Turning a sequence barcode into a spectral barcode for single-cell analysis.

将序列条形码转换为光谱条形码以进行单细胞分析。

基本信息

批准号：
9898410
负责人：
ILYA J FINKELSTEIN
金额：
$ 18.19万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-04-01 至 2022-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9898410
关键词：
Address Architecture Bar Codes Bioinformatics Biology Bypass Cell Nucleus Cells Code Color Complementary DNA Cytoplasm DNA DNA Probes DNA Sequence Data Data Analyses Databases Detection Development Developmental Biology Encapsulated Fluorescence Fluorescent Probes Genetic Transcription Genomics Goals Hybrids Hydrogels Image Individual Knowledge Libraries Light Machine Learning Maps Mechanics Metals Methods Microfluidic Microchips Microfluidics Microscope Microscopy Mining Molecular Molecular Analysis Motivation Nature Nuclear Nucleic Acids Nucleotides Phenotype Physical assessment Process Reading Reporter Research Reverse Transcription Series Silver Specificity Stretching Techniques Technology Testing base cell community cost design developmental disease droplet sequencing experimental study fluorescence imaging interest multiplex detection nanocluster nanoparticle next generation nucleobase screening single cell analysis technology development tool transcriptome transcriptomics

项目摘要

Project Summary: The use of sequence barcodes has enabled high-throughput transcriptomic analysis of single cells. But one challenge remains – there is no method to map the physical assessments of single cells and the downstream transcriptomic data of single cells to the same cells of origin. This is because currently sequence barcodes are only read by sequencing which takes place after all single cells are lysed, reverse transcription is completed, and cDNA are amplified and pooled. In order to perform transcriptomic analysis and physical assessments on the same single cells, we will need a method that allows us to decipher the sequence barcodes while in the process of single-cell physical interrogation. Our goal in this proposed research is to develop a new method to turn sequence barcodes into spectral barcodes that can be read locally in the process. The proposed sequence- barcode-reading technique, if it can be realized, will have substantial impact to the single-cell community as it will become the only method to map the physical assessments and the downstream molecular analysis data to the same cells of origin in a high-throughput, streamlined format.

项目摘要：序列条形码的使用已实现了单个细胞的高通量转录组分析。但是一个挑战仍然存在 - 没有方法可以映射单细胞和下游的物理评估单细胞到相同原点细胞的转录组数据。这是因为当前序列条形码为仅通过测序读取所有单个单元裂解后进行的，逆转录完成，和cDNA被放大和合并。为了进行转录组分析和物理评估相同的单个单元格，我们将需要一种方法，使我们能够在单细胞物理询问的过程。我们在这项拟议的研究中的目标是开发一种新方法将序列条形码转换为光谱条形码，这些条形码可以在此过程中局部读取。提出的序列 - 条形码阅读技术（如果可以实现）将对单细胞社区产生重大影响将成为将物理评估和下游分子分析数据映射到的唯一方法具有高通量，简化格式的原始单元。