ShEEP Request for ImageXpress Pico Automated Cell Imaging System

ShEEP 请求 ImageXpress Pico 自动细胞成像系统

• 批准号: 9906104
• 负责人: THOMAS A. RANDO
• 金额: --
• 依托单位: VETERANS ADMIN PALO ALTO HEALTH CARE SYS
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9906104
• 关键词: 3-Dimensional; Address; Alzheimer' s Disease; Apoptosis; Autoimmunity; Biological Assay; Cell Count; Cell model; Cells; Cellular Assay; Computer software; Degenerative polyarthritis; Disease; Ensure; Environmental Monitoring; Evaluation; Fluorescence Microscopy; Fracture; Generations; Gulf War; Image; Image Analysis; Imaging Device; Individual; Infection; Intuition; Medical Research; Microscope; Microscopic; Mitochondria; Neurites; Optics; Proteins; Protocols documentation; Pulmonary Hypertension; Research; Running; Scientist; Series; Sheep; Slice; Slide; System; Technology; Time; Traumatic Brain Injury; Veterans; Work; base; cellular imaging; chronic pain; detector; experimental study; fluorescence imaging; imaging system; instrument; live cell imaging; neuroinflammation; operation; programs; real time monitoring; tool; volumetric muscle loss

The ImageXpress Pico Automated Cell Imaging System combines cutting-edge technology with quality and exceptional ease of operation. The new system features outstanding sensitivity, thanks to highly corrected optics and the efficiency of its detectors and electronic components. Due to intuitive software and excellent optics, the ImageXpress Pico system allows for efficient high-throughput imaging and analysis of various cellular assays and can be programed to take photos over multiple slides and in microplates from 6 well to 384-well. We will be able to expand our assay capabilities with over 25 preconfigured templates optimized for cell-based experiments including apoptosis, mitochondrial evaluation, 3D cell models, live cell/time lapse, and neurite tracing. ImageXpress Pico system offers objectives ranging from 4x to 63x and can operate in colorimetric, brightfield, or fluorescence imaging modes. It includes preconfigured analysis protocols ranging from cell counting to sophisticated neurite tracing analysis, which removes the guess work from optimizing parameters. Z-stack acquisition enables the generation of sharper images and more accurate segmentation and a series of images acquired at different focal points captures more details than with an individual slice. Multi-day, time lapse, and live cell assays can be run on the system. The software provides real-time monitoring of environmental state, ensuring optimal assay conditions. The CellReporterXpress package is easy to use and is suitable for classical fluorescence microscopy and special applications alike, such as live cell imaging and image analysis. All components included in our request and attached quote are integral parts of the ImageXpress Pico Automated Cell Imaging System and will not be used independently.

ImageXpress Pico 自动细胞成像系统将尖端技术与质量和 操作极其方便。新系统具有出色的灵敏度，得益于高度校正 光学及其探测器和电子元件的效率。由于直观的软件和出色的 光学方面，ImageXpress Pico 系统可实现高效的高通量成像和各种分析 细胞分析，并且可以编程在多个载玻片和微孔板中从 6 孔到 384-井。我们将能够通过超过 25 个针对以下情况进行优化的预配置模板来扩展我们的检测能力 基于细胞的实验，包括细胞凋亡、线粒体评估、3D 细胞模型、活细胞/时间推移以及 神经突追踪。 ImageXpress Pico 系统提供 4 倍至 63 倍范围内的物镜，并且可以在 比色、明场或荧光成像模式。它包括预配置的分​​析协议 从细胞计数到复杂的神经突追踪分析，消除了优化过程中的猜测工作 参数。 Z 堆栈采集能够生成更清晰的图像和更准确的分割 在不同焦点获取的一系列图像比单个切片捕获更多细节。 系统上可以运行多天、延时和活细胞分析。该软件提供实时 监测环境状态，确保最佳的测定条件。 CellReporterXpress 软件包很简单 使用并适用于经典荧光显微镜和特殊应用，例如活细胞 成像和图像分析。我们的请求和所附报价中包含的所有组件都是 ImageXpress Pico 自动细胞成像系统，不会独立使用。