Hypercalciuria and TRPV6-mediated Active Calcium Reabsorption in the Proximal Tubule
高钙尿症和 TRPV6 介导的近端小管主动钙重吸收
基本信息
- 批准号:9899982
- 负责人:
- 金额:$ 33.41万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-07-25 至 2022-03-31
- 项目状态:已结题
- 来源:
- 关键词:Angiotensin IIAnimalsApicalBackCalciumCalcium ChannelCellsCompensatory HyperinsulinemiaCrystal FormationDevelopmentDiabetes MellitusDiseaseDistalEventGoalsHenle&aposs loopHomeostasisHumanHydrochlorothiazideHyperinsulinismInsulinInsulin ResistanceKidneyKidney CalculiKnockout MiceLiquid substanceMediatingMixed Function OxygenasesMolecularMonitorMusNephronsOutcomePathway interactionsPharmaceutical PreparationsPhysiologicalPlasmaPopulationPrediabetes syndromeProductionPublic HealthResearchRiskRoleSideSignal Transduction PathwayTestingThiazide DiureticsTubular formationVitamin DWatercalcium excretioncalcium phosphatediagnostic biomarkerhuman subjecthypercalciuriainsulin secretionkidney cellmetabolic abnormality assessmentnephrogenesisnovelpreventsensortherapeutic targetthiazideurinarywasting
项目摘要
PROJECT SUMMARY
Hypercalciuria occurs in 5-10% of the population and is the most common cause of calcium (Ca) kidney stone
disease. Ca reabsorption takes place in different nephron segments; however, evidence from human subjects
indicates that proximal tubule Ca reabsorption is decreased in stone formers. Furthermore, studies in both
animals and humans suggest that increased proximal tubule Ca reabsorption accounts for the hypocalciuric
effect of thiazide diuretics, the first line medication for hypercalciuria. Compared to Ca reabsorption in the distal
tubule, much less is known about the mechanism of Ca reabsorption in the proximal tubule. Lines of evidence
from classical physiological studies indicate the existence of an active Ca transport pathway in the proximal
straight tubule, the last portion of the proximal tubule where Ca can be reabsorbed before tubular fluid enters the
loop of Henle where supersaturation of Ca phosphate may occur due to water extraction. Active Ca reabsorption
in this segment of the nephron may remove Ca in the tubular fluid to a level below that in the plasma. The
objective of this proposal is to elucidate the roles of a novel active Ca transport pathway in the proximal straight
tubule in causing and treating hypercalciuria. The central hypothesis is that active Ca reabsorption in the
proximal tubule is disrupted by postprandial insulin surge and under hyperinsulinemia and is enhanced by
thiazides. In addition, a Ca channel in the apical side of proximal straight tubule may act as a Ca sensor to
regulate overall Ca homeostasis. It has been shown that postprandial insulin secretion and Ca excretion are both
increased in hypercalciuric stone formers. Our preliminary studies indicate that TRPV6, a key Ca channel in the
transcellular Ca transport pathway, is distributed to the proximal straight tubule, and TRPV6 activity is inhibited by
insulin, and the hypocalciuric effect of hydrochlorothiazide is significantly reduced in TRPV6 null mice. These
observations support our hypothesis, which implies that inhibition of TRPV6 by insulin contributes to postprandial
hypercalciuria and hypercalciuria due to compensatory hyperinsulinemia as a result of insulin resistance. We will
test our hypothesis and achieve our research objective by pursuing the following specific aims. 1. Determine the
inhibitory effect of insulin on active Ca reabsorption in the proximal tubule. 2. Determine to what extent TRPV6
mediates the hypocalciuric effect of thiazides. 3. Determine the Ca-sensor roles of TRPV6 in the proximal
straight tubule that regulate vitamin D synthesis and paracellular Ca transport. Approaches ranging from Ca
influx in human proximal tubule cells to metabolic studies using TRPV6 null mice will be employed to accomplish
our research goals. Upon successful completion of the proposed research, we expect to unveil the molecular
mechanism for the active Ca reabsorption in the proximal tubule that regulates Ca reabsorption and Ca
homeostasis. The inhibition of the active Ca reabsorption by insulin provides a molecular basis for the
postprandial hypercalciuria in many stone formers and elevated Ca excretion in prediabetes. These outcomes
will help to develop a new strategy to prevent hypercalciuria and kidney stone disease.
