Cell atlas of mouse brain-spinal cord connectome

小鼠脑脊髓连接组细胞图谱

基本信息

  • 批准号:
    9768566
  • 负责人:
  • 金额:
    $ 233.69万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2018
  • 资助国家:
    美国
  • 起止时间:
    2018-09-01 至 2023-05-31
  • 项目状态:
    已结题

项目摘要

PROJECT SUMMARY Although great efforts have been dedicated to characterizing neuronal cell types in the brain, systematic studies on the brain-spinal cord connectome and associated spinal neuronal types are lacking. In this project, a team of seven laboratories proposes to use a highly innovative and multidisciplinary approach to systematically characterize neuronal types in the spinal cord based on their anatomy, connectivity, neuronal morphologies, molecular identities, and electrophysiological properties. In Aim 1, we will use a newly developed AAV anterograde transsynaptic tagging method to label spinal cord neurons that receive descending inputs from different brain regions, and use a retrograde viral tracer, AAVretro, to label spinal neurons that project to defined brain regions. These tagged neurons will be imaged in the intact whole spinal cord with a newly developed fast 3D light sheet microscopy technique, and targeted for recording in slice preparations. The axonal collateral patterns, dendritic morphologies, and electrophysiological properties will be compared between different input/output-defined spinal neuron groups. In Aim 2, the gene expression patterns of the tagged neurons will be determined in situ by sequential bar-coded FISH (seqFISH), with candidate marker genes obtained from online resources, or from single-cell sorting and RNA sequencing (Dropseq). In Aim 3, all collected data on connectivity, anatomical cell type distribution map, neuronal morphologies, molecular identities, and electrophysiological properties will be used for classifying spinal neuron types connected with brain, and an open-source data portal will be established which will allow users to search, view, and analyze the multi-modal and integrative cell-type specific data. Together, we aim to construct a comprehensive cell- type atlas of the mouse brain-spinal cord connectome.
项目总结

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Hong-Wei Dong其他文献

Hong-Wei Dong的其他文献

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{{ truncateString('Hong-Wei Dong', 18)}}的其他基金

A three dimensional multimodal cellular connectivity atlas of the mouse hypothalamus
小鼠下丘脑三维多模态细胞连接图谱
  • 批准号:
    10719606
  • 财政年份:
    2023
  • 资助金额:
    $ 233.69万
  • 项目类别:
Sexual dimorphic cell type and connectivity atlases of the aging and AD mouse brains
衰老和 AD 小鼠大脑的性二态性细胞类型和连接图谱
  • 批准号:
    10740308
  • 财政年份:
    2023
  • 资助金额:
    $ 233.69万
  • 项目类别:
Mapping Cellular Resolution Connectopathies in Aging and Alzheimer's Disease
绘制衰老和阿尔茨海默氏病的细胞分辨率连接病图谱
  • 批准号:
    10431675
  • 财政年份:
    2022
  • 资助金额:
    $ 233.69万
  • 项目类别:
Mapping Cellular Resolution Connectopathies in Aging and Alzheimer's Disease
绘制衰老和阿尔茨海默氏病的细胞分辨率连接病图谱
  • 批准号:
    10621814
  • 财政年份:
    2022
  • 资助金额:
    $ 233.69万
  • 项目类别:
Next-generation MORF Mice for Scalable Brainwide Morphological Mapping and Genetic Perturbation of Single Neurons
下一代 MORF 小鼠,用于可扩展的全脑形态映射和单神经元的遗传扰动
  • 批准号:
    10370248
  • 财政年份:
    2021
  • 资助金额:
    $ 233.69万
  • 项目类别:
The Mouse Connectome Project Phase III: Assembling the global neural networks of the mouse brain
小鼠连接组项目第三阶段:组装小鼠大脑的全局神经网络
  • 批准号:
    10226677
  • 财政年份:
    2020
  • 资助金额:
    $ 233.69万
  • 项目类别:
Dendritome mapping of genetically-defined and sparsely-labeled cortical and striatal projection neurons
遗传定义和稀疏标记的皮质和纹状体投射神经元的树突状图谱
  • 批准号:
    10407481
  • 财政年份:
    2018
  • 资助金额:
    $ 233.69万
  • 项目类别:
Dendritome mapping of genetically-defined and sparsely-labeled cortical and striatal projection neurons
遗传定义和稀疏标记的皮质和纹状体投射神经元的树突状图谱
  • 批准号:
    10171916
  • 财政年份:
    2018
  • 资助金额:
    $ 233.69万
  • 项目类别:
Cell atlas of mouse brain-spinal cord connectome
小鼠脑脊髓连接组细胞图谱
  • 批准号:
    9583948
  • 财政年份:
    2018
  • 资助金额:
    $ 233.69万
  • 项目类别:
Dendritome mapping of genetically-defined and sparsely-labeled cortical and striatal projection neurons
遗传定义和稀疏标记的皮质和纹状体投射神经元的树突状图谱
  • 批准号:
    9768581
  • 财政年份:
    2018
  • 资助金额:
    $ 233.69万
  • 项目类别:

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