Collaborative Standards for Brain Microscopy

项目摘要/摘要 快速显微技术，再加上组织清除方面的最新进展，现在能够有效地 制作完整大脑样本的细胞分辨率图像。这些技术已经产生了三个- 来自模型生物和人脑大切片的完整大脑的三维(3D)图像 样本，能够映射从突触到系统水平的神经元电路。展望未来，这将是 在实验室之间共享数据以复制研究结果、执行交叉研究分析和开发 强大的新分析工具。该应用程序的目标是为神经科学研究社区提供 拥有一套完整大脑的3D显微镜标准。这些标准将有助于数据的比较 以及复制和验证结果以及解决新的研究问题所需的集成， 通过推进创新神经技术®(大脑)计划扩大大脑研究 对技术开发的投资。我们的方法将是建立一个敏捷工作组(WG)， 并利用协商一致的过程来建立脑显微镜的协作标准。一起， 我们经验丰富的多学科团队将建立和传播完整大脑的3D显微镜 通过以下具体目标制定标准。具体目标1：确定项目运作参数 并召集一个由3D脑显微镜专家组成的工作组。RTI-UNC团队将与国家 心理健康研究所(NIMH)项目科学家、工作组联络人和其他利益相关者聚集在一起 不同的科学家群体，他们认识到该项目的愿景和影响，并有足够的 科学地位和专业知识，以代表社区和解决范围。具体目标2：发展 初步标准采用协商一致的过程，依赖于研究界的投入。 工作组将开发完整大脑实验的3D显微镜标准，使用动态的、协作的 协商一致的过程。工作组将首先利用实际的实验参数制定一个原型标准， 数据和元数据。初步标准随后将与研究社区共享，以获得 工作组在最后确定一套标准时将考虑的反馈和意见。特定的 目标3：提高人们对3D脑显微镜标准的认识和理解。RTI-UNC团队 将使用多方面的方法来确保这些标准是有价值的、有用的和广泛传播的。 我们将开发一个可浏览、可搜索的基于Web的资源来托管和推广这些标准， 与调查人员合作实施这些标准，发布同行评议的手稿，请访问 会议，与专业协会互动，鼓励使用标准，并鼓励期刊 促进标准在出版物中的使用。

PROJECT SUMMARY/ABSTRACT Fast microscopy techniques, coupled with recent advances in tissue clearing, are now able to efficiently produce cellular-resolution images of intact brain samples. These technologies have generated three- dimensional (3D) images of entire intact brains from model organisms and large sections of human brain samples, enabling mapping of neuronal circuits from synapse to systems levels. Going forward, it will be essential to share data across laboratories to replicate findings, perform cross-study analyses, and develop robust new analysis tools. The goal of this application is to provide the neuroscience research community with a set of standards for 3D microscopy of intact brains. These standards will facilitate the data comparison and integration necessary for replicating and validating results and for addressing new research questions, amplifying the Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative's investment in technology development. Our approach will be to establish an agile Working Group (WG) of experts and to use a consensus process to establish collaborative standards for brain microscopy. Together, our experienced, multidisciplinary team will establish and disseminate 3D microscopy of intact brain standards through the following specific aims. Specific Aim 1: Establish project operating parameters and assemble a WG of 3D brain microscopy experts. The RTI-UNC team will work with the National Institute of Mental Health (NIMH) project scientist, WG Liaisons, and other stakeholders to assemble a diverse group of scientists who recognize the vision and impact of the project and who have sufficient scientific stature and expertise to represent the community and address the scope. Specific Aim 2: Develop preliminary standards using a consensus process that relies on input from the research community. The WG will develop standards for 3D microscopy of intact brain experiments using a dynamic, collaborative consensus process. The WG will first develop a prototype standard using actual experimental parameters, data, and metadata. The preliminary standards will then be shared with the research community to get feedback and comments that will be considered by the WG as they finalize the set of standards. Specific Aim 3: Raise awareness and promote uptake of 3D brain microscopy standards. The RTI-UNC team will use a multifaceted approach to ensure that the standards are valuable, useful, and widely disseminated. We will develop a browsable, searchable, Web-based resource to host and promote the standards, collaborate with investigators to implement the standards, publish peer-reviewed manuscripts, present at conferences, interact with professional societies to encourage use of the standards, and encourage journals to promote use of the standards in publications.