项目摘要
高钙尿症发生在5-10%的人口,是最常见的原因钙(钙)肾结石
疾病钙重吸收发生在不同的肾单位段;然而,来自人类受试者的证据
表明结石形成者近端小管钙重吸收减少。此外,两项研究
动物和人类表明,近端小管钙重吸收增加,
噻嗪类利尿剂的作用,高钙尿症的一线药物。与远端的钙重吸收相比,
近端小管钙重吸收的机制尚不清楚。条证据
从经典的生理学研究表明,存在一个积极的钙转运途径,在近端
直小管,近端小管的最后一部分,在小管液体进入前钙可以被重吸收。
亨利环,由于水提取,可能会发生磷酸钙过饱和。活性钙重吸收
在肾单位的这一部分中,可以将管状流体中的Ca去除到低于血浆中的水平。的
本研究的目的是阐明近端直肌中一种新的主动钙转运途径的作用,
肾小管在引起和治疗高钙尿症中的作用。中心假设是,在细胞中的活性钙重吸收,
近端小管被餐后胰岛素峰和高胰岛素血症破坏,
噻嗪类。此外,近端直小管顶侧的钙通道可能作为钙传感器,
调节整体Ca稳态。已经表明,餐后胰岛素分泌和钙排泄都是
在高钙尿石形成者中增加。我们的初步研究表明,TRPV 6,一个关键的钙通道,在细胞膜上,
在跨细胞Ca转运途径中,TRPV 6分布到近端直小管,TRPV 6活性被
在TRPV 6缺失小鼠中,氢氯噻嗪的低钙尿效应显著降低。这些
观察结果支持我们的假设,这意味着胰岛素对TRPV 6的抑制有助于餐后
高钙尿和由于胰岛素抵抗引起的代偿性高胰岛素血症引起的高钙尿。我们将
通过追求以下具体目标来验证我们的假设并实现我们的研究目标。1.确定
胰岛素对近曲小管活性钙重吸收的抑制作用。2.确定TRPV 6在多大程度上
介导噻嗪类的低钙尿效应。3.确定TRPV 6在近端细胞中的Ca传感器作用
调节维生素D合成和细胞旁钙转运直小管。方法包括:
将采用人近端小管细胞中的流入来进行使用TRPV 6缺失小鼠的代谢研究,
我们的研究目标。在成功完成拟议的研究后,我们预计将揭开分子
近端小管中活性钙重吸收的机制,调节钙重吸收和钙
体内平衡胰岛素对活性钙重吸收的抑制作用,为钙离子的释放提供了分子基础。
许多结石形成者餐后高钙尿症和糖尿病前期钙排泄升高。这些结果
将有助于开发一种新的策略来预防高钙尿症和肾结石疾病。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JI-BIN PENG其他文献
JI-BIN PENG的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JI-BIN PENG', 18)}}的其他基金
Hypercalciuria and TRPV6-mediated Active Calcium Reabsorption in the Proximal Tubule
高钙尿症和 TRPV6 介导的近端小管主动钙重吸收
- 批准号:
9308401 - 财政年份:2017
- 资助金额:
$ 33.41万 - 项目类别:
Hypercalciuria and TRPV6-mediated Active Calcium Reabsorption in the Proximal Tubule
高钙尿症和 TRPV6 介导的近端小管主动钙重吸收
- 批准号:
10132304 - 财政年份:2017
- 资助金额:
$ 33.41万 - 项目类别:
Interplay of Renal Ca and Na Transport Pathways
肾钙和钠转运途径的相互作用
- 批准号:
7990200 - 财政年份:2009
- 资助金额:
$ 33.41万 - 项目类别:
Interplay of Renal Ca and Na Transport Pathways
肾钙和钠转运途径的相互作用
- 批准号:
7585652 - 财政年份:2007
- 资助金额:
$ 33.41万 - 项目类别:
Regulation of WNK4, a protein kinase mutated in a hereditary form of hypertension
WNK4(一种在遗传性高血压中发生突变的蛋白激酶)的调节
- 批准号:
9248355 - 财政年份:2007
- 资助金额:
$ 33.41万 - 项目类别:
Regulation of WNK4, a protein kinase mutated in a hereditary form of hypertension
WNK4(一种在遗传性高血压中发生突变的蛋白激酶)的调节
- 批准号:
8694189 - 财政年份:2007
- 资助金额:
$ 33.41万 - 项目类别:
Interplay of Renal Ca and Na Transport Pathways
肾钙和钠转运途径的相互作用
- 批准号:
7765825 - 财政年份:2007
- 资助金额:
$ 33.41万 - 项目类别:
Interplay of Renal Ca and Na Transport Pathways
