ShEEP Equipment Request for BD FACSMelody Cell Sorter

ShEEP BD FACSMelody 细胞分选仪设备请求

• 批准号: 9906433
• 负责人: Hubert T. Kim
• 金额: --
• 依托单位: VETERANS AFFAIRS MED CTR SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9906433
• 关键词: Area; Biological Sciences; Cell Separation; Cells; Cellular biology; Collaborations; Communities; Endocrinology; Equipment; Fluorescence-Activated Cell Sorting; Funding; Funding Agency; Goals; Healthcare; Healthcare Systems; Home environment; Mission; Musculoskeletal Diseases; Neurology; Operative Surgical Procedures; Orthopedics; Patients; Research; Research Personnel; Sampling; San Francisco; Schedule; Services; Sheep; Site; System; United States National Institutes of Health; cost; experimental study; improved; instrument; wasting

This application is to acquire an BD FACS Melody Cell Sorting System (BD Biosciences) on behalf of the San Francisco VA Health Care System (SFVAHCS) to support researchers at Mission Bay campus to conduct cell sorting in their researches funded by VA and other federal and non-federal funding sources. SFVAHCS Mission Bay (MB) campus is the research home for over 20 investigators at SFVAHCS. Cell biology is common research area for those researchers. Fluorescence-activated cell sorting (FACS) is essential equipment for cell isolation, which has becoming a routine experiment for many investigators at SFVAHCS-MB campus. However, there is no FACS cell sorter available on this site. Long distance between MB and Ft. Miley main campuses of SFVAHCS makes it unrealistic for investigators at MB to use FACS at Ft. Miley campus. Although nearby UCSF FACS core is open to VA researchers, that facility is serving hundreds of UCSF users with very busy schedule. Many of our experiments were cancelled or delayed due to the unavailability of UCSF FACS facility, resulting in wasting of many valuable samples. This new BD FACS Melody Cell Sorting System homed at SFVAHCS MB campus will support 11 VA investigators at MB campus with 7 active VA funded projects and 8 more NIH and DoD projects initially, facilitating and accelerating their research to improve VA health care.

本申请是为了获得BD FACS Melody细胞分选系统（BD Biosciences），代表 旧金山弗朗西斯科卫生保健系统（SFVAHCS）支持研究人员在使命湾校园进行 细胞分选在他们的研究资助的VA和其他联邦和非联邦资金来源。SFVAHCS 使命湾（MB）校园是SFVAHCS 20多名调查人员的研究基地。细胞生物学是 这些研究人员的共同研究领域。流式细胞术（FACS）是一种重要的 细胞分离设备，这已成为SFVAHCS-MB许多研究人员的常规实验 校园但是，该网站上没有可用的FACS细胞分选仪。MB和Ft之间的距离很远。麦莉 SFVAHCS的主要校区使得MB的研究人员在Ft使用FACS是不现实的。麦莉校园 虽然附近的加州大学旧金山分校流式细胞仪核心是开放给弗吉尼亚州的研究人员，该设施是服务于数百名加州大学旧金山分校的用户 非常忙碌的时间表。我们的许多实验被取消或延迟由于UCSF的不可用 FACS设备，导致浪费了许多有价值的样品。这款新的BD FACS Melody细胞分选系统 位于SFVAHCS MB校园，将支持MB校园的11名VA调查员，其中7名活动VA资助 项目和8个NIH和国防部项目，促进和加速他们的研究，以提高VA 保健