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Regulation and Consequences of Ets1 Downregulation in B Cells

B 细胞中 Ets1 下调的调控和后果

基本信息

批准号：
9896768
负责人：
LEE ANN Garrett-Sinha
金额：
$ 52.41万
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-04-01 至 2022-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9896768
关键词：
1-Phosphatidylinositol 3-Kinase 3&apos Untranslated Regions Address Affect Alleles Antibodies Area Autoantibodies Autoantigens Autoimmune Diseases Autoimmune Process Autoimmunity B cell differentiation B-Cell Activation B-Lymphocytes Biological Assay CD22 gene CRISPR/Cas technology Cell Nucleus Cell Surface Receptors Development Discrimination Disease Down-Regulation Event Failure Foundations Genes Genetic Polymorphism Genetic Transcription Human Immune response Immune system Immunization Inflammation Knock-out Lead Light Link Luciferases Lupus MAPK8 gene Maintenance Mediating Modeling Molecular Mus NR0B2 gene Pathogenicity Pathway interactions Patients Phosphoric Monoester Hydrolases Plasma Cells Process Production Protein Tyrosine Kinase Proteins Receptor Signaling Receptors, Antigen, B-Cell Regulation Reporter Signal Pathway Signal Transduction Signaling Protein Specificity Susceptibility Gene Symptoms Systemic Lupus Erythematosus Testing Tissues Toll-like receptors Up-Regulation experimental study genome wide association study genomic locus immune function insight novel strategies novel therapeutic intervention pathogen plasma cell differentiation prevent protein degradation public health relevance recruit sialic acid binding Ig-like lectin transcription factor

项目摘要

DESCRIPTION (provided by applicant): B cells can be activated to differentiate into antibody-secreting plasma cells (PCs), a process crucial for normal immune function. However, unregulated B cell differentiation can lead to the production of autoantibodies and the development of autoimmune diseases such as systemic lupus erythematosus (SLE). Therefore, B cell differentiation is under tight control. Stimulation of B cells via the B cell receptor (BCR)or Toll-like receptors (TLRs) induces activation, proliferation and differentiation by influencing the expression and activity of transcription factors in the nucleus of the B cell. Counterbalancing these activation pathways are inhibitory signaling pathways dependent on a number of cell surface receptors (for example, CD22 and Siglec-G) that are phosphorylated by the tyrosine kinase Lyn. This results in the recruitment of phosphatases such as SHP1 that reverse activation of signaling proteins in the BCR and TLR cascades. Better understanding of the negative and positive pathways controlling B cell differentiation will provide new clues into how they might be manipulated to limit B cell activation in autoimmune diseases. We have recently identified the transcription factor Ets1 to be a crucial target that is downregulated by positive BCR or TLR signaling, and whose expression is maintained by inhibitory signaling cascades. Because Ets1 blocks B cell differentiation to plasma cells, its regulation is a key event in determining the fate of B cells upon stimulation. In the absence of Ets1 or Lyn, which maintains Ets1 expression, mice accumulate plasma cells and autoantibodies. Therefore maintenance of appropriate Ets1 levels in B cells is important to prevent autoimmunity. In this proposal we will define the causes and consequences of Ets1 downregulation in B cells. In Aim 1, we will further characterize the mechanisms controlling Ets1 expression in B cells. In Aim 2, we will determine the consequences of failure to downregulate Ets1 in B cells for both humoral immune responses and the development of autoimmunity using mice in which Ets1 can be inducibly expressed in B cells. In Aim 3, we will determine whether Ets1 is similarly downregulated by activating signals in primary human B cells, whether SLE- associated Ets1 polymorphisms affect control of Ets1 expression, and whether B cells from SLE patients demonstrate reduced Ets1 expression or more efficient Ets1 downregulation. Taken together, these studies will further our understanding of the molecular mechanisms of B cell differentiation and may reveal novel therapeutic approaches for diseases such as SLE.