肾钙和钠转运途径的相互作用
- 批准号:
7362397 - 财政年份:2007
- 资助金额:
$ 33.41万 - 项目类别:
Interplay of Renal Ca and Na Transport Pathways
肾钙和钠转运途径的相互作用
- 批准号:
8044037 - 财政年份:2007
- 资助金额:
$ 33.41万 - 项目类别:
Regulation of WNK4, a protein kinase mutated in a hereditary form of hypertension
WNK4(一种在遗传性高血压中发生突变的蛋白激酶)的调节
- 批准号:
9040151 - 财政年份:2007
- 资助金额:
$ 33.41万 - 项目类别:
相似海外基金
The earliest exploration of land by animals: from trace fossils to numerical analyses
动物对陆地的最早探索:从痕迹化石到数值分析
- 批准号:
EP/Z000920/1 - 财政年份:2025
- 资助金额:
$ 33.41万 - 项目类别:
Fellowship
Animals and geopolitics in South Asian borderlands
南亚边境地区的动物和地缘政治
- 批准号:
FT230100276 - 财政年份:2024
- 资助金额:
$ 33.41万 - 项目类别:
ARC Future Fellowships
The function of the RNA methylome in animals
RNA甲基化组在动物中的功能
- 批准号:
MR/X024261/1 - 财政年份:2024
- 资助金额:
$ 33.41万 - 项目类别:
Fellowship
Ecological and phylogenomic insights into infectious diseases in animals
对动物传染病的生态学和系统发育学见解
- 批准号:
DE240100388 - 财政年份:2024
- 资助金额:
$ 33.41万 - 项目类别:
Discovery Early Career Researcher Award
Zootropolis: Multi-species archaeological, ecological and historical approaches to animals in Medieval urban Scotland
Zootropolis:苏格兰中世纪城市动物的多物种考古、生态和历史方法
- 批准号:
2889694 - 财政年份:2023
- 资助金额:
$ 33.41万 - 项目类别:
Studentship
Using novel modelling approaches to investigate the evolution of symmetry in early animals.
使用新颖的建模方法来研究早期动物的对称性进化。
- 批准号:
2842926 - 财政年份:2023
- 资助金额:
$ 33.41万 - 项目类别:
Studentship
Study of human late fetal lung tissue and 3D in vitro organoids to replace and reduce animals in lung developmental research
研究人类晚期胎儿肺组织和 3D 体外类器官在肺发育研究中替代和减少动物
- 批准号:
NC/X001644/1 - 财政年份:2023
- 资助金额:
$ 33.41万 - 项目类别:
Training Grant
RUI: Unilateral Lasing in Underwater Animals
RUI:水下动物的单侧激光攻击
- 批准号:
2337595 - 财政年份:2023
- 资助金额:
$ 33.41万 - 项目类别:
Continuing Grant
RUI:OSIB:The effects of high disease risk on uninfected animals
RUI:OSIB:高疾病风险对未感染动物的影响
- 批准号:
2232190 - 财政年份:2023
- 资助金额:
$ 33.41万 - 项目类别:
Continuing Grant
A method for identifying taxonomy of plants and animals in metagenomic samples
一种识别宏基因组样本中植物和动物分类的方法
- 批准号:
23K17514 - 财政年份:2023
- 资助金额:
$ 33.41万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)