Core C - Stem Cell Core

骨干细胞核心(核心C)的主要功能是分离和分配骨髓- 来源的间充质干细胞/祖细胞(BMSCs)给个体研究人员 项目，并为这些单元的使用提供技术支持。一个至关重要的关键组件， 建议项目的成功在于BMSCs的可用性，因为所有项目 需要这些细胞。因此，生产高质量电池的集中核心设施至关重要 为这个动态协调的项目的成功干杯。这一核心将使 个人研究人员通过降低成本，为研究提供质量控制的努力 程序，并加强多个学科之间的协作，以实现更大的 通过综合方法获得科学成果。此外，细胞的生产和分配 将确保所有研究从相同谱系的细胞开始，并且 背景资料。其具体目的是：1.分离、鉴定和分布小鼠骨髓间充质干细胞， 人骨髓间充质干细胞(HMSCs)，以及2.为低氧和 常规的MSC培养和分化(成骨、成脂和成软骨) 化验。通过实现这些目标，该核心将作为一种独特的资源来提供 鼠和人的骨髓间充质干细胞和对个别项目的技术援助，以及 其他对衰老相关研究感兴趣的佐治亚摄政大学研究人员， 代谢性骨骼疾病，或干细胞研究。

The main function of the Bone Stem Cell Core (Core C) is to isolate and distribute bone marrow- derived mesenchymal stem/progenitor cells (BMSCs) to the investigators of the individual projects, and to provide technical support for use of the cells. A key component essential to the success of the proposed Program Project is the availability of BMSCs because all projects require these cells. Thus, a centralized Core facility for the production of quality cells is crucial to the success of this dynamically coordinated Program Project. This Core will benefit the research efforts of individual investigator by reducing costs, providing quality control for study procedures, and enhancing collaborations among multiple disciplines to achieve greater scientific gain through an integrated approach. In addition, production and distribution of cells from a centralized facility will ensure that all studies start with cells of the same lineages and background. The Specific Aims are: 1. Isolate, characterize and distribute mouse BMSCs, human bone marrow MSCs (hMSCs), and 2. Provide ongoing technical support for hypoxic and conventional MSC culture and differentiation (osteogenic, adipogenic, and chondrogenic) assays. By achieving these objectives, this Core will function as a unique resource to provide mouse and human BMSCs and technical assistance to the individual projects, as well as to other Georgia Regents University investigators who are interested in studies related to aging, metabolic bone diseases, or stem cell research.