Collaborative Standards for Brain Microscopy

脑显微镜协作标准

• 批准号: 9768588
• 负责人: Bruce Wayne Huggins
• 金额: $ 52.39万
• 依托单位: RESEARCH TRIANGLE INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9768588
• 关键词: 3-Dimensional; Address; Animal Model; Awareness; Behavior; Brain; Brain Mapping; Brain region; Cells; Common Data Element; Communities; Computer software; Consensus; Coupled; Data; Data Analyses; Data Collection; Data Files; Development; Dictionary; Dimensions; Disease; Ensure; Feedback; Frozen Sections; Funding; Goals; Human; Image; Individual; International; Investments; Journals; Knowledge; Label; Laboratories; Letters; Light; Manuscripts; Metadata; Microscopy; Molecular; Mus; National Institute of Mental Health; Neurosciences; Neurosciences Research; North Carolina; Pathology; Preparation; Process; Professional Organizations; Protocols documentation; Publications; Published Comment; Publishing Peer Reviews; Rattus; Research; Research Methodology; Research Personnel; Resolution; Sampling; Scientist; Standardization; Synapses; System; Techniques; Technology; Three-Dimensional Image; Time; Tissues; Universities; Vision; Work; Zebrafish; brain research; cell type; connectome; data format; data management; data modeling; data sharing; experience; experimental study; file format; flexibility; hackathon; innovative neurotechnologies; member; movie; multidisciplinary; neuronal circuitry; online resource; prototype; repository; symposium; technology development; three dimensional structure; tool; uptake; usability; working group

PROJECT SUMMARY/ABSTRACT Fast microscopy techniques, coupled with recent advances in tissue clearing, are now able to efficiently produce cellular-resolution images of intact brain samples. These technologies have generated three- dimensional (3D) images of entire intact brains from model organisms and large sections of human brain samples, enabling mapping of neuronal circuits from synapse to systems levels. Going forward, it will be essential to share data across laboratories to replicate findings, perform cross-study analyses, and develop robust new analysis tools. The goal of this application is to provide the neuroscience research community with a set of standards for 3D microscopy of intact brains. These standards will facilitate the data comparison and integration necessary for replicating and validating results and for addressing new research questions, amplifying the Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative's investment in technology development. Our approach will be to establish an agile Working Group (WG) of experts and to use a consensus process to establish collaborative standards for brain microscopy. Together, our experienced, multidisciplinary team will establish and disseminate 3D microscopy of intact brain standards through the following specific aims. Specific Aim 1: Establish project operating parameters and assemble a WG of 3D brain microscopy experts. The RTI-UNC team will work with the National Institute of Mental Health (NIMH) project scientist, WG Liaisons, and other stakeholders to assemble a diverse group of scientists who recognize the vision and impact of the project and who have sufficient scientific stature and expertise to represent the community and address the scope. Specific Aim 2: Develop preliminary standards using a consensus process that relies on input from the research community. The WG will develop standards for 3D microscopy of intact brain experiments using a dynamic, collaborative consensus process. The WG will first develop a prototype standard using actual experimental parameters, data, and metadata. The preliminary standards will then be shared with the research community to get feedback and comments that will be considered by the WG as they finalize the set of standards. Specific Aim 3: Raise awareness and promote uptake of 3D brain microscopy standards. The RTI-UNC team will use a multifaceted approach to ensure that the standards are valuable, useful, and widely disseminated. We will develop a browsable, searchable, Web-based resource to host and promote the standards, collaborate with investigators to implement the standards, publish peer-reviewed manuscripts, present at conferences, interact with professional societies to encourage use of the standards, and encourage journals to promote use of the standards in publications.

项目总结/文摘