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Turning a sequence barcode into a spectral barcode for single-cell analysis.

将序列条形码转换为光谱条形码以进行单细胞分析。

基本信息

批准号：
9898410
负责人：
ILYA J FINKELSTEIN
金额：
$ 18.19万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-04-01 至 2022-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9898410
关键词：
Address Architecture Bar Codes Bioinformatics Biology Bypass Cell Nucleus Cells Code Color Complementary DNA Cytoplasm DNA DNA Probes DNA Sequence Data Data Analyses Databases Detection Development Developmental Biology Encapsulated Fluorescence Fluorescent Probes Genetic Transcription Genomics Goals Hybrids Hydrogels Image Individual Knowledge Libraries Light Machine Learning Maps Mechanics Metals Methods Microfluidic Microchips Microfluidics Microscope Microscopy Mining Molecular Molecular Analysis Motivation Nature Nuclear Nucleic Acids Nucleotides Phenotype Physical assessment Process Reading Reporter Research Reverse Transcription Series Silver Specificity Stretching Techniques Technology Testing base cell community cost design developmental disease droplet sequencing experimental study fluorescence imaging interest multiplex detection nanocluster nanoparticle next generation nucleobase screening single cell analysis technology development tool transcriptome transcriptomics

项目摘要

Project Summary: The use of sequence barcodes has enabled high-throughput transcriptomic analysis of single cells. But one challenge remains – there is no method to map the physical assessments of single cells and the downstream transcriptomic data of single cells to the same cells of origin. This is because currently sequence barcodes are only read by sequencing which takes place after all single cells are lysed, reverse transcription is completed, and cDNA are amplified and pooled. In order to perform transcriptomic analysis and physical assessments on the same single cells, we will need a method that allows us to decipher the sequence barcodes while in the process of single-cell physical interrogation. Our goal in this proposed research is to develop a new method to turn sequence barcodes into spectral barcodes that can be read locally in the process. The proposed sequence- barcode-reading technique, if it can be realized, will have substantial impact to the single-cell community as it will become the only method to map the physical assessments and the downstream molecular analysis data to the same cells of origin in a high-throughput, streamlined format.

项目摘要：序列条形码的使用使得单细胞的高通量转录组学分析成为可能。但有一挑战仍然存在-没有方法来映射单个细胞和下游细胞的物理评估，将单个细胞的转录组学数据与来源相同的细胞进行比较。这是因为目前序列条形码是仅通过在所有单细胞裂解后进行的测序读取，完成逆转录，扩增并汇集cDNA。为了进行转录组学分析和身体评估，同样的单细胞，我们将需要一种方法，使我们能够破译序列条形码，而在单细胞物理审讯过程。我们在这项研究中的目标是开发一种新的方法，将序列条形码转换为可以在该过程中本地读取的光谱条形码。建议的顺序- 条形码读取技术，如果它能够实现，将对单细胞群落产生重大影响，因为它将成为映射物理评估和下游分子分析数据的唯一方法，以高通量、流线型的形式收集相同来源的细胞。