Swarming motility and the regulation of flagellar biosynthesis in Bacillus subtilis
枯草芽孢杆菌的集群运动和鞭毛生物合成的调控
基本信息
- 批准号:9898391
- 负责人:
- 金额:$ 48.98万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-04-01 至 2024-03-31
- 项目状态:已结题
- 来源:
- 关键词:AnabolismArchitectureBacillus subtilisBacteriaBasic ScienceBiochemistryCell surfaceCellsComplexEnvironmentFlagellaGoalsGram-Positive BacteriaLearningLengthLocationMediatingMicroscopyOctodonPathogenesisPatternPeptidoglycanPilumPositioning AttributeProteinsProteolysisRegulationResolutionRodSolidStimulusSurfaceSystemThickTimeUpdateVirulenceWorkcell envelopecell growthcell motilityforward geneticsnanomachineresponseself organizationspatiotemporal
项目摘要
ABSTRACT
Many bacteria are motile by synthesizing corkscrew-like flagella which when rotated propel bacteria
through the environment. Each bacterium synthesizes a species-specific number of flagella and inserts the
flagella in a species-specific pattern on the cell surface. Flagella are complex nanomachines assembled from
dozens of different proteins and how each bacterial species controls flagellar number and patterning is poorly-
understood. Moreover, the number of flagella per cell increases when cells come into contact a solid surface to
initiate a form of surface motility called swarming. The Kearns lab uses classical forward genetics, super-
resolution microscopy, and biochemistry to study flagellar biosynthesis and swarming motility of the Gram
positive bacterium Bacillus subtilis. The goals of the project are to understand flagellar biosynthesis in the
context of growing cell architecture. First, we will determine how flagellar number is controlled by the poorly-
understood master regulator of flagellar biosynthesis SwrA and a response regulator DegU. Second, we will
explore how the surface contact response is transduced to inhibit the adaptor-mediated regulatory proteolysis
of SwrA and increase flagellar number. Third, we will learn how the flagellar rod insertion through
peptidoglycan occurs, and how rod length is controlled to match the thickness of peptidoglycan. Fourth, flagella
are synthesized in a grid-like pattern and we will study how flagellar patterning is interpreted and updated in
time during cell growth, and coordinated with peptidoglycan insertion. Ultimately, we want to achieve a holistic
understanding of how a cell dynamically governs the initiation of flagellar biosynthesis at specific locations to
insert the machine through the cell envelope. Our basic research is fundamental to how cells self-organize and
is applicable to the spatiotemporal control of the assembly of transenvelope nanomachines involved in
pathogenesis including flagella, pili and secretion systems like the injectisome.
摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Daniel B Kearns其他文献
Daniel B Kearns的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Daniel B Kearns', 18)}}的其他基金
Swarming motility and the regulation of flagellar biosynthesis in Bacillus subtilis
枯草芽孢杆菌的集群运动和鞭毛生物合成的调控
- 批准号:
10370420 - 财政年份:2019
- 资助金额:
$ 48.98万 - 项目类别:
Swarming motility and the regulation of flagellar biosynthesis in Bacillus subtilis
枯草芽孢杆菌的集群运动和鞭毛生物合成的调控
- 批准号:
10582609 - 财政年份:2019
- 资助金额:
$ 48.98万 - 项目类别:
Structural homeostasis and the assembly of a bacterial nanomachine
结构稳态和细菌纳米机器的组装
- 批准号:
9098739 - 财政年份:2010
- 资助金额:
$ 48.98万 - 项目类别:
The mechanism of bistable motility gene expression in Bacillus subtilis.
枯草芽孢杆菌双稳态运动基因表达机制。
- 批准号:
8294679 - 财政年份:2010
- 资助金额:
$ 48.98万 - 项目类别:
The mechanism of bistable motility gene expression in Bacillus subtilis.
枯草芽孢杆菌双稳态运动基因表达机制。
- 批准号:
8496079 - 财政年份:2010
- 资助金额:
$ 48.98万 - 项目类别:
The mechanism of bistable motility gene expression in Bacillus subtilis.
枯草芽孢杆菌双稳态运动基因表达机制。
- 批准号:
7861445 - 财政年份:2010
- 资助金额:
$ 48.98万 - 项目类别:
The mechanism of bistable motility gene expression in Bacillus subtilis.
枯草芽孢杆菌双稳态运动基因表达机制。
- 批准号:
8678945 - 财政年份:2010
- 资助金额:
$ 48.98万 - 项目类别:
The mechanism of bistable motility gene expression in Bacillus subtilis.
枯草芽孢杆菌双稳态运动基因表达机制。
- 批准号:
8111249 - 财政年份:2010
- 资助金额:
$ 48.98万 - 项目类别:
相似海外基金
CAREER: Efficient Algorithms for Modern Computer Architecture
职业:现代计算机架构的高效算法
- 批准号:
2339310 - 财政年份:2024
- 资助金额:
$ 48.98万 - 项目类别:
Continuing Grant
Hardware-aware Network Architecture Search under ML Training workloads
ML 训练工作负载下的硬件感知网络架构搜索
- 批准号:
2904511 - 财政年份:2024
- 资助金额:
$ 48.98万 - 项目类别:
Studentship
CAREER: Creating Tough, Sustainable Materials Using Fracture Size-Effects and Architecture
职业:利用断裂尺寸效应和架构创造坚韧、可持续的材料
- 批准号:
2339197 - 财政年份:2024
- 资助金额:
$ 48.98万 - 项目类别:
Standard Grant
Travel: Student Travel Support for the 51st International Symposium on Computer Architecture (ISCA)
旅行:第 51 届计算机体系结构国际研讨会 (ISCA) 的学生旅行支持
- 批准号:
2409279 - 财政年份:2024
- 资助金额:
$ 48.98万 - 项目类别:
Standard Grant
Understanding Architecture Hierarchy of Polymer Networks to Control Mechanical Responses
了解聚合物网络的架构层次结构以控制机械响应
- 批准号:
2419386 - 财政年份:2024
- 资助金额:
$ 48.98万 - 项目类别:
Standard Grant
I-Corps: Highly Scalable Differential Power Processing Architecture
I-Corps:高度可扩展的差分电源处理架构
- 批准号:
2348571 - 财政年份:2024
- 资助金额:
$ 48.98万 - 项目类别:
Standard Grant
Collaborative Research: Merging Human Creativity with Computational Intelligence for the Design of Next Generation Responsive Architecture
协作研究:将人类创造力与计算智能相结合,设计下一代响应式架构
- 批准号:
2329759 - 财政年份:2024
- 资助金额:
$ 48.98万 - 项目类别:
Standard Grant
The architecture and evolution of host control in a microbial symbiosis
微生物共生中宿主控制的结构和进化
- 批准号:
BB/X014657/1 - 财政年份:2024
- 资助金额:
$ 48.98万 - 项目类别:
Research Grant
NSF Convergence Accelerator Track M: Bio-Inspired Surface Design for High Performance Mechanical Tracking Solar Collection Skins in Architecture
NSF Convergence Accelerator Track M:建筑中高性能机械跟踪太阳能收集表皮的仿生表面设计
- 批准号:
2344424 - 财政年份:2024
- 资助金额:
$ 48.98万 - 项目类别:
Standard Grant
RACCTURK: Rock-cut Architecture and Christian Communities in Turkey, from Antiquity to 1923
RACCTURK:土耳其的岩石建筑和基督教社区,从古代到 1923 年
- 批准号:
EP/Y028120/1 - 财政年份:2024
- 资助金额:
$ 48.98万 - 项目类别:
Fellowship














{{item.name}}会员




