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RNA scanning by regulatory endonucleases - Resubmission - 1

通过调节核酸内切酶进行 RNA 扫描 - 重新提交 - 1

基本信息

批准号：
9901585
负责人：
JOEL G BELASCO
金额：
$ 40.04万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-04-06 至 2022-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9901585
关键词：
3-Dimensional Address Affect Amino Acids Bacteria Bacterial Genome Base Pairing Binding Binding Sites Biochemical Genetics Biological Biology Catalytic Domain Cells Characteristics Complementary RNA Distant Ensure Enzymes Escherichia coli Excision Gene Expression Gene Expression Regulation Genes Genetic Transcription Goals Homologous Gene Human Knowledge Medical Messenger RNA Methods Molecular Nucleotides Occupations Organism Orthologous Gene Pathogenesis Pathogenicity Phosphoric Monoester Hydrolases Play Process Production Property Proteins RNA RNA Binding RNA Degradation RNA Processing RNA annealing Research Research Project Grants Ribonucleases Ribosomes Role Scanning Side Site Stress Structure Transcript Untranslated RNA Ursidae Family endonuclease inorganic phosphate insight mRNA Decay mRNA Transcript Degradation novel paralogous gene phosphodiester preference public health relevance ribonuclease E single-molecule FRET

项目摘要

PROJECT SUMMARY DESCRIPTION: The long-term goal of this research project is to understand the basic principles that govern mRNA degradation, a process that has long been known to play a key role in controlling gene expression in all organisms. In Escherichia coli and related bacterial species, the ribonuclease with the greatest influence on mRNA lifetimes is RNase E, an endonuclease with a strong preference for decay intermediates that bear a single phosphate at the 5′ terminus. Recent evidence indicates that RNase E locates its cleavage sites in monophosphorylated RNA by scanning linearly downstream from the 5′ end along RNA segments that are single-stranded. The immediate objectives of this research project are to elucidate the mechanism of RNase E scanning, the enzyme characteristics that make it possible, the features of RNA that facilitate or impede it, and the impact of this process on gene expression. Achieving these objectives will require the use of standard molecular biological, biochemical, and genetic methods as well as single-molecule FRET. The knowledge gained from these studies will provide fundamental insights into a novel aspect of gene regulation that is likely to be important for bacterial pathogenesis.

项目摘要描述：本研究项目的长期目标是了解 mRNA降解的原理，这一过程长期以来一直被认为是在所有生物体中控制基因表达的作用。在大肠杆菌和相关细菌中在物种中，对mRNA寿命影响最大的核糖核酸酶是RNase E，核酸内切酶具有强烈的偏好衰变中间体，携带一个单一的磷酸，第5章终点最近的证据表明，RNase E将其切割位点定位在通过从5 ′端沿着RNA线性扫描下游的单磷酸化RNA 单链的片段。这个研究项目的近期目标是阐明RNase E扫描的机制，使其成为可能的酶特性，可能的，RNA的特征，促进或阻碍它，以及这一过程对基因的影响，表情实现这些目标将需要使用标准的分子生物学，生物化学和遗传学方法以及单分子FRET。获得的知识这些研究将为基因调控的一个新方面提供基本的见解，可能对细菌的致病机理很重要。