Cell diversity and organization in the medial superior olive

项目摘要 广泛影响：Meal Superior Olive(MSO)检测声音到达时间的差异 两只耳朵计算声音位置(耳间时间差，ITD)。MSO神经元高度专门化 来检测这些微秒级的时序差异。虽然MSO能够检测简单声音的ITD， 例如音调，是众所周知的，但很少有人研究更多的ITD如何 检测到复杂/自然主义的声音。我们实验室的初步数据显示，有几组 MSO中的神经元具有不同于典型的“主要”MSO神经元的生理特性。这个 这项提议的首要目标是发现这些新细胞在声音定位中的作用 体内和体外技术相结合。目标1A：进行体内贴片实验，以确定 新的细胞类型的功能作用，记录它们在不同声音刺激下的反应。目标 1B：在脑片上进行体外斑贴实验，以表征这些新细胞类型和 将这些属性与已知的主要单元格属性进行比较。目标2：使用依赖活性的病毒标记 检查这些新细胞类型的组织结构的技术。这些实验将揭示这些 先前描述的神经元已经在声音定位电路中并且大大扩展了该领域的 了解MSO如何处理声音定位。培训：这个项目将教给申请者 几项新技术，包括体内膜片钳，使用病毒工具，以及组织学/成像。此外， 申请者将有机会参加与其他实验室的本地和国际合作。 这项提议将有助于申请人通过会议向科学界传播他的工作。 演讲和期刊出版物，以及为他在学术领域的未来提供技能。

Project Summary Broad Impact: The Medial Superior Olive (MSO) detects differences in the arrival time of sounds between the two ears to compute the sounds location (Interaural time difference, ITD). MSO neurons are highly specialized to detect these microsecond differences in timing. While the MSO's ability to detect ITDs of simple sounds, such as tones, is well understood, little work has been done investigating how ITDs of more complex/naturalistic sounds are detected. Preliminary data from our lab shows that there are groups of neurons in the MSO with different physiological properties from typical “principal” MSO neurons. The overarching goal of this proposal is to discover the roles of these new cells in sound localization using a combination of in vivo and in vitro techniques. Aim 1A: Perform in vivo patch experiments to determine the functional role of new cell types by recording their responses when different sound stimuli are presented. Aim 1B: in vitro patch experiments in brain slices to characterize the synaptic properties of these new cell types and to compare these properties to known principal cell properties. Aim 2: Use activity-dependent viral labeling techniques to examine the organization of these new cell types. These experiments will reveal the role these previously described neurons have in the sound localization circuit and greatly expand the field's understanding of how the MSO processes sound location. Training: This project will teach the applicant several new techniques, including in vivo patch clamp, using viral tools, and histology/imaging. Additionally, the applicant will have the opportunity to participate in both local and international collaborations with other labs. This proposal will aid the applicant in disseminating his work to the scientific community through conferences presentations and journal publications, as well as provide him with skills for his future in the academic field.