cellular events (ranging from seconds to minutes) during development or in physiology. This proposal tests the feasibility of a novel genetic cell ablation technology established in mammals for mediating rapid cell ablation in zebrafish. Preliminary data indicate that the system works very well in vitro with cultured zebrafish neurons. These studies will optimize the conditions for effective and rapid cell ablation in vivo, in gastrulating embryos to swimming larvae.

项目摘要 靶向细胞消融是研究细胞功能、组织再生 发育过程和疾病发病机制。几种遗传方法可用于细胞消融， 但是烧蚀过程相对缓慢，需要至少几个小时。这可能是一个缺点， 检查发育过程中或发育中相对快速的细胞事件（从几秒到几分钟不等）， physiology.这项提议测试了一种新的基因细胞消融技术的可行性， 哺乳动物介导的快速细胞消融在斑马鱼。初步数据表明，该系统的工作非常 在体外培养的斑马鱼神经元中表现良好。这些研究将优化条件， 细胞消融在体内，在原肠胚游泳幼虫。