Toward a human adult brain cell atlas with single-cell technologies

摘要 人脑是一个极其复杂的空间组织和功能网络 嵌入神经胶质细胞中的连接神经元。超过小鼠大脑三个数量级 就细胞的绝对数量而言，其数量级很大，并且可能具有更复杂的组织 与人类特定认知功能相关的结构。定义完整的细胞图谱 人脑，包括所有细胞类型（即所有部分）及其空间分布的完整目录， 是理解人类认知机器的关键一步。 在这个项目中，我们将大大扩展我们之前的努力，以建立一个完整的、 人类全脑的空间分辨细胞图谱，使用三种新颖的组合 可扩展的单细胞测序和原位成像技术。新颖的计算 还将开发对这些更大规模的数据进行整合和分析的方法。 我们将系统地将这些方法应用于 MRI 扫描的人类全脑，以产生 参考女性和男性大脑的细胞图，并构建空间多组学图 人类成人的大脑。

Abstract Human brain is an exceedingly complex network of spatially organized and functionally connected neurons imbedded in glia. In surpasses the mouse brain by three orders of magnitude in terms of sheer numbers of cells, and likely has a more complex organization structure relevant to human-specific cognitive functions. Defining a complete cell atlas of the human brain, including a full catalog of all cell types (i.e. all parts) and their spatial distribution, is a critical step towards understanding the human cognitive machine. In this project we will greatly expand our previous efforts towards building a complete and spatially resolved cell atlas of the human whole brain, using a combination of three novel technologies for scalable single-cell sequencing and in situ imaging. Novel computational approaches will also be developed for integration and analysis of data at these larger scales. We will systematically apply these methods to MRI-scanned human whole brains to produce reference cell maps for female and male brains, and construct a spatial multi-omic map of the human adult brain.