Collaborative Standards for Brain Microscopy

项目概要/摘要 快速的显微镜技术，加上组织清除的最新进展，现在能够有效地 生成完整大脑样本的细胞分辨率图像。这些技术产生了三个- 来自模型生物体的整个完整大脑和人脑的大切片的三维（3D）图像 样本，使神经元电路从突触映射到系统水平。展望未来， 跨实验室共享数据以复制研究结果、进行交叉研究分析并开发 强大的新分析工具。该应用程序的目标是为神经科学研究社区提供 一套完整大脑的3D显微镜标准。这些标准将便于数据比较 和整合所必需的复制和验证结果，并解决新的研究问题， 通过推进创新神经技术（BRAIN）计划扩大大脑研究 投资于技术开发。我们的方法是建立一个敏捷工作组（WG）， 专家，并使用共识的过程，建立合作标准的脑显微镜。在一起， 我们经验丰富的多学科团队将建立和传播完整大脑的3D显微镜 通过以下具体目标。具体目标1：确定项目运行参数 组建一个3D脑显微镜专家工作组RTI-TBI团队将与国家 精神卫生研究所（NIMH）项目科学家，工作组联络员和其他利益相关者， 认识到该项目的愿景和影响力，并有足够的 科学地位和专业知识，以代表社区和解决的范围。目标二：发展 初步标准使用的共识过程，依赖于研究界的投入。 工作组将制定标准的三维显微镜完整的大脑实验使用动态，协作 协商一致进程。工作组将首先使用实际的实验参数开发一个原型标准， 数据和元数据。初步标准将与研究界分享， 工作组在最后确定这套标准时将考虑的反馈和意见。具体 目标3：提高认识并促进3D脑显微镜标准的采用。RTI-TVB团队 将采用多方面的方法，以确保这些标准是有价值的、有用的和广泛传播的。 我们将开发一个可浏览、可搜索、基于网络的资源，以托管和推广这些标准， 与研究人员合作实施标准，发表同行评审的手稿，出席 会议，与专业协会互动，鼓励使用标准，并鼓励期刊 促进在出版物中使用这些标准。

PROJECT SUMMARY/ABSTRACT Fast microscopy techniques, coupled with recent advances in tissue clearing, are now able to efficiently produce cellular-resolution images of intact brain samples. These technologies have generated three- dimensional (3D) images of entire intact brains from model organisms and large sections of human brain samples, enabling mapping of neuronal circuits from synapse to systems levels. Going forward, it will be essential to share data across laboratories to replicate findings, perform cross-study analyses, and develop robust new analysis tools. The goal of this application is to provide the neuroscience research community with a set of standards for 3D microscopy of intact brains. These standards will facilitate the data comparison and integration necessary for replicating and validating results and for addressing new research questions, amplifying the Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative's investment in technology development. Our approach will be to establish an agile Working Group (WG) of experts and to use a consensus process to establish collaborative standards for brain microscopy. Together, our experienced, multidisciplinary team will establish and disseminate 3D microscopy of intact brain standards through the following specific aims. Specific Aim 1: Establish project operating parameters and assemble a WG of 3D brain microscopy experts. The RTI-UNC team will work with the National Institute of Mental Health (NIMH) project scientist, WG Liaisons, and other stakeholders to assemble a diverse group of scientists who recognize the vision and impact of the project and who have sufficient scientific stature and expertise to represent the community and address the scope. Specific Aim 2: Develop preliminary standards using a consensus process that relies on input from the research community. The WG will develop standards for 3D microscopy of intact brain experiments using a dynamic, collaborative consensus process. The WG will first develop a prototype standard using actual experimental parameters, data, and metadata. The preliminary standards will then be shared with the research community to get feedback and comments that will be considered by the WG as they finalize the set of standards. Specific Aim 3: Raise awareness and promote uptake of 3D brain microscopy standards. The RTI-UNC team will use a multifaceted approach to ensure that the standards are valuable, useful, and widely disseminated. We will develop a browsable, searchable, Web-based resource to host and promote the standards, collaborate with investigators to implement the standards, publish peer-reviewed manuscripts, present at conferences, interact with professional societies to encourage use of the standards, and encourage journals to promote use of the standards in publications.