Cell cycle regulation in response to plasma membrane stress in S. cerevisae
Cell cycle regulation in response to plasma membrane stress in S. cerevisae
批准号:
9897602
负责人:
Amy E Ikui
金额:
$35.33万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2017
资助国家:
美国
项目状态:
已结题
起止时间:
2017-04-01 至 2021-03-31
关键词:
Biological AssayCDK2 geneCell CycleCell Cycle ArrestCell Cycle CheckpointCell Cycle RegulationCell WallCell membraneCell surfaceCellsChromosomal InstabilityChromosomesConsensusConsensus SequenceCyclin-Dependent Kinase InhibitorCyclin-Dependent KinasesCyclinsDNADNA biosynthesisEukaryotaEukaryotic CellFailureFluorescence MicroscopyG1 PhaseGene ExpressionGene ProteinsGenesGlycogen Synthase Kinase 3GoalsGrowthHumanIn VitroLeadLinkMalignant NeoplasmsMembraneMitogen-Activated Protein Kinase KinasesMitogen-Activated Protein KinasesMolecularMonomeric GTP-Binding ProteinsOrganismPRKCA genePathway interactionsPhenotypePhosphorylationPhosphorylation SitePhosphotransferasesPlasma CellsPopulation GrowthProtein DephosphorylationProtein Kinase CProteinsSignal TransductionSiteStressStructureTestingTranscriptional ActivationTwo-Hybrid System TechniquesYeastsbiological adaptation to stresscell growthenvironmental changemutantnovelpathogenpolarized cellpreventresponsesensortime usetumorigenesis
中文摘要
项目摘要/摘要
酵母细胞需要快速控制其细胞周期、基因表达或蛋白质
应激反应通路激活时的稳定性。对于细胞来说,
他们的质膜和细胞壁受到各种攻击,如物理破坏,
病原体入侵和各种环境扰动。在这里,我们建议研究
质膜应激通过一种新的细胞周期抑制细胞生长和DNA复制
检查点路径。真核细胞复制它们的染色体。DNA中的错误
复制控制可能导致染色体不稳定,这是癌症的一个标志。我们的长-
学期目标是了解DNA复制控制的分子机制
压力。
在这个提案中,三个目标将检验我的假设,如果质膜损伤
通过一种新的细胞周期检查点抑制极化的细胞生长和DNA复制
路径。
Aim1将确定膜损伤如何驱动Pkc1重新定位以抑制极化细胞
成长。
AIM2将决定质膜应激信号如何激活Mpk1以抑制DNA
复制。
Aim3将确定酵母GSK-3激酶Mck1是否针对血浆上的复制蛋白
膜应力
从酵母到人类,DNA复制和膜完整性途径都被很好地保存了下来。
本研究的结果可应用于理解黄曲霉毒素的分子机制。
高等真核生物对应激的DNA复制调控。
英文摘要
Project Summary/Abstract
Yeast cells need to rapidly control their cell cycle profile, gene expression or protein
stability in response to the activation of stress response pathways. It is common for cells to
suffer from various attacks to their plasma membrane and cell wall, such as physical damage,
pathogen invasion and various environmental perturbations. Here we propose to study
that plasma membrane stress inhibits cell growth and DNA replication through a novel cell cycle
checkpoint pathway. Eukaryotic cells duplicate their chromosomes. Errors in the DNA
replication control could lead to chromosome instability which is a hallmark of cancer. Our long-
term goal is to understand the molecular mechanism of DNA replication control in response to
stress.
In this proposal, three aims will test my hypothesis if plasma membrane damage
inhibits polarized cell growth and DNA replication through a novel cell cycle checkpoint
pathway.
Aim1 will determine how membrane damage drives Pkc1 relocation to inhibit polarized cell
growth.
Aim2 will determine how plasma membrane stress signal activates Mpk1 to inhibit DNA
replication.
Aim3 will determine if a yeast GSK-3 kinase Mck1 targets replication proteins upon plasma
membrane stress
DNA replication and membrane integrity pathways are well conserved from yeast to humans.
The results obtained from this study can be applied to understand the molecular mechanism of
DNA replication control in response to stress in higher eukaryotes.
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会议论文
Cell cycle regulation in response to plasma membrane stress in S. cerevisae
-
批准号:9209454
-
项目类别:
-
资助金额:$36.69万
-
财政年份:2017
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负责人:Amy E Ikui
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依托单位:
A role of PP2A-Cdc55 in cell cycle
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项目类别:
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财政年份:2017
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负责人:Amy E Ikui
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依托单位:
A role of PP2A-Cdc55 in cell cycle
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批准号:10378659
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资助金额:$39.25万
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依托单位:
A role of PP2A-Cdc55 in cell cycle
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批准号:10171487
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项目类别:
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资助金额:$39.25万
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Inhibition of DNA re-replication by cyclin/CDK and GSK-3 kinase in S. cerevisiae
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批准号:8690114
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项目类别:
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依托单位:
Inhibition of DNA re-replication by cyclin/CDK and GSK-3 kinase in S. cerevisiae
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批准号:8473647
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项目类别:
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资助金额:$11.78万
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财政年份:2013
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依托单位:
Inhibition of DNA re-replication by cyclin/CDK and GSK-3 kinase in S. cerevisiae
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批准号:9040204
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项目类别:
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资助金额:$11.78万
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财政年份:2013
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负责人:Amy E Ikui
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依托单位:
A novel function of Orc6 during mitosis in S. cerevisiae
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批准号:8241917
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项目类别:
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资助金额:$15.54万
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财政年份:2010
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依托单位:
A novel function of Orc6 during mitosis in S. cerevisiae
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批准号:8051808
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项目类别:
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资助金额:$15.54万
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财政年份:2010
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负责人:Amy E Ikui
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依托单位:
A novel function of Orc6 during mitosis in S. cerevisiae
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批准号:7852515
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项目类别:
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资助金额:$15.7万
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财政年份:2010
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负责人:Amy E Ikui
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依托单位:
海外